Determination of endogenous extracellular signal-regulated protein kinase by microchip capillary electrophoresis

Analytical Biochemistry

Volumn 316, Issue 2, 2003, Pages 181-191

  Starkey, Dustin E ^a   Abdelaziez, Yasser ^b   Ahn, Chong H ^b   Tu, Jian ^c   Anderson, LaShonda ^c   Wehmeyer, Kenneth R ^c   Izzo, Nicholas J ^c   Carr, Andrew N ^c   Peters, Kevin G ^c   Bao, James J ^d   Halsall, H Brian ^a   Heineman, William R ^a  

^a UNIVERSITY OF CINCINNATI   (United States)

^b University of Cincinnati   (United States)

^c PROCTER AND GAMBLE PHARMACEUTICALS   (United States)

^d Advanced Medicine Inc   (United States)

Author keywords

Capillary electrophoresis; Enzyme assay; Extracellular signal regulated protein kinase; Microchip

Indexed keywords

AMINO ACIDS; CAPILLARY ELECTROPHORESIS; CELL ENGINEERING; ENDOTHELIAL CELLS; ENZYMES; PEPTIDES; PHOSPHORYLATION;

CONVENTIONAL TECHNIQUES; ENZYME ASSAYS; EXTRACELLULAR SIGNAL-REGULATED PROTEIN KINASE; HUMAN UMBILICAL VEIN ENDOTHELIAL (HUVEC) CELLS; MICROCHIP; MICROCHIP CAPILLARY ELECTROPHORESIS; PHOSPHORYLATED PEPTIDES; VASCULAR ENDOTHELIAL GROWTH FACTOR;

MICROPROCESSOR CHIPS;

5' TRIPHOSPHATE; MITOGEN ACTIVATED PROTEIN KINASE; NONAPEPTIDE; PHOSPHATE; THREONINE; UNCLASSIFIED DRUG; VASCULOTROPIN;

ACCURACY; ANALYTIC METHOD; ARTICLE; CAPILLARY ELECTROPHORESIS; CATALYSIS; CELL STIMULATION; CONCENTRATION (PARAMETERS); CONTROLLED STUDY; CYTOLYSIS; ENDOTHELIUM CELL; ENZYME ACTIVITY; ENZYME ANALYSIS; ENZYME MECHANISM; ENZYME PHOSPHORYLATION; FLUORESCENCE; HUMAN; HUMAN CELL; PRIORITY JOURNAL; PROTEIN ANALYSIS; SAMPLING; STANDARD; TIME; UMBILICAL VEIN;

EID: 0345381940     PISSN: 00032697     EISSN: None     Source Type: Journal    
DOI: 10.1016/S0003-2697(03)00058-7     Document Type: Article

References (60)

