Quantitative assay system for specific enzyme activity using antibody: The case of protease, subtilisin BPN

Journal of Biotechnology

Volumn 66, Issue 2-3, 1998, Pages 157-163

  Miyota, Yoshiaki ^a   Komada, Shingo ^a   Momose, Haruo ^a   Taguchi, Seiichi ^a  

^a TOKYO UNIVERSITY OF SCIENCE   (Japan)

Author keywords

Antibody bound enzyme assay; Evolutionary engineering; Random screening; Specific enzyme activity; Subtilisin BPN

Indexed keywords

ANTIBODIES; BACTERIA; BIOASSAY; CATALYST ACTIVITY; CELL CULTURE; GENETIC ENGINEERING; HYDROLYSIS;

ANTIBODY-BOUND ENZYME ASSAY; BACTERIAL SERINE PROTEASE; EVOLUTIONARY ENGINEERING; POLYCLONAL ANTIBODIES;

ENZYMES;

PROTEINASE; SUBTILISIN;

ARTICLE; BIOENGINEERING; BIOTECHNOLOGY; CONTROLLED STUDY; ENZYME ACTIVITY; ENZYME ASSAY; ENZYME SPECIFICITY; GENETIC ENGINEERING; NONHUMAN; PRIORITY JOURNAL; PROTEIN DEGRADATION; QUANTITATIVE ASSAY; SCREENING;

ANIMALS; ANTIBODIES; BACILLUS SUBTILIS; BIOCHEMISTRY; BLOTTING, WESTERN; ELECTROPHORESIS, POLYACRYLAMIDE GEL; MUTATION; RABBITS; RECOMBINANT PROTEINS; SUBTILISINS;

EID: 0345367460     PISSN: 01681656     EISSN: None     Source Type: Journal    
DOI: 10.1016/S0168-1656(98)00148-5     Document Type: Article

References (12)

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