A single-sample method for determination of carbohydrate and protein contents glycoprotein bands separated by sodium dodecyl sulfate- polyacrylamide gel electrophoresis

Analytical Biochemistry

Volumn 275, Issue 2, 1999, Pages 171-179

  Zdebska, Ewa ^a   Kościelak, Jerzy ^a  

^a INSTITUTE OF HEMATOLOGY AND TRANSFUSION MEDICINE   (Poland)

Author keywords

Electroblotting; Glycoproteins; HPAEC; Molar composition; Polyvinylidene difluoride; Sodium dodecyl sulfate polyacrylamide gel electrophoresis

Indexed keywords

AMINO ACIDS; AMINO SUGARS; CARBOXYLIC ACIDS; CHLORINE COMPOUNDS; ELECTROPHORESIS; GLYCOPROTEINS; HYDROLYSIS; PROTEINS; SULFUR COMPOUNDS;

ELECTROBLOTTING; HPAEC; MOLAR COMPOSITIONS; POLYVINYLIDENE DIFLUORIDE; SODIUM DODECYL SULFATE-POLYACRYLAMIDE GEL ELECTROPHORESIS;

SODIUM DODECYL SULFATE;

AMINO ACID; DODECYL SULFATE SODIUM; ERYTHROCYTE BAND 3 PROTEIN; FUCOSE; GLYCOPHORIN; GLYCOPROTEIN; HEXOSAMINE; OROSOMUCOID; SIALIC ACID; SUGAR; TRANSFERRIN;

ANALYTIC METHOD; ANION EXCHANGE CHROMATOGRAPHY; ARTICLE; CARBOHYDRATE ANALYSIS; HUMAN; HYDROLYSIS; POLYACRYLAMIDE GEL ELECTROPHORESIS; PRIORITY JOURNAL; PROTEIN ANALYSIS; PROTEIN CONTENT; PROTEIN DEGRADATION; PROTEIN MODIFICATION;

EID: 0345148486     PISSN: 00032697     EISSN: None     Source Type: Journal    
DOI: 10.1006/abio.1999.4294     Document Type: Article

References (41)