1. Morris P.C. MAP kinase signal transduction pathways in plants. New Phytol. 151:2001;67-89.
2. Wilkinson M.G., Millar J.B.A. Control of the eukaryotic cell cycle by MAP kinase signaling pathways. FASEB J. 14:2000;2147-2157.
3. Traverse S., Gomez N., Paterson H., Marshall C., Cohen P. Sustained activation of the mitogen-activated protein (MAP) kinase cascade may be required for differentiation of PC12 cells: comparison of the effects of nerve growth factor and epidermal growth factor. Biochem. J. 288:1992;351-355.
4. Pang L., Decker S.J., Saltiel A.R. Bombesin and epidermal growth factor stimulate the mitogen-activated protein kinase through different pathways in Swiss 3T3 cells. Biochem. J. 289:1993;283-287.
5. 1/2 activation in postcapillary endothelium J. Biol. Chem. 273:1998;4220-4226.
6. Yashima R., Abe M., Tanaka K., Ueno H., Shitara K., Takenoshita S., Sato Y. Heterogeneity of the signal transduction pathways for VEGF-induced MAPKs activation in human vascular endothelial cells. J. Cell. Physiol. 188:2001;201-210.
7. Osman A.A., Pendergrass M., Koval J., Maezono K., Cusi K., Pratipanawatr T., Mandarino L.J. Regulation of MAP kinase pathway activity in vivo in human skeletal muscle. Am. J. Physiol. Endocrinol. Metab. 278:2000;E992-E999.
8. Cobb M.H., Goldsmith E.J. How MAP kinases are regulated. J. Biol. Chem. 270:1995;14843-14846.
9. Alberts B., Bray D., Lewis J., Raff M., Roberts K., Watson J.D. Molecular Biology of the Cell. third ed. 1994;Garland Publishing Inc. New York.
10. 2 is phosphorylated and activated by MAP kinase Cell. 72:1993;269-278.
11. Aronson D., Violan M.A., Dufresne S.D., Zangen D., Fielding R.A., Goodyear L.J. Exercise stimulates the mitogen-activated protein kinase pathway in human skeletal muscle. J. Clin. Invest. 99:1997;1251-1257.
12. Erickson A.K., Payne D.M., Martino P.A., Rossomando A.J., Shabanowitz J., Weber M.J., Hunt D.F., Sturgill T.W. Identification by mass spectrometry of threonine 97 in bovine myelin basic protein as a specific phosphorylation site for mitogen-activated protein kinase. J. Biol. Chem. 265:1990;19728-19735.
13. Seth A., Gonzalez F.A., Gupta S., Raden D.L., Davis R.J. Signal transduction within the nucleus by mitogen-activated protein kinase. J. Biol. Chem. 267:1992;24796-24804.
14. Pulverer B.J., Kyriakis J.M., Avruch J., Nikolakaki E., Woodgett J.R. Phosphorylation of c-jun mediated by MAP kinases. Nature. 353:1991;670-674.
15. TCF by MAP kinase stimulates ternary complex formation at c-fos promoter Nature. 358:1992;414-421.
16. Ostrowski J., Trzeciak L., Kolodziejski J., Bomsztyk K. Increased constitutive activity of mitogen-activated protein kinase and renaturable 85. kDa kinase in human-colorectal cancer Br. J. Cancer. 78:1998;1301-1306.
17. Yau L., Zahradka P. Immunodetection of activated mitogen-activated protein kinase in vascular tissues. Mol. Cell. Biochem. 172:1997;59-66.
18. Kemp B.E., Pearson R.B. Hunter T., Sefton B.M. Methods in Enzymology. vol. 200:1991;121-134 Academic Press, San Diego.
19. Forrer P., Tamaskovic R., Jaussi R. Enzyme-linked immunosorbent assay for measurement of JNK, ERK, and p38 kinase activities. Biol. Chem. 379:1998;1101-1111.
20. Braunwalder A.F., Yarwood D.R., Sills M.A., Lipson K.E. Measurement of the protein tyrosine kinase activity of c-src using time-resolved fluorometry of europium chelates. Anal. Biochem. 238:1996;159-164.
21. Seethala R., Menzel R. A homogeneous, fluorescence polarization assay for src-family tyrosine kinses. Anal. Biochem. 253:1997;210-218.
22. Seethala R., Menzel R. A fluorescence polarization competition immunoassay for tyrosine kinases. Anal. Biochem. 255:1998;257-262.
23. Xue G., Pang H., Yeung E.S. Multiplexed capillary zone electrophoresis and micellar electrokinetic chromatography with internal standardization. Anal. Chem. 71:1999;2642-2649.
24. Gong X., Yeung E.S. An absorption detection approach for multiplexed capillary electrophoresis using a linear photodiode array. Anal. Chem. 71:1999;4989-4996.
25. Kang S.H., Gong X., Yeung E.S. High-throughput comprehensive peptide mapping of proteins by multiplexed capillary electrophoresis. Anal. Chem. 72:2000;3014-3021.
26. Ma L., Gong X., Yeung E.S. Combinatorial screening of enzyme activity by using multiplexed capillary electrophoresis. Anal. Chem. 72:2000;3383-3387.
27. Shi Y., Simpson P.C., Scherer J.R., Wexler D., Skibola C., Smith M.T., Mathies R.A. Radial capillary array electrophoresis microplate and scanner for high-performance nucleic acid analysis. Anal. Chem. 71:1999;5354-5361.
28. Kheterpal I., Mathies R.A. Capillary array electrophoresis DNA sequencing. Anal. Chem. 71:1999;31A-37A.
29. Dai J., Tu J., Anderson L.N., Bao J.J., Liu C., Quay B., Wehmeyer K.R. High-throughput separations using multiplexed capillary electrophoresis with laser-induced fluorescence detection. LCGC. 20:2002;606-617.
30. Dawson J.F., Boland M.P., Holmes C.F.B. A capillary electrophoresis-based assay for protein kinases and protein phosphatases using peptide substrates. Anal. Biochem. 220:1994;340-345.
31. Fujima J.M., Danielson N.D. Determination of creatine kinase activity and phosphocreatine in off-line and on-line modes with capillary electrophoresis. Anal. Chim. Acta. 375:1998;233-241.
