Mutations abrogating the RNase activity in glycoprotein E(rns) of the pestivirus classical swine fever virus lead to virus attenuation

Journal of Virology

Volumn 73, Issue 12, 1999, Pages 10224-10235

  Meyers, Gregor ^a   Saalmüller, Armin ^a   Büttner, Mathias ^a  

^a FEDERAL RESEARCH INSTITUTE FOR ANIMAL HEALTH   (Germany)

Author keywords

[No Author keywords available]

Indexed keywords

GLYCOPROTEIN; HISTIDINE; RIBONUCLEASE;

ARTICLE; BLEEDING DISORDER; CODON; CONTROLLED STUDY; ENZYME ACTIVITY; FEVER; NONHUMAN; OPEN READING FRAME; PESTIVIRUS; PRIORITY JOURNAL; SITE DIRECTED MUTAGENESIS; VIREMIA; VIRUS ATTENUATION;

EID: 0345148344     PISSN: 0022538X     EISSN: None     Source Type: Journal    
DOI: 10.1128/jvi.73.12.10224-10235.1999     Document Type: Article

References (41)

1. Baker, J. C. 1987. Bovine viral diarrhea virus: a review. J. Am. Vet. Med. Assoc. 190:1449-1458.
2. Benner, S. A., and R. K. Allemann. 1989. The return of pancreatic ribonucleases. Trends Biochem. Sci. 14:395-397.
3. rns of pestiviruses induces apoptosis in lymphocytes of several species. J. Virol. 71:6692-6696.
4. D'Alessio, G. 1993. New and cryptic biological messages from RNases. Trends Cell Biol. 3:106-109.
5. D'Alessio, G., A Di Donato, A. Parente, and R. Picoli. 1991. Seminal RNase: a unique member of the ribonuclease superfamily. Trends Biochem. Sci. 16:104-106.
6. Denham, S., R. J. Zwart, J. T. D. Whittall, M. Pambusch, A. H. Ccorteyn, A. T. J. Bianchi, M. P. Murtaugh, R. M. E. Parkhouse, H. Tlaskalova, J. Sinkora, M. Sinkora, and Z. Rehakova. 1998. Monoclonal antibodies putatively identifying porcine B cells. Vet. Immunol. Immunopathol. 60:317-328.
7. Donis, R. O., W. Corapi, and E. J. Dubovi. 1988. Neutralizing monoclonal antibodies to bovine viral diarrhea virus bind to the 56K to 58K glycoprotein. J. Gen. Virol. 69:77-86.
8. Fuerst, T. R., E. G. Niles, F. W. Studier, and B. Moss. 1986. Eukaryotic transient expression system based on recombinant vaccinia virus that synthesizes bacteriophage T7 RNA polymerase. Proc. Natl. Acad. Sci. USA 83:8122-8126.
9. Hulst, M. M., G. Himes, E. Newbigin, and R. J. M. Moormann. 1994. Glycoprotein E2 of classical swine fever virus: expression in insect cells and identification as a ribonuclease. Virology 200:558-565.
10. rns of classical swine fever virus results in a cytopathogenic virus. J. Virol. 72:151-157.
11. Kessler, S. W. 1981. Use of protein A-bearing staphylococci for the immunoprecipitation and isolation of antigens from cells. Methods Enzymol. 73: 442-459.
12. König, M. 1994. Virus der klassischen Schweinepest: Untersuchungen zur Pathogenese und zur Induktion einer protektiven Immunantwort. Ph.D. thesis. Veterinary School of Hannover, Hannover, Germany.
13. Kunkel, T. A., J. D. Roberts, and R. A Zakour. 1987. Rapid and efficient site-specific mutagenesis without phenotypic selection. Methods Enzymol. 154:367-392.
14. Meyers, G., T. Rümenapf, and H.-J. Thiel. 1989. Molecular cloning and nucleotide sequence of the genome of hog cholera virus. Virology 171:555-567.
15. Meyers, G., N. Tautz, P. Becher, H.-J. Thiel, and B. M. Kümmerer. 1996 Recovery of cytopathogenic and noncytopathogenic bovine viral diarrhea viruses from cDNA constructs. J. Virol. 70:8606-8613.
16. Meyers, G., H.-J. Thiel, and T. Rümenapf. 1996. Classical swine fever virus: recovery of infectious viruses from cDNA constructs and generation of recombinant cytopathogenic swine fever virus. J. Virol. 67:7088-7095.
17. Meyers, G. Unpublished results.
18. Moennig, V., and J. Plagemann. 1992. The pestiviruses. Adv. Virus Res. 41:53-91.
19. Paton, D. J., J. P. Lowings, and A. D. Barrett. 1992. Epitope mapping of the gp53 envelope protein of bovine viral diarrhea virus. Virology 190:763-772.
20. Pesvovitz, M. D., J. K. Lunney, and D. H. Sachs. 1984. Preparation and characterization of monoclonal antibodies reactive with porcine PBL. J. Immunol. 133:368-375.
