Three-dimensional Organization of pKi-67: A Comparative Fluorescence and Electron Tomography Study Using Fluoronanogold

Journal of Histochemistry and Cytochemistry

Volumn 51, Issue 11, 2003, Pages 1411-1423

  Cheutin, Thierry ^a,e   O'Donohue, Marie Françoise ^a   Beorchia, Adrien ^b   Klein, Christophe ^c   Kaplan, Hervé ^d   Ploton, Dominique ^a  

^a CNRS   (France)

^b UNIVERSITÉ DE REIMS CHAMPAGNE ARDENNE   (France)

^c INSERM   (France)

^d IFR 53   (France)

^e NATIONAL CANCER INSTITUTE   (United States)

Author keywords

Confocal microscopy; Electron tomography; Heterochromatin; Ki 67 antigen; Nucleolus

Indexed keywords

DNA; FLUORESCENT DYE; MONOCLONAL ANTIBODY KI 67;

ARTICLE; CONFOCAL MICROSCOPY; CONTROLLED STUDY; FLUORESCENCE; HETEROCHROMATIN; HUMAN; HUMAN CELL; IMMUNOCYTOCHEMISTRY; IMMUNOLOCALIZATION; INTERPHASE; PRIORITY JOURNAL; SCANNING TRANSMISSION ELECTRON MICROSCOPY; THREE DIMENSIONAL IMAGING; ULTRASTRUCTURE;

EID: 0345256296     PISSN: 00221554     EISSN: None     Source Type: Journal    
DOI: 10.1177/002215540305101102     Document Type: Article

References (56)

