Disruption of six novel Saccharomyces cerevisiae genes reveals that YGL129c is necessary for growth in non-fermentable carbon sources, YGL128c for growth at low or high temperatures and YGL125w is implicated in the biosynthesis of methionine

Yeast

Volumn 15, Issue 2, 1999, Pages 145-154

  Tizón, Belén ^a   Rodríguez Torres, Ana MARÍA ^a   Cerdán, María Esperanza ^a  

^a UNIVERSITY OF A CORUÑA   (Spain)

Author keywords

DnaJ; Functional analysis; Gene disruption; Methylenetetrahydrofolate reductase; Saccharomyces cerevisae

Indexed keywords

CARBON; FUNGAL DNA; METHIONINE;

ARTICLE; CONTROLLED STUDY; DNA FLANKING REGION; FUNGUS GROWTH; GENE DELETION; GENE DISRUPTION; GENE LOCUS; NONHUMAN; OPEN READING FRAME; PHENOTYPE; PRIORITY JOURNAL; SACCHAROMYCES CEREVISIAE; TEMPERATURE;

CARBON; GENE DELETION; GENES, FUNGAL; METHIONINE; OPEN READING FRAMES; POLYMERASE CHAIN REACTION; SACCHAROMYCES CEREVISIAE; TEMPERATURE; TRANSFORMATION, GENETIC;

FUNGI; SACCHAROMYCES; SACCHAROMYCES CEREVISIAE;

EID: 0345390061     PISSN: 0749503X     EISSN: None     Source Type: Journal    
DOI: 10.1002/(SICI)1097-0061(19990130)15:2<145::AID-YEA346>3.0.CO;2-J     Document Type: Article

References (28)