1. Sears P., Wong C. H. Enzyme action in glycoprotein synthesis. Cell Mol. Life Sci. 54:1998;223-252.
2. Hart G. W. Dynamic O-linked glycosylation of nuclear and cytoskeletal glycoproteins. Annu. Rev. Biochem. 66:1997;315-335.
3. Rademacher T. W., Parekh R. B., Dwek R. A. Glycobiology. Annu. Rev. Biochem. 57:1988;785-838.
4. Kościelak J. A hypothesis on the biological role of ABH, Lewis and P blood group determinant structures in glycosphingolipids and glycoproteins. Glycoconj. J. 3:1986;95-108.
5. Karlsson K-A. Microbial recognition of target cell glycoconjugates. Curr. Opin. Struct. Biol. 5:1995;622-635.
6. Kościelak J. Diseases of aberrant glycosylation. Acta Biochim. Pol. 42:1995;1-10.
7. Kamemura K., Kato S. Detections of lectins using ligand blotting and polyacrylamide-type glycoconjugate probes. Anal. Biochem. 258:1998;305-310.
8. Bischoff K. M., Shi L., Kennelly P. J. The detection of enzyme activity following sodium dodecyl sulfate-polyacrylamide gel electrophoresis. Anal. Biochem. 260:1998;1-17.
9. Gultekin H., Heermann K. H. The use of polyvinylidene difluoride membranes as a general blotting matrix. Anal. Biochem. 172:1988;320-329.
10. Ogawa H., Ueno H., Uchibori S., Matsumoto J., Seno N. Direct carbohydrate analysis of glycoproteins electroblotted onto polyvinylidene difluoride membrane from sodium dodecyl sulfate-polyacrylamide gel electrophoresis. Anal. Biochem. 190:1990;165-169.
11. Weitzhandler M., Kadlecek D., Avdalovic N., Forte J. G., Chow D., Townsend R. R. Monosaccharide and oligosaccharide analysis of proteins transferred to polyvinyldifluoride membranes after sodium dodecyl sulfate-polyacrylamide gel electrophoresis. J. Biol. Chem. 268:1993;5121-5130.
12. Valenzano K. J., Kallay L. M., Lobel P. An assay to detect glycoproteins that contain mannose 6-phosphate. Anal. Biochem. 209:1993;156-162.
13. Zhu Q., Bhavanandan V. P. Analysis of serine/threonine-linked oligosaccharides derived by alkaline-borohydride treatment of mucin glycoprotein electroblotted onto membranes: Comparison of the saccharide profiles of the 390 kDa and 350 kDa forms of epitectin. Glycoconj. J. 12:1995;639-644.
14. Duk M., Ugorski M., Lisowska E. β-Elimination of O-glycans from glycoproteins transferred to immobilon membranes: Method and some applications. Anal. Biochem. 253:1997;98-102.
15. Papac D. J., Briggs J. B., Chiin E. T., Jones A. J. A high-throughput microscale method to release N-linked oligosaccharides from glycoproteins for matrix-assisted laser desorption/ionization. Glycobiology. 8:1998;445-454.
16. Nakagawa S., Fukuda T. Direct amino acid analysis of proteins electroblotted onto polyvinylidene difluoride membrane from sodium dodecyl sulfate-polyacrylamide gel. Anal. Biochem. 181:1989;75-78.
17. Tous G. I., Fausnaugh J. L., Akinyosoye O., Lackland H., Winter-Cash P., Vitorica F. J., Stein S. Amino acid analysis on polyvinylidene difluoride membranes. Anal. Biochem. 179:1989;50-55.
18. Matsudaira P. Sequence from picomole quantities of proteins electroblotted onto polyvinylidene difluoride membranes. J. Biol. Chem. 262:1987;10035-10038.
19. Yamashita K., Ideo H., Ohkura T., Fukushima K., Yuasa I., Onho K., Takeshita K. Sugar chains of serum transferrin from patients with carbohydrate deficient glycoprotein syndrome. Evidence of asparagine-N-linked oligosaccharide transfer deficiency. J. Biol. Chem. 268:1993;5784-5789.
20. Fukuda M., Eshdat Y., Tarone G., Marchesi V. T. Isolation and characterization of peptides derived from the cytoplasmic segment of band 3, the predominant intrinsic membrane protein of the human erythrocyte. J. Biol. Chem. 253:1978;2419-2428.
21. Tyler J. M., Hargreaves W. R., Branton D. Purification of two spectrin-binding proteins: Biochemical and electron microscopic evidence for site-specific reassociation between spectrin and band 2.1 and 4.1. Proc. Natl. Acad. Sci. USA. 76:1979;5192-5196.
22. Laemmli U. K. Cleavage of structural proteins during the assembly of the head of bacteriophage T. Nature. 227:1970;680-685.
23. Towbin H., Stachelin T., Gordon H. Electrophoretic transfer of proteins from polyacrylamide gels to nitrocellulose sheets procedure and some application. Proc. Natl. Acad. Sci. USA. 76:1979;4350-4354.
24. Merkle R. K., Poppe I. Lennarz W. J., Hart G. W. Methods in Enzymology, Vol. 230. Guide to Techniques in Glycobiology. 1994;1-15 Academic Press, San Diego.
25. Undefriend S., Stein S., Böhlen P., Dairman W., Leimbgruber W., Weigele M. Fluorescamine: A reagent for assay of amino acids, peptides, proteins, and primary amines in the picomole range. Science. 178:1972;871-872.
26. Yemm E. W., Cocking E. C. The determination of amino acids with ninhydrin. Analyst. 80:1955;207-213.
27. Benson J. R., Hare P. E. O-Phthalalaldehyde: Fluorogenic detection of primary amines in the picomole range. Comparison with fluorescamine and ninhydrin. Proc. Natl. Acad. Sci. USA. 72:1975;619-622.
28. Tanner M. J. A. Molecular and cellular biology of the erythrocyte anion exchanger (AE1). Semin. Hematol. 30:1993;34-57.
29. MacGillivray R. T. A., Mendez E., Shevale J. G., Sinha S. K., Lineback-Zins J., Brew K. The primary structure of human serum transferrin. The structures of seven cyanogen bromide fragments and the assembly of the complete structure. J. Biol. Chem. 258:1983;3543-3553.
30. Spik G., Debruyne V., Montreuil J., van Halbeek H., Vliegenthart J. F. G. Primary structure of two sialylated triantennary glycans from human serotransferrin. FEBS Lett. 183:1985;65-69.
31. 1-Acid Glycoprotein Genetics, Biochemistry, Physiological Functions, and Pharmacology. 1989;7-22 A. R. Liss, New York.
32. Pisano A., Redmond J. W., Williams K. R., Gooley A. A. Glycosylation sites identified by solid-phase Edman degradation: O-linked glycosylation motifs on human glycophorin A. Glycobiology. 3:1993;429-435.
33. Conboy J. G. Structure, function, and molecular genetics of erythroid membrane skeletal protein 4.1 in normal and abnormal red blood cells. Semin. Hematol. 30:1993;58-73.
34. Stibler H., Borg S., Allgulander C. Clinical significance of abnormal heterogeneity of transferrin in relation to alcohol consumption. Acta Med. Scand. 206:1979;275-281.
35. Landberg E., Pahlsson P., Lundblad A., Arnetorp A., Jeppsson J-O. Carbohydrate composition of serum transferrin isoforms from patients with high alcohol consumption. Biochem. Biophys. Res. Commun. 210:1995;267-274.
36. Coddeville B., Carchon H., Jaeken J., Briand G., Spik G. Determination of glycan structure and molecular masses of the glycovariants of serum transferrin from a patient with carbohydrate-deficient syndrome type II. Glycoconj. J. 15:1998;265-273.
37. Watkins W. M. Montreuil J., Vliegenthart J. F. G., Schachter H. Glycoproteins. 1995;313-390 Elsevier Science B. V. Amsterdam.
38. Anstee D. J. Blood group-active surface. Molecules of the human red blood cell. Vox Sang. 58:1990;1-20.
39. Mawby W. J., Tanner M. J. A., Anstee D. J., Clamp J. R. Incomplete glycosylation of erythrocyte membrane proteins in congenital dyserythropoietic anemia type II (CDA II). Br. J. Haematol. 55:1983;357-368.
40. 1-acid glycoprotein. Carbohydr. Res. 46:1976;75-86.
41. Holt G. D., Haltiwanger R. S., Torres C. R., Hart G. W. Erythrocytes contain cytoplasmic glycoproteins, O-linked GlcNAc on band 4.1. J. Biol. Chem. 262:1987;14847-14850.