Detection of Dioxygenase Genes Present in Various Activated Sludge

Environmental Science and Pollution Research

Volumn 10, Issue 6, 2003, Pages 373-378

  Moharikar, Aditi ^a   Kapley, Atya ^a   Purohit, Hemant J ^a  

^a NATIONAL ENVIRONMENTAL ENGINEERING RESEARCH INSTITUTE   (India)

Author keywords

16S rDNA; Activated sludge; Aromatics; Biodegradation; Chemical oxygen demand (COD); Dioxygenases; Diversity; Effluent treatment; Effluent treatment plants (ETPs); Mother liquor suspended solids (MLSS) analysis; Polymerase chain reaction (PCR); Tracking

Indexed keywords

ACTIVATED SLUDGE;

BACTERIA (MICROORGANISMS); GADUS MORHUA;

EID: 0345015478     PISSN: 09441344     EISSN: None     Source Type: Journal    
DOI: 10.1065/espr2003.07.164     Document Type: Article

References (26)

1. Amann R, Ludwig W, Schleifer K (1995): Phylogenetic identification and in situ detection of individual microbial cells without cultivation. Microbiol Rev 59, 143-169
2. American Public Health Association, American Water Works Association, and Water Pollution Control Federation, 1998. Standard Methods for the examination of water and wastewater 20th edition, Washington D.C.
3. Atuanya E, Purohit H J, Chakrabarti T (2000): Anaerobic and aerobic biodegradation of chlorophenols using UASB and ASG bioreactors. World J Microbiol & Biotechnol 16, 95-98
4. Chattre S, Purohit H J, Shanker R, Khanna P (1996): Bacterial consortia for crude oil spill remediation. Wat Sci Tech 34, 187-193
5. Harayama S, Kok M, Neidle E (1992): Functional and evolutionary relationships among diverse oxygenases. Annu Rev Microbiol 46, 565-601
6. Holben W, Jannson J, Chelm B, Tiedje J (1988): DNA probe method for the detection of specific microorganisms in the soil bacterial community. Appl Environ Microbiol 54, 703-711
7. Holben W, Schroeter B, Calabrese V, Olsen R, Kukor J, Biederbeck V, Smith A, Tiedje J (1992): Gene probe analysis of soil microbial populations selected by amendment with 2,4-dichlorophenoxyacetic acid. Appl Environ Microbiol 58, 3941-3948
8. Ka J, Holben W, Tiedje J (1994): Use of gene probes to aid in recovery and identification of functionally dominant 2,4-dichlorophenoxyacetic acid-degrading populations in soil. Appl Environ Microbiol 60, 1116-1120
9. Kapley A, Lampel K, Purohit H J (2000): Development of duplex PCR for the detection of Salmonella and Vibrio in drinking water. World J Microbiol & Biotech 16, 457-458
10. Kapley A, Purohit H J (2001): Tracking of phenol degrading genotype. ESPR - Environ Sci & Pollu Res 8, 89-90
11. Kapley A, