Organotypic spinal cord culture in serum-free fibrin gel: A new approach to study three-dimensional neurite outgrowth and of neurotoxicity testing: Effects of modulating the actin and tubulin dynamics and protein kinase activities

Journal of Neuroscience Methods

Volumn 78, Issue 1-2, 1997, Pages 93-103

  Rösner, Harald ^a   Vacun, Gabriele ^a  

^a UNIVERSITY OF HOHENHEIM   (Germany)

Author keywords

Computer based densitometry; Drug effects; Fibrin matrix; Neurite outgrowth; Spinal cord

Indexed keywords

1 (5 CHLORO 1 NAPHTHALENESULFONYL)HEXAHYDRO 1H 1,4 DIAZEPINE; 2 [1 (3 AMIDINOTHIOPROPYL) 1H INDOL 3 YL] 3 (1 METHYL 1H INDOL 3 YL)MALEIMIDE; ACTIN; CYTOCHALASIN; DIOCTANOIN; FIBRIN; NOCODAZOLE; PACLITAXEL; PROTEIN KINASE; PROTEIN KINASE C; TETRAMETHYLLEAD; TUBULIN;

ANIMAL CELL; ANIMAL TISSUE; ARTICLE; CELL CULTURE; CHICK EMBRYO; CONTROLLED STUDY; EMBRYO; MOLECULAR DYNAMICS; NERVE CELL; NERVE FIBER GROWTH; NEUROTOXICITY; NONHUMAN; PRIORITY JOURNAL; SPINAL CORD SURGERY; THREE DIMENSIONAL IMAGING; TOXICITY TESTING;

ACTINS; ANIMALS; APROTININ; AZEPINES; CHICK EMBRYO; CYTOCHALASIN D; DENSITOMETRY; DIGLYCERIDES; ENZYME INHIBITORS; FIBRIN; GELS; INDOLES; NEURITES; NEUROTOXINS; NOCODAZOLE; ORGAN CULTURE TECHNIQUES; PACLITAXEL; PROTEIN KINASES; SPINAL CORD; TETRAETHYL LEAD; TUBULIN;

EID: 0345195990     PISSN: 01650270     EISSN: None     Source Type: Journal    
DOI: 10.1016/S0165-0270(97)00132-5     Document Type: Article

References (40)