1. Baudin, A., Ozier-Kalogeropoulos, O.. Denuel, A., Lacrute, F. and Cullin, C. (1993). A simple and efficient method for direct gene deletion in Saccharomyces cerevisiae. Nucl. Acids Res. 21, 3329-3330.
2. Burns, N., Grinwade, B., Ross-Macdonald, P. B. et al. (1994). Large-scale analysis of gene expression, protein localisation and gene disruption in Saccharomyces cerevisiae. Genes Dev. 8, 1087-1105.
3. Caplan, A. J. and Douglas, M. G. (1991). Characterisation of YDJ1: a yeast homologue of the bacterial dnaJ protein. J. Cell. Biol. 114, 609-621.
4. Goffeau, A., Barrell, B. G., Bussey, H. et al. (1996). Life with 6000 genes. Science 274, 546-567.
5. Goyette, P., Christensen, B., Rosenblatt, D. S. and Rozen, R. (1996). Severe and mild mutations in cis for the methylenetetrahydrofolate reductase (MTHFR) gene, and description of five novel mutations in MTHFR. Am. J. Hum. Genet. 59, 1268-1275.
6. Goyette, P., Frosst, P., Rosenblatt, D. S. and Rozen, R. (1995). Seven novel mutations in the methylenetetrahydrofolate reductase gene and genotype-phenotype correlations in severe methylenetetrahydrofolate reductase deficiency. Am. J. Hum. Genet. 56, 1052-1059.
7. Goyette, P., Sumner, J. S., Milos, R., Duncan, A. M., Rosenblatt, D. S., Matthews, R. G. and Rozen, R. (1994). Human methylene-tetrahydrofolate reductase: isolation of cDNA, mapping and mutation identification. Nat. Genet. 7, 195-200.
8. Güldener, U.. Heck, S., Fiedler, T., Beinhauer, J. and Hegemann, J. H. (1996). A new efficient gene disruption cassette for repeated use in budding yeast. Nucl. Acids Res. 24, 2519-2524.
9. Hartl, F. U. (1996). Molecular chaperones in cellular protein folding. Nature 381, 571-579.
10. Huang, M. E., Cadieu, E., Souciet, J. C. and Galibert, F. (1997). Disruption of six novel yeast genes reveals three genes essential for vegetative growth and one required for growth at low temperature. Yeast 13, 1181-1194.
11. Ito, H., Fukuda, Y., Murata, K. and Kimura, A. (1983). Transformation of intact yeast cells treated with alkali cations. J. Bacteriol. 153, 163-168.
12. Kelley, W. L. and Landry, S. J. (1994). Chaperone power in a virus? Trends Biochem. Sci. 19, 277-278.
13. Lawson, J. E. and Douglas, M. G. (1988). Separate genes encode functionally equivalent ADP/ATP carrier proteins in Saccharomyces cerevisiae. Isolation and analysis of AAC2. J. Biol. Chem. 263, 14,812-14,818.
14. Luke, M. M., Sutton, A. and Arndt, K. T. (1991). Characterization of SIS1, a Saccharomyces cerevisiae homologue of bacterial dnaJ proteins. J. Cell. Biol. 114, 623-638.
15. Murakami. H., Blobel, G. and Pain, D. (1990). Signal sequence region of mitochondrial precursor proteins binds to mitochondrial impor receptor. Proc. Natl Acad. Sci. USA 90, 3358-3362.
16. Nelson, D. R., Lawson, J. E., Klingenberg, M. and Douglas, M. G. (1993). Site-directed mutagenesis of the yeast mitochondrial ADP/ATP translocator. Six arginines and one lysine are essential. J. Mol. Biol. 230, 1159-1170.
17. Oliver, S. (1996). A network approach to the systematic analysis of yeast gene function. Trends Genet. 12, 241-242.
18. Rodríguez-Belmonte, E., Rodríguez-Torres, A. M., Tizón, B., Cadahía, J. L., González-Siso, I., Ramil, E., Becerra, M., González-Domínguez, M. and Cerdán, E. (1996). Sequence analysis of a 10 kb DNA fragment from yeast chromosome VII reveals a novel member of the DnaJ family. Yeast 12, 145-148.
19. Rothstein, R. (1991). Targeting, disruption, replacement, and allele rescue: integrative DNA transformation in yeast. Methods Encymol. 194, 281-301.
20. Rowley, N., Prib-Buus, C., Westermann, B., Brown, C., Schwarz, E., Barrel, B. and Neupert, W. (1994). Mdjlp, a novel chaperone of the DnaJ family, is involved in mitochondrial biogenesis and protein folding. Cell 77, 249-259.
21. Sadler, I., Chiang, A., Kurihara, T., Rothblatt, J., Way, J. and Silver, P. (1989). A yeast gene important for protein assembly into the endoplasmic reticulum and the nucleus has homology to DnaJ, an Escherichia coli heat shock protein. J. Cell. Biol. 109, 2665-2675.
22. Saito, H. and Uchida, H. (1978). Organization and expression of the dnaJ and dnaK genes of Escherichia coli K12. Mol. Gen. Genet. 164, 1-8.
23. Sambrook, J., Maniatis, T. and Fritsch, E. F. (1989). Molecular cloning: A Laboratory Manual. Cold Spring Harbor Laboratory Press. New York.
24. Silver, P. A. and Way, J. C. (1993). Eukaryotic DnaJ homologs and the specificity of Hsp70 activity. Cell 74, 5-6.
25. Sunshine, M. (1977). A new host gene (groPC) necessary for lambda DNA replication. Mol. Gen. Genet. 151, 27-34.
26. Tizón, B., Rodríguez-Torres, A. M., Rodríguez-Belmonte, E., Cadahía, J. L. and Cerdán, E. (1996). Identification of a putative methylenetetrahydrofolate reductase by sequence analysis of a 6-8 kb DNA fragment of yeast chromosome VII. Yeast 12, 1047-1051.
27. Wach, A., Brachat, A., Poehlmann, R. and Philippsen, P. (1994). New heterologous modules for classical or PCR-based gene disruptions in Saccharomyces cerevisiae. Yeast 10, 1793-1808.
28. Wu, M., Repetto, B., Glerum, D. M. and Tzagoloff, A. (1995). Cloning and characterization of FAD1, the structural gene for flavin adenine dinucleotide synthetase of Saccharomyces cerevisiae. Mol. Cell. Biol. 15, 264-271.