32. Wu W.-S., Tsai J.-L. Analysis of src kinase and protein kinase c activity by capillary electrophoresis and laser-induced fluorescence. Anal. Biochem. 269:1999;423-425.
33. Gamble T.N., Ramachandran C., Bateman K.P. Phosphopeptide isomer separation using capillary zone electrophoresis for the study of protein kinases and phosphatases. Anal. Chem. 71:1999;3469-3476.
34. Ban E., Nam H.-S., Yu E., Yoo Y.S. Monitoring of extracellular signal-regulated kinase (ERK) activity with capillary electrophoresis. J. Micro. Sep. 13:2001;332-336.
35. Beijersten I., Westerlund D. Capillary zone electrophoresis and micellar electrokinetic chromatography, with taurodeoxycholate as micellar agent, of protein kinase a peptide substrates. Electrophoresis. 17:1996;161-167.
36. Liu B.-F., Zhang L., Lu Y.-T. Characterization of phosphorylation of a novel protein kinase in rice cells by capillary electrophoresis. J. Chrom. A. 918:2001;401-409.
37. Li H., Wu H.Y., Wang Y., Sims C.E., Allbritton N.L. Improved capillary electrophoresis conditions for the separation of kinase substrates by the laser micropipet system. J. Chrom. B. 757:2001;79-88.
38. Hadd A.G., Raymond D.E., Halliwell J.W., Jacobson S.C., Ramsey J.M. Microchip device for performing enzyme assays. Anal. Chem. 69:1997;3407-3412.
39. Hadd A.G., Jacobson S.C., Ramsey J.M. Microfluidic assays of acetylcholinesterase inhibitors. Anal. Chem. 71:1999;5206-5212.
40. Zugel S.A., Burke B.J., Regnier F.E., Lytle F.E. Electrophoretically mediated microanalysis of leucine aminopeptidase using two-photon excited fluorescence detection on a microchip. Anal. Chem. 72:2000;5731-5735.
41. Starkey D.E., Han A., Bao J.J., Ahn C.H., Wehmeyer K.R., Prenger M.C., Halsall H.B., Heineman W.R. Fluorogenic assay for β-glucuronidase using microchip-based capillary electrophoresis. J. Chrom. B. 762:2001;33-41.
42. Xiong L., Regnier F.E. Channel-specific coatings on microfabricated chips. J. Chrom. A. 924:2001;165-176.
43. Burke B.J., Regnier F.E. Electrophoretically mediated microanalysis of β-galactosidase on microchips. Electrophoresis. 22:2001;3744-3751.
44. Wang J., Chatrathi M.P., Tian B., Polsky R. Microfabricated electrophoresis chips for simultaneous bioassays of glucose, uric acid, ascorbic acid, and acetaminophen. Anal. Chem. 72:2000;2514-2518.
45. Wang J., Ibanez A., Chatrathi M.P., Escarpa A. Electrochemical enzyme immunoassays on microchip platforms. Anal. Chem. 73:2001;5323-5327.
46. Wang J., Chatrathi M.P., Tian B. Microseparation chips for performing multienzymatic dehydrogenase/oxidase assays: simultaneous electrochemical measurement of ethanol and glucose. Anal. Chem. 73:2001;1296-1300.
47. Wang J., Chatrathi M.P., Ibanez A., Escarpa A. Micromachined separation chips with post-column enzymatic reactions of "class" enzymes and end-column electrochemical detection: assays of amino acids. Electroanalysis. 14:2002;400-404.
48. Richter T., Shultz-Lockyear L.L., Oleschuk R.D., Bilitewski U., Harrison D.J. Bi-enzymatic and capillary electrophoretic analysis of non-fluorescent compounds in microfluidic devices: determination of xanthine. Sens. Act. B. 81:2002;369-376.
49. Cohen C.B., Chin-Dixon E., Jeong S., Nikiforov T.T. A microchip-based enzyme assay for protein kinase a. Anal. Biochem. 273:1999;89-97.
50. Xue Q., Wainright A., Gangakhedkar S., Gibbons I. Multiplexed enzyme assays in capillary electrophoretic single-use microfluidic devices. Electrophoresis. 22:2001;4000-4007.
51. Jeong S., Nikiforov T.T. Kinase assay based on thiophosphorylation and biotinylation. BioTechniques. 27:1999;1232-1238.
52. Alarie J.P., Jacobson S.C., Culbertson C.T., Ramsey J.M. Effects of the electric field distribution on microchip valving performance. Electrophoresis. 21:2000;100-106.
53. Adamson N., Riley P.F., Reynolds E.C. The analysis of multiple phosphoseryl-containing casein peptides using capillary zone electrophoresis. J. Chrom. 646:1993;391-396.
54. Grossman P.D., Wilson K.J., Petrie G., Lauer H.H. Effect of buffer pH and peptide composition on the selectivity of peptide separations by capillary zone electrophoresis. Anal. Biochem. 173:1988;265-270.
55. Wehr T., Rodriguez-Diaz R., Zhu M. Capillary Electrophoresis of Proteins. Chromatographic Science Series. vol. 80:1999;Marcel Dekker Inc. New York.
56. mpk, the meiosis-activated myelin basic protein kinase J. Biol. Chem. 23:1991;15180-15184.
57. Shultz-Lockyear L.L., Colyer C.L., Fan Z.H., Roy K.I., Harrison D.J. Effects of injector geometry and sample matrix on injection and sample loading in integrated capillary electrophoresis devices. Electrophoresis. 20:1999;529-538.
58. Copeland R.A. Enzymes: A Practical Introduction to Structure, Mechanism, and Data Analysis. 1996;Wiley-VCH Inc. New York.
59. Quinn T.P., Peters K.G., DeVries C., Ferrara N., Williams L.T. Fetal liver kinase 1 is a receptor for vascular endothelial growth factor and is selectively expressed in vascular endothelium. Proc. Natl. Acad. Sci. USA. 90:1993;7533-7537.
60. Gupta K., Kshirsagar S., Li W., Gui L., Ramakrishnan S., Gupta P., Law P.Y., Hebbel R.P. VEGF prevents apoptosis of human microvascular endothelial cells via opposing effects on MAPK/ERK and SAPK/JNK signaling. Exp. Cell Res. 247:1999;495-504.