Effect of enamel matrix derivative on human periodontal fibroblasts: Proliferation, morphology and root surface colonization. An in vitro study

Journal of Periodontal Research

Volumn 38, Issue 6, 2003, Pages 568-574

  Cattaneo, V ^a,c,d   Rota, C ^a   Silvestri, M ^a,d   Piacentini, C ^a   Forlino, A ^a   Gallanti, A ^a   Rasperini, G ^b   Cetta, G ^a  

^a UNIVERSITY OF PAVIA   (Italy)

^b UNIVERSITY OF MILAN   (Italy)

^c Studio Ass   (Italy)

^d NONE   (Italy)

Author keywords

Enamel matrix derivative proteins; Fibroblast differentiation; Fibroblast proliferation; Periodontal ligament fibroblasts

Indexed keywords

ALKALINE PHOSPHATASE; EMDOGAIN; ENAMEL PROTEIN; ENAMEL PROTEIN MATRIX DERIVATIVE; UNCLASSIFIED DRUG;

ADULT; ARTICLE; CELL COUNT; CELL CULTURE; CELL DIFFERENTIATION; CELL GROWTH; CELL PROLIFERATION; CELL STRUCTURE; ENZYME ACTIVITY; FEMALE; FIBROBLAST; HUMAN; HUMAN CELL; PERIODONTAL LIGAMENT; SCANNING ELECTRON MICROSCOPY; STATISTICAL SIGNIFICANCE; TOOTH EXTRACTION; TOOTH ROOT CANAL;

EID: 0345166875     PISSN: 00223484     EISSN: None     Source Type: Journal    
DOI: 10.1034/j.1600-0765.2003.00690.x     Document Type: Article

References (50)

