Purification, N-terminal sequencing and diagnostic value of the major antigens of Ornithodoros erraticus and O. moubata

Veterinary Parasitology

Volumn 87, Issue 2-3, 2000, Pages 193-206

  Baranda, José A ^a   Pérez Sánchez, Ricardo ^a   Oleaga, Ana ^b   Manzano, Raúl ^a   Encinas Grandes, Antonio ^a  

^a Lab Parasitol Fac Farmacia U   (Spain)

^b U Patol Anim Inst Recurs N   (Spain)

Author keywords

Antigens; Characterization; Ornithodoros; Serology

Indexed keywords

ANIMAL MODEL; ANIMAL TISSUE; ANTIGEN PURIFICATION; ARTICLE; CONTROLLED STUDY; ENZYME LINKED IMMUNOSORBENT ASSAY; HIGH PERFORMANCE LIQUID CHROMATOGRAPHY; NONHUMAN; POLYACRYLAMIDE GEL ELECTROPHORESIS; PROTEIN ANALYSIS; SEQUENCE ANALYSIS; SERODIAGNOSIS; SWINE; TICK;

AMINO ACID SEQUENCE; ANIMALS; ANTIBODIES; ANTIGENS; CHROMATOGRAPHY, HIGH PRESSURE LIQUID; CHROMATOGRAPHY, ION EXCHANGE; ENZYME-LINKED IMMUNOSORBENT ASSAY; FEMALE; MALE; MOLECULAR SEQUENCE DATA; RABBITS; SENSITIVITY AND SPECIFICITY; SEQUENCE ANALYSIS, PROTEIN; SEQUENCE HOMOLOGY, AMINO ACID; SWINE; SWINE DISEASES; TICK INFESTATIONS; TICKS;

MAMMALIA; ORNITHODOROS MOUBATA; ORYCTOLAGUS CUNICULUS; SUS SCROFA;

EID: 0345061659     PISSN: 03044017     EISSN: None     Source Type: Journal    
DOI: 10.1016/S0304-4017(99)00169-7     Document Type: Article

References (16)

1. Astigarraga A., Oleaga-Pérez A., Pérez-Sánchez R., Baranda J.A., Encinas-Grandes A. Host immune response evasion strategies in Ornithodoros erraticus and O. moubata and their relationship to the development of an antiargasid vaccine. Parasite Immunol. 19:1997;401-410.
2. Bachmann M.F., Zinkernagel R. The influence of virus structure on antibody responses and virus serotype formation. Immunol. Today. 17:1996;553-558.
3. Baranda J.A., Pérez-Sánchez R., Oleaga-Pérez A., Encinas-Grandes A. Antigens of interest for the diagnosis of parasitism in pigs by Ornithodoros erraticus and O. moubata. J. Parasitol. 83:1997;831-838.
4. Bradford M.M. A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein dye binding. An. Biochem. 72:1976;248-254.
5. Canals A., Oleaga A., Pérez R., Domínguez J., Encinas A., Sánchez-Vizcaino J.M. Evaluation of an enzyme-linked immusorbent assay to detect specific antibodies in pigs experimental and field infected with Ornithodoros erraticus. Vet. Parasitol. 37:1990;145-153.
6. Coligan, J.E., Dunn, B.M., Ploegh, H.L., Speicher, D.W., Wingfield, P.T. (Ed.), 1997. Current Protocols in Protein Science,vol 1. Wiley .
7. Gabaj M.M., Awan M.A.Q., Beesley W.N. A survey of ticks on farm animals in Libya. Ann. Trop. Med. Parasitol. 86:1992;543-548.
8. Harlow, E., Lane, D. (Ed.), 1988. Antibodies: A Laboratory Manual. Cold Spring Harbor Laboratory, New York, pp. 511-551.
9. Laemmli U.K. Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature. 227:1970;680-681.
10. Logan T.M., Wilson M.L., Cornet J.P. Association of ticks (Acari: Ixodoidea) with rodent burrows in northern Senegal. J. Med. Entomol. 30:1993;799-801.
11. Markwell M.K., Hass S.M., Bieber L.L., Tolbert N.E. A modification of the Lowry procedure to simplify protein determination in membrane and lipoprotein samples. An. Biochem. 87:1978;206-210.
12. Mond J.J., Lees A., Snapper C.M. T cell-independent antigens type 2. Annu. Rev. Immunol. 13:1995;655-692.
13. Oleaga-Pérez A., Pérez-Sánchez R., Encinas-Grandes A. Distribution and biology of Ornithodoros erraticus in parts of Spain affected by African swine fever. Vet. Rec. 126:1990;32-37.
14. Oleaga-Pérez A., Pérez-Sánchez R., Astigarraga A., Encinas-Grandes A. Detection of pig farms with Ornithodoros erraticus by pig serology. Elimination of non-specific reactions by carbohydrate epitopes of salivary antigens. Vet. Parasitol. 52:1994;97-111.
15. Pérez-Sánchez R., Oleaga-Pérez A., Encinas-Grandes A. Analysis of the specificity of the salivary antigens of Ornithodoros erraticus for the purpose of serological detection of swine farms harbouring the parasite. Parasite Immunol. 14:1992;201-216.
16. Pérez-Sánchez R., Astigarraga A., Oleaga-Pérez A., Encinas-Grandes A. Relationship between the persistence of African swine fever and the distribution of Ornithodoros erraticus in the province of Salamanca, Spain. Vet. Rec. 135:1994;207-209.