21. Rice, C. M. 1996. Flaviviridae: the viruses and their replication, p. 931-959. In B. N. Fields, D. M. Knipe, and P. M. Howley, (ed.), Fields virology. Lippincott-Raven, Philadelphia, Pa.
22. Rümenapf, T., G. Meyers, R. Stark, and H.-J. Thiel. 1989. Hog cholera virus characterization of specific antiserum and identification of cDNA clones. Virology 171:18-27.
23. Rümenapf, T., G. Unger, J. H. Strauss, and H.-J. Thiel. 1993. Processing of the envelope glycoproteins of pestiviruses. J. Virol. 67:3288-3294.
24. + cytolytic T lymphocytes by the expression of CD5 antigen. Immunology 81:578-583.
25. Saalmüller, A., M. J. Reddehase, H.-J. Bühring, S. Jonjic, and U. H. Kozinowski. 1987. Simultaneous expression of CD4 and CD8 antigens by a substantial proportion of resting porcine T lymphocytes. Eur. J. Immunol. 17:1297-1301.
26. Saalmüller, A. Unpublished results.
27. Sambrook, S., E. F. Fritsch, and T. Maniatis. 1989. Molecular cloning: a laboratory manual, 2nd ed. Cold Spring Harbor Laboratory Press, Cold Spring Harbor, N.Y.
28. Schneider, R., G. Unger, R. Stark, E. Schneider-Scherzer, and H.-J. Thiel. 1993. Identification of a structural glycoprotein of an RNA virus as a ribonuclease. Science 261:1169-1171.
29. Sopp, P., L. Redknap, and C. Howard. 1998. Cross-reactivity of human leucocyte differentiation antigen monoclonal antibodies on porcine cells. Vet. Immunol. Immunopathol. 60:403-408.
30. Susa, M., M. König, A. Saalmüller, M. J. Reddehase, and H. J. Thiel. 1992. Pathogenesis of classical swine fever: B-lymphocyte deficiency caused by hog cholera virus. J. Virol. 66:1171-1175.
31. Tautz, N., H.-J. Thiel, E. Dubovi, and G. Meyers. 1994. Pathogenesis of mucosal disease: a cytopathogenic pestivirus generated by an internal deletion. J. Virol. 68:3289-3297.
32. Thiel, H.-J., G. W. Plagemann, and V. Moennig. 1996. The pestiviruses, p. 1059-1073. In B. N. Fields, D. M. Knipe, and P. M. Howley (ed.), Fields virology. Lippincott-Raven, Philadelphia, Pa.
33. Thiel, H.-J., R. Stark, E. Welland, T. Rümenapf, and G. Meyers. 1991. Hog cholera virus: molecular composition of virions from a pestivirus. J. Virol. 65:4705-4712.
34. Tratschin, J. D., C. Moser, N. Ruggli, and M. A. Hofmann. 1998. Classical swine fever virus leader proteinase Npro is not required for viral replication in cell culture. J. Virol. 72:7681-7684.
35. Van der Werf, S., J. Bradley, E. Wimmer, F. W. Studier, and J. J. Dunn. 1986. Synthesis of infectious poliovirus RNA by purified T7 RNA polymerase. Proc. Natl. Acad. Sci. USA 83:2330-2334.
36. van Rijn, P. A., H. G. van Gennip, E. J. de Meijer, and R. J. Moormann. 1993. Epitope mapping of envelope glycoprotein E1 of hog cholera virus strain Brescia. J. Gen. Virol. 74:2053-2060.
37. Weiland, E., R. Ahl, R. Stark, F. Weiland, and H.-J. Thiel. 1992. A second envelope glycoprotein mediates neutralization of a pestivirus, hog cholera virus. J. Virol. 66:3677-3682.
38. Weiland, E., R. Stark, B. Haas, T. Rümenapf, G. Meyers, and H.-J. Thiel. 1990. Pestivirus glycoprotein which induces neutralizing antibodies forms part of a disulfide-linked heterodimer. J. Virol. 64:3563-3569.
39. Weiland, E., H.-J. Thiel, G. Hess, and F. Weiland. 1989. Development of monoclonal neutralizing antibodies against bovine viral diarrhea virus after pretreatment of mice with normal bovine cells and cyclophosphamide. J. Virol. Methods 24:237-244.
40. Windisch, J. M., R. Schneider, R. Stark, E. Weiland, G. Meyers, and H.-J. Thiel. 1996. RNase of classical swine fever virus: biochemical characterization and inhibition by virus-neutralizing monoclonal antibodies. J. Virol. 70:352-358.
41. Wu, Y. N., S. M. Mikulski, W. Ardelt, S. M. Rybak, and R. J. Youle. 1993. A cytotoxic ribonuclease. J. Biol. Chem. 268:10686-10693.