1. Nyman S, Gottlow J, Karring T, Lindhe J. The regenerative potential of the periodontal ligament. An experimental study in the monkey. J Clin Periodontol 1982;9: 257-265.
2. Nalbadian J, Frank RM. Electron microscopic study of the cementum and periodontal connective tissue attachment in the cat. J Periodont Res 1980;15:71-89.
3. Listgarten MA. Electron microscopic study of the junction between surgically denuded root surfaces and regenerated periodontal tissue. J Periodont Res 1972;7: 68-90.
4. Araujo M, Berglundh T, Lindhe J. The periodontal tissues in healed degree III furcation defects. An experimental study in dogs. J Clin Periodontol 1996;23:532-541.
5. Listgarten MA, Rosenberg MM. Histological study of repair following new attachment procedures in human periodontal lesions. J Periodontol 1979;50:333-344.
6. Nyman S, Lindhe J, Karring T, Rylander H. New attachment following surgical treatment of human periodontal disease. J Clin Periodontol 1982;9:290-296.
7. Gottlow J, Nyman S, Karring T, Lindhe J. New attachment formation as the result of controlled tissue regeneration. J Clin Periodontol 1984;11:494-503.
8. Caffesse RG, Nasjleti CE, Anderson GB, Lopatin DE, Smith BA, Morrison EC. Periodontal healing following guided tissue regeneration with citric acid and fibronectin application. J Periodontol 1991; 62:21-29.
9. Schroeder H. Biological problems of regenerative cementogenesis: synthesis and attachment of collagenous matrices on growing and established root surfaces. Int Rev Cytol 1992;142:1-59.
10. Luder HU, Zappa U. Nature and attachment of cementum formed under guided conditions in human teeth. An electron microscopic study. J Periodontol 1998; 69:889-898.
11. Yamamoto T, Domon T, Takahashi S, Islam N, Suzuki R, Wakita M. The structure and function of the cementodentinal junction in human teeth. J Periodont Res 1999;34:261-268.
12. Slavkin HC. Towards a cellular and molecular understanding of periodontics. Cementogenesis revisited. J Periodontol 1976;47:249-255.
13. Lindskog S, Hammarström L. Formation of intermediate cementum III,: 3H-proline and 3H-tryptophan uptake into the epithelial root sheath of Hertwig in vitro. J Craniofac Genet Dev Biol 1982;2: 171-177.
14. Hammarström L, Heijl L, Gestrelius S. Periodontal regeneration in a buccal dehiscence model in monkeys after application of enamel matrix proteins. J Clin Periodontol 1997;24:669-677.
15. Heijl L. Periodontal regeneration with enamel matrix derivative in one human experimental defect. A case report. J Clin Periodontol 1997;24:693-696.
16. Heijl L, Heden G, Swärdström G, Östgren A. Enamel matrix derivative (EMDOGAIN) in the treatment of intrabony periodontal defects. J Clin Periodontol 1997; 24:705-714.
17. Hammarström L. Enamel matrix cementum development and regeneration. J Clin Periodontol 1997;24:658-668.
18. Fisher LW, Termine JD. Noncollagenous proteins influencing the local mechanisms of calcification. J Clin Orthod 1985;200: 362-385.
19. Aoba T, Fukae M, Tanabe T, Shimizu M, Moreno EC. Selective adsorption of porcine amelogenins onto hydroxyapatite and their inhibitory activity on hydroxyapatite growth in supersaturated solutions. Calcif Tissue Int 1987;41:281-289.
20. Gestrelius S, Andersson C, Persson E, Brodin A, Rydhag L, Hammarström L. Formulation of enamel matrix derivative for surface coating. Kinetics and cell colonization. J Clin Periodontol 1997;24: 678-684.
21. Brookes SJ, Robinson C, Kirkham J, Bonass WA. Biochemistry and molecular biology of amelogenin proteins of developing dental enamel. Arch Oral Biol 1995; 40:1-14.
22. Silvestri M, Sartori S, Rasperini G, Ricci G, Rota C, Cattaneo V. Comparison of infrabony defects treated with enamel matrix derivative versus guided tissue regeneration with a non-resorbable membrane. A multicenter controlled clinical trial. J Clin Periodontol 2003;5:386-393.
23. Sculean A, Chiantella GC, Windisch P, Gera I, Reich E. Clinical evaluation of an enamel matrix protein derivative (Emdogain) combined with a bovine-derived xenograft (Bio-Oss) for the treatment of intrabony periodontal defects in humans. Int J Perindontics Restorative Dent 2002; 22:259-267.
24. Velasquez-Plata D, Scheyer ET, Mellonig JT. Clinical comparison of an enamel matrix derivative used alone or in combination with a bovine-derived xenograft for the treatment of periodontal osseous defects in humans. J Periodontol 2002;73: 433-440.
25. Lekovic V, Camargo PM, Weinlaender M, Vasilic N, Djordjevic M, Kenney EB. The use of bovine porous bone mineral in combination with enamel matrix proteins or with an autologous fibrinogen/fibronectin system in the treatment of intrabony periodontal defects in humans. J Periodontol 2001;72:1157-1163.