1. Bahr GF, Boccia JA, Engler WF (1979) Reconstruction of a chromosome model from its projections. Ultramicroscopy 4:45-53
2. Baschong W, Stierhof YD (1998) Preparation use and enlargement of ultrasmall gold particles in immunoelectron microscopy. Microsc Res Tech 42:66-79
3. Baumeister W, Grimm R, Walz J (1999) Electron tomography of molecules and cells. Trends Cell Biol 9:81-85
4. Bednar J, Horowitz RA, Grigoryev SA, Carruthers LM, Hansen JC, Koster AJ, Woodcock CL (1998) Nucleosomes linker DNA and linker histone form a unique structural motif that directs the higher-order folding and compaction of chromatin. Proc Natl Acad Sci USA 95:14173-4178
5. Belmont AS, Dietzel S, Nye AC, Strukov YG, Tumbar T (1999) Large-scale chromatin structure and function. Curr Op Cell Biol 11:307-311
6. Beorchia A, Héliot L, Ménager M, Kaplan H, Ploton D (1992) Applications of medium-voltage STEM for the 3-D study of organelles within thick sections. J Microsc 170:247-258
7. Bridger J, Kill I, Lichter P (1998) Association of pKi-67 with satellite DNA of the human genome in early G1 cells. Chromosome Res 6:13-24
8. Cheutin T, O'Donohue MF, Beorchia A, Vandelaer M, Kaplan H, Deféver B, Ploton D, et al. (2002) Three-dimensional organization of active rRNA genes within the nucleolus. J Cell Sci 115:3297-3307
9. Crowther RA, de Rosier DJ, Klug A (1970) The reconstruction of a three-dimensional structure from projections and its application to electron microscopy. Proc R Soc Lond [A] 317:319-340
10. Derenzini M, Thiry M, Goessens G (1990) Ultrastructural cytochemistry of the mammalian cell nucleus. J Histochem Cytochem 38:1237-1256
11. Derenzini M, Viron A, Puvion-Dutilleul F (1982) The Feulgen-like osmium-ammine reaction as a tool to investigate chromatin structure in thin sections. J Ultrastruct Res 80:133-147
12. Duchrow M, Gerdes J, Schlüter C (1994) The proliferation-associated Ki-67 protein: definition in molecular terms. Cell Prolif 27:235-242
13. Endl E, Gerdes J (2000) The Ki-67 protein: fascinating forms and unknown functions. Exp Cell Res 257:231-237
14. Frank J, Wagenknecht T, McEwen BF, Marko M, Hsieh CE, Mannella C (2002) Three-dimensional imaging of biological complexity. J Struct Biol 138:85-91
15. Gerdes J, Lemke H, Baisch H, Wacker HH, Schwab U, Stein H (1984) Cell cycle analysis of a cell proliferation-associated human nuclear antigen defined by the monoclonal antibody Ki-67. J Immunol 133:1710-1715
16. Gerdes J, Li L, Schlueter C, Duchrow M, Wohlenberg C, Gerlach C, Stahmer I, et al. (1991) Immunobiochemical and molecular biologic characterization of the cell proliferation associated nuclear antigen that is defined by monoclonal antibody Ki-67. Am J Pathol 138:867-873
17. Gerdes J, Schwab U, Lemke H, Stein H (1983) Production of a mouse monoclonal antibody reactive with a nuclear antigen associated with cell proliferation. Int J Cancer 31:13-20
18. Gordon R, Bender R, Herman GT (1970) Algebraic reconstruction techniques (ART) for the three-dimensionnal electron microscopy and x-ray photography. J Theor Biol 29:471-481
19. Hainfeld JF, Furuya FR (1992) A 14-nm gold cluster covalently attached to antibodies improves immunolabeling. J Histochem Cytochem 40:177-184
20. Hainfeld JF, Powell RD (2000) New frontiers in gold labeling. J Histochem Cytochem 48:471-480
21. Héliot L, Kaplan H, Lucas L, Klein C, Beorchia A, Doco-Fenzy M, Ménager M, et al. (1997) Electron tomography of metaphase nucleolar organizer regions: evidence for a twisted-loop organization. Mol Biol Cell 8:2199-2216
22. Henckel J, Fersht AR, Jackson SP (1999) The FHA domain is a modular phosphopeptide recognition motif. Mol Cell 4:387-394
23. Hofmann K, Bucher P (1995) The FHA domain: a putative nuclear signaling domain found in protein kinases and transcription factors. Trends Biochem Sci 20:347-349
24. Humbel BM, De Jong MDM, Müller WH, Verkleij AJ (1998) Preembedding immunolabeling for electron microscopy: an evaluation of permeabilization methods and markers. Microsc Res Tech 42:43-58
25. Isola J, Helin H, Kallioniemi OP (1990) Immunoelectron-microscopic localization of a proliferation-associated antigen Ki-67 in MCF-7 cells. J Pathol 162:295-301
26. Kametaka A, Takagi M, Hayakawa T, Haraguchi T, Hiraoka Y, Yoneda Y (2002) Interaction of the chromatin compaction-inducing domain (LR domain) of Ki-67 with HP1 proteins. Genes Cells 7:1231-1242
27. Kill I (1996) Localization of the Ki-67 antigen within the nucleolus. Evidence for a fibrillarin-deficient region of the dense fibrillar component. J Cell Sci 109:1253-1263
28. Klein C, Cheutin T, O'Donohue M, Rothblum L, Kaplan H, Beorchia A, Lucas L, et al. (1998) The three-dimensional study of chromosomes and of upstream binding factor-immunolabelled nucleolar organizer regions demonstrates their nonrandom spatial arrangement during mitosis. Mol Biol Cell 9:3147-3159
29. Kornberg RD, Lorch Y (2002) Chromatin and transcription: where do we go from here? Curr Opin Genet Dev 12:249-251
