Quantification of uridine 5′-diphosphate (UDP)-glucose by high-performance liquid chromatography and its application to a nonradioactive assay for nucleoside diphosphate kinase using UDP-glucose pyrophosphorylase as a coupling enzyme

Analytical Biochemistry

Volumn 323, Issue 2, 2003, Pages 188-196

  Dorion, Sonia ^a   Rivoal, Jean ^a  

^a UNIVERSITÉ DE MONTRÉAL   (Canada)

Author keywords

Coupled enzyme assay; HPLC; Ion exchange chromatography; Nucleoside diphosphate kinase; Sucrose synthase; Uridine 5 diphosphoglucose; Uridine 5 diphosphoglucose pyrophosphorylase; Uridine 5 triphosphate

Indexed keywords

BIOMOLECULES; CHROMATOGRAPHIC ANALYSIS; COLUMN CHROMATOGRAPHY; GLUCOSE; HIGH PERFORMANCE LIQUID CHROMATOGRAPHY; ION CHROMATOGRAPHY; ION EXCHANGE;

ENZYME ASSAYS; HPLC; ION EXCHANGE CHROMATOGRAPHY; NUCLEOSIDE-DIPHOSPHATE KINASE; SUCROSE SYNTHASE; TRIPHOSPHATE;

ENZYMES;

GLUCOSE 1 PHOSPHATE; ISOENZYME; NUCLEOSIDE DIPHOSPHATE KINASE; PYRUVATE KINASE; URIDINE DIPHOSPHATE GLUCOSE; URIDINE TRIPHOSPHATE; URIDINE TRIPHOSPHATE GLUCOSE 1 PHOSPHATE URIDYLYLTRANSFERASE;

ANIMAL TISSUE; ANION EXCHANGE CHROMATOGRAPHY; ARTICLE; CATALYSIS; CATALYST; CONTROLLED STUDY; ENZYME ACTIVITY; ENZYME ASSAY; ENZYME KINETICS; ENZYME MECHANISM; HIGH PERFORMANCE LIQUID CHROMATOGRAPHY; INCUBATION TIME; NONHUMAN; NUCLEOTIDE SEQUENCE; PRIORITY JOURNAL; RAT; RELIABILITY; REPRODUCIBILITY; SEPARATION TECHNIQUE; SPECTROPHOTOMETRY;

ANIMALIA;

EID: 0345356393     PISSN: 00032697     EISSN: None     Source Type: Journal    
DOI: 10.1016/j.ab.2003.08.034     Document Type: Article

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