26. Silvestri M, Ricci G, Rasperini G, Sartori S, Cattaneo V. Comparison of treatments of infrabony defects with enamel matrix derivative, guided tissue regeneration with a nonresorbable membrane and Widman modified flap. A pilot study. J Clin Periodontol 2000;8:603-610.
27. Rasperini G, Ricci G, Silvestri M. Surgical technique for treatment of infrabony defects with enamel matrix derivative (Emdogain): 3 case reports. Int J Periodontics Restorative Dent 1999;19:578-587.
28. Sculean A, Reich E, Chiantella GC, Brecx M. Treatment of intrabony periodontal defects with an enamel matrix protein derivative (Emdogain). a report of 32 cases. Int J Periodontics Restorative Dent 1999;19:157-163.
29. Sculean A, Donos N, Windisch P et al. Healing of human intrabony defects following treatment with enamel matrix proteins or guided tissue regeneration. J Periodont Res 1999;34:310-322.
30. Pontoriero R, Wennstrom J, Lindhe J. The use of barrier membranes and enamel matrix proteins in the treatment of angular bone defects. A prospective controlled clinical study. J Clin Periodontol 1999;26: 833-840.
31. Mellonig JT. Enamel matrix derivative for periodontal reconstructive surgery: technique and clinical and histologic case report. Int J Periodontics Restorative Dent 1999;19:8-19.
32. Hirooka H. The biologic concept for the use of enamel matrix protein: true periodontal regeneration. Quintessence Int 1998;29:621-630.
33. Heijl L, Heden G, Svardstrom G, Ostgren A. Enamel matrix derivative (EMDOGAIN) in the treatment of intrabony periodontal defects. J Clin Periodontol 1997;24:705-714.
34. Somerman MJ, Archer SY, Imm GR, Foster RA. A comparative study of human periodontal ligament cells and gingival fibroblasts in vitro. J Dent Res 1988;67:66-70.
35. Cattaneo V, Cetta G, Rota C et al. Volatile components of cigarette smoke: effect of acrolein and acetaldehyde on human gingival fibroblasts in vitro. J Periodontol 2000;71:425-432.
36. Gestrelius S, Andersson C, Lidström L, Somerman M. In vitro studies on periodontal ligament cells and enamel matrix derivative. J Clin Periodontol 1997;24:685-692.
37. Blomlöf J, Blomlöf L, Linskog S. Effect of different concentrations of EDTA on smear removal and collagen exposure in periodontitis affected root surfaces. J Clin Periodontol 1997;24:534-537.
38. H Schwarz. Two-dimensional feature shape indices. Proceedings of the 5th International Congress for Stereology, Salzburg, Austria, 1979. Mikroskopie 1980;37 Spec No: 1-495.
39. Lyngstadaas SP, Lundebrg E, Ekdahl H, Andersson C, Gestrelius S. Autocrine growth factors in human periodontal ligament cells cultured on enamel matrix derivative. J Clin Periodontol 2001;28:181-188.
40. Matsuda N, Horikawa M, Watanabe M, Kitagawa S, Kudo Y, Tarata T. Possible involvement of extracellular signal-regulated kinases 1/2 in mitogenic response of periodontal ligament cells to enamel matrix derivative. Eur J Oral Sci 2002;110: 439-444.
41. Van der Pauw MT, Van den Bos T, Everts V, Beertsen W. Enamel matrix-derived protein stimulates attachment of periodontal ligament fibroblasts and enhances alkaline phosphatase activity and transforming growth factor β1 release of periodontal ligament and gingival fibroblasts. J Periodontol 2000;71:31-43.
42. Lyngstadaas SP, Ekdahl H, Lundberg E, Andersson C, Gestrelius S. Increased TGF production in PDL cells grown on EMDODAIN® matrix. J Dent Res 1999; 78: Abstr, 671.
43. Schroeder HE. The Periodontium. Heidelberg: Springer-Verlag, 1986: 170-232.
44. Somerman MJ, Shroff B, Agraves WS et al. Expression of attachment proteins during cementogenesis. J Biol Buccale 1990;18:207-214.
45. Bronckers AL, Farach-Carson MC, Van Waveren E, Butler WT. Immunolocalization of osteopontin, osteocalcin and dentin sialoprotein during dental root formation and early cementogenesis in the rat. J Bone Miner Res 1994;9:833-841.
46. Gao J, Symons AL, Haase H, Bartold PM. Should cementoblasts express alkaline phosphatase activity? Preliminary study of rat cementoblasts in vitro. J Periodontol 1999;70:951-959.
47. Bosshardt DD, Zalzal S, McKee MD, Nanci A. Developmental appearance and distribution of bone sialoprotein and osteopontin in human rat cementum. Anat Rec 1998;250:13-33.
48. D'Errico JA, MacNeil RL, Strayhorn CL, Piotrowski BT, Somerman MJ. Models for the study of cementogenesis. Connect Tissue Res 1995;33:9-17.
49. MacNeil RL, Berry J, Strayhorn C, Somerman MJ. Expression of bone sialoprotein mRNA by cells lining the mouse tooth root during cementogenesis. Arch Oral Biol 1996;41:827-835.
50. Tenorio D, Cruchley A, Hughes FJ. Immunocytochemical investigation of the rat cementoblast phenotype. J Periodont Res 1993;28:411-419.