1. Bär P.R., Roukema G.H., Veraart C.M., Hol E.M., Gispen W.H. Nimodipine protects cultured spinal cord neurons from depolarization-induced inhibition of neurite outgrowth. Cell Calcium. 14:1993;293-299.
2. Bray D., Thomas C., Shaw G. Growth cone formation in cultures of sensory neurons. Proc Natl Acad Sci USA. 75:1978;5226-5229.
3. Doherty P., Walsh F.S. Signal transduction events underlying neurite outgrowth stimulated by cell adhesion molecules. Curr Op Neurobiol. 4:1994;49-55.
4. Dribin L.B., Barrett J.N. Conditioned medium enhances neurite outgrowth from rat spinal cord explants. Dev Biol. 74:1980;184-185.
5. Ebens A., Brose K., Leonardo E.D., Hanson M.G. jr., Bladt F., Birchmeier C., Barres B.A., Tessier-Lavigne M. Hepatocyte growth factor/scatter factor is an axonal chemoattractant and a neurotrophic factor for spinal cord neurons. Neuron. 17:1996;1157-1172.
6. Frosch M., Gorgen I., Boulnois G.J., Timmis K.N., Bitter-Suermann D. NBZ mouse system for production of monoclonal antibodies to weak bacterial antigens: isolation of an IgG antibody to the polysaccharide capsules of Escherichia coli K1 and group B meningococci. Proc Natl Acad Sci USA. 82:1985;1194-1198.
7. Go M.K., Sekiguchi N., Hideoki N., Kikkawa U., Nishizuka Y. Further studies on the specifity of diacylglycerol for protein kinase C activation. Biochem Biophys Res Commun. 144:1987;598-605.
8. Greis C., Rösner H. C-pathway polysialogangliosides in the nervous tissue of vertebrates, reacting with the monoclonal antibody Q211. Brain Res. 517:1990;105-110.
9. 4-9 analog against the toxic effects of cisplatin and taxol on sensory neurons and glial cells in vitro. J Neurosci Res. 39:1994;178-185.
10. Hsu L., Jeng A.Y., Chen K.Y. Induction of neurite outgrowth from chick embryonic ganglia explants by activation of protein kinase C. Neurosci Lett. 99:1989;257-262.
11. Jessell T.M. Adhesion molecules and the hierarchy of neuronal development. Neuron. 1:1988;3-13.
12. Jian X., Hidaka H., Schmidt J.T. Kinase requirement for retinal growth cone motility. J Neurobiol. 25:1994;1310-1328.
13. Joosten E.A.J., Gispen W.H., Bär P.R. Tropism and corticospinal target selection in the rat. Neuroscience. 59:1994;33-41.
14. Letourneau P.C. Chemotactic response of nerve fiber elongation to nerve growth factor. Dev Biol. 66:1978;183-196.
15. Letourneau P.C., Ressler A.H. Inhibition of neurite initiation and growth by taxol. J Cell Biol. 98:1984;1355-1362.
16. Letourneau P.C., Shattuk T.A., Ressler A.H. 'Pull' and 'push' in neurite elongation: observations on the effect of different concentrations of cytochalasin B and taxol. Cell Motil Cytoskeleton. 8:1987;193-209.
17. Mandys V., van der Neut R., Bär R.P., Gispen W.H. Cultivation of rat fetal spinal cord slices in a semi-solid medium: a new approach to studying axonal outgrowth and regeneration. J Neurosci Methods. 38:1991;63-69.
18. Mandys V., Turecek R., Gispen W.H., Bär P.R. Organotypic cultures of chick dorsal root ganglia in a semi-solid medium: a model for neurotoxicity testing. Toxicol Vitro. 8:1994;81-90.
19. Manfredi J.J., Parness J., Horwitz S.B. Taxol binds to cellular microtubules. J Cell Biol. 94:1982;688-696.
20. Matus A., Bernhardt R., Bodmer R., Alaimo D. Microtubule-associated protein 2 and tubulin are differently distributed in the dendrites of developing neurons. Neuroscience. 17:1986;371-389.
21. Mitchison T., Kirschner M. Cytoskeletal dynamics and nerve growth. Neuron. 1:1988;761-772.
22. Monard D. Cell derived proteases and protease inhibitors as regulators of neurite growth. TINS. 11:1988;541-544.
23. Oakley R.A., Tosney K.W. Contact-mediated mechanisms of motor axon segmentation. J Neurosci. 13:1993;3773-3792.
24. Patterson P.H. On the role of proteases, their inhibitors and the extracellular matrix in promoting neurite outgrowth. J Physiol Paris. 80:1985;207-211.
25. Röderer G., Doenges K.H. Influence of lead on the in vitro assembly of microtubules from mammalian brain. Eur J Cell Biol. 2:1983;35-42.
26. Rösner H., Fischer H. In growth cones of rat cerebral neurons and human neuroblastoma cells, activation of protein kinase C causes a shift from filopodial to lamellipodial actin dynamics. Neurosci Lett. 219:1996;175-178.
27. Rösner H., Vacun G., Rebhan M. Muscarinic receptor-mediated induction of actin-driven lamellar protrusions in neuroblastoma cell somata and growth cones. Involvement of protein kinase C. Eur J Cell Biol. 66:1995;324-334.
28. Saitoh M., Ishikawa T., Matsushima S., Naka M., Hidaka H. Selective inhibition of catalytic activity of smooth muscle myosin-light chain kinase. J Biol Chem. 262:1987;7796-7801.
29. Schiff P.B., Horwitz S.B. Taxol stabilizes microtubules in mouse fibroblast cells. Proc Natl Acad Sci USA. 77:1980;1561-1565.
30. Schliwa M. Action of cytochalasin D on cytoskeletal networks. J Cell Biol. 92:1982;79-81.
31. Serafini T., Kennedy T.E., Galko M.J., Mirzayan C., Jessell T.M., Tessier-Lavigne M. The netrins define a family of axon outgrowth-promoting proteins homologous to C. elegans UNC-6. Cell. 78:1994;409-424.
32. Shirasaki R., Mirzayan C., Tessier-Lavigne M., Murakami F. Guidance of circumferentially axons by netrin-dependent and -independent floor plate chemotropism in the vertebrate brain. Neuron. 17:1996;1079-1088.
33. Sonnentag U., Rösner H., Rahmann H. Influence of exogenous gangliosides on the three-dimensional sprouting of goldfish retinal explants in vitro. Neurosci Res. 17:1992;1105-1112.
34. Tanaka E., Ho T., Kirschner M.W. The role of microtubule dynamics in growth cone motility and axonal growth. J Cell Biol. 128:1995;139-155.
35. Tanaka H., Sakai M., Obata K. Effects of serum, tissue extract, conditioned medium and culture substrata on neurite appearance from spinal cord explants of chick embryo. Dev Brain Res. 4:1982;303-312.
36. Tessier-Lavigne M. Axon guidance by molecular gradients. Curr Opin Neurobiol. 2:1992;60-65.
37. Theodore L., Derossi D., Chassaing G., Llirbat B., Kubes M., Jordan P., Chneiweiss H., Godement P., Prochiantz A. Intraneural delivery of protein kinase C pseudosubstrate leads to growth cone collapse. J Neurosci. 15:1995;7158-7167.
38. Trachsel S., Keller H.U. Selective responses (actin-polymerization, shape changes, locomotion, pinocytosis) to the PKC inhibitor RO-31-8220 suggests that PKC discriminantely regulates function of human blood Iymphocytes. J Leukocyte Biol. 57:1995;587-591.
39. Tuttle R., Matthew W.D. An in vitro bioassay for neurite growth using cryostate sections of nervous tissue as substratum. J Neurosci Methods. 39:1991;193-202.
40. Walter J., Kern-Veits B., Huf J., Stolze B., Bonhoeffer F. Recognition of position-specific properties of tectal membranes by retinal axon in vitro. Development. 101:1987;685-696.