30. Kourmouli N, Theorodopoulos PA, Dialynas G, Bakou A, Politou AS, Cowell IG, Singh PB, et al. (2000) Dynamic associations of heterochromatin protein 1 with the nuclear envelope. EMBO J 19:6558-6568
31. Kreitz S, Fackelmayer FO, Gerdes J, Knippers R (2000) The proliferation-specific human Ki-67 protein is a constituent of compact chromatin. Exp Cell Res 261:284-292
32. Landberg G, Roos G (1993) Proliferating cell nuclear antigen and Ki-67 antigen expression in human haematopoietic cells during growth stimulation and differentiation. Cell Prolif 26:427-437
33. Li J, Lee GI, van Doren SR, Walker JC (2000) The FHA domain mediates phosphoprotein interactions. J Cell Sci 113:4143-4149
34. Lopez F, Belloc F, Lacombe F, Dumain P, Reiffers J, Bernard P, Boisseau MR (1991) Modalities of synthesis of Ki67 antigen during the stimulation of lymphocytes. Cytometry 12:42-49
35. Lopez F, Belloc F, Lacombe F, Dumain P, Reiffers J, Bernard P, Boisseau MR (1994) The labeling of proliferating cells by Ki-67 and Mib-1 antibodies depends on the binding of a nuclear protein to the DNA. Exp Cell Res 210:145-153
36. Lucas L, Gilbert N, Ploton D, Bonnet N (1996) Visualization of volume data in confocal microscopy: comparison and improvements of volume rendering methods. J Microsc 181:238-252
37. MacCallum DE, Hall PA (2000) The biochemical characterization of the DNA binding activity of pKi-67. J Pathol 191:286-298
38. O'Donohue MF, Cheutin T, Klein C, Kaplan H, Ploton D (1998) A mounting technique allows observation of immunolabelled cells on plastic coverslips. BioTechniques 24:910-914
39. Perkins GA, Renken CW, Song JY, Frey TG, Young SJ, Lamont S, Martone ME, et al. (1997) Electron tomography of large multicomponent biological structures. J Struct Biol 120:219-227
40. Robinson JM, Takizawa T, Vandré DD (2000) Applications of gold cluster compounds in immunocytochemistry and correlative microscopy: comparison with colloidal gold. J Microsc 199:163-179
41. Robinson JM, Vandré DD (1997) Efficient immunocytochemical labeling of leukocyte microtubules with FluoroNanogold: an important tool for correlative microscopy. J Histochem Cytochem 45:631-642
42. Sadoni N, Sullivan KF, Weinzierl P, Stelzer EHK, Zink D (2001) Large-scale chromafin fibers of living cells display a discontinuous functional organization. Chromosoma 110:39-51
43. Sasaki K, Murakami T, Kawasaki M, Takahashi M (1987) The cell cycle associated change of the Ki-67 reactive nuclear antigen expression. J Cell Physiol 133:579-584
44. Schlüter C, Duchrow M, Wohlenberg C, Becker MHG, Key G, Flad HD, Gerdes J (1993) The cell proliferation-associated antigen of antibody Ki-67: a very large ubiquitous nuclear protein with numerous repeated elements representing a new kind of cell cycle-maintaining proteins. J Cell Biol 123:513-522
45. Scholzen T, Endl E, Wohlenberg C, van der Sar S, Cowell IG, Gerdes J, Singh PB (2002) The Ki-67 protein interacts with members of the heterochromatin protein 1 (HP1) family: a potential role in the regulation of the higher-order chromatin structure. J Pathol 196:135-144
46. Scholzen T, Gerdes J (2000) The Ki-67 protein: from the known and the unknown. J Cell Physiol 182:311-322
47. Starborg M, Gell K, Brundell E, Höög C (1996) The murine Ki-67 cell proliferation antigen accumulates in the nucleolar and heterochromatic regions of interphase cells and at the periphery of the mitotic chromosomes in a process essential for cell cycle progression. J Cell Sci 109:143-153
48. Sueishi M, Takagi M, Yoneda Y (2000) The forkhead-associated domain of Ki-67 antigen interacts with the novel kinesin-like protein Hklp2. J Biol Chem 275:28888-28892
49. Takagi M, Matsuoka Y, Kurihara T, Yoneda Y (1999) Cmadrin: a novel Ki-67 antigen-related perichromosomal protein possibly implicated in higher order chromatin structure. J Cell Sci 112:2463-2472
50. Takagi M, Sueishi M, Saiwaki T, Kametaka A, Yoneda Y (2001) A novel nucleolar protein NIFK interacts with the forkhead associated domain of Ki-67 antigen in mitosis. J Biol Chem 276:25386-25391
51. Thiry M, Goessens G (1996) The nucleolus during the cell cycle. In Landes RG, ed. Molecular Biology Intelligence Unit. Heidelberg, Springer-Verlag, 1-144
52. Traut W, Endl E, Scholzen T, Gerdes J, Winking H (2002) The temporal and spatial distribution of the proliferative associated Ki-67 protein during female and male meiosis. Chromosoma 111:156-164
53. Tumbar T, Sudlow G, Belmont AS (1999) Large scale chromatin unfolding and remodelling induced by VP16 acidic activation domain. J Cell Biol 145:1341-1354
54. van Dierendonck JH, Keijzer R, van de Velde CJH, Cornelisse CJ (1989) Nuclear distribution of the Ki-67 antigen during the cell cycle: comparison with growth fraction in human breast cancer cells. Cancer Res 49:2999-3006
55. Verheijen R, Kuijpers HJH, Schlingemann RO, Boehmer ALM, van Driel R, Brakenhoff GJ, Ramaekers FCS (1989) Ki-67 detects a nuclear matrix-associated proliferation-related antigen I-intracellular localization during interphase. J Cell Sci 92:123-130
56. Woodcock CL, Dimitrov S (2001) Higher-order structure of chromatin and chromosomes. Curr Opin Genet Dev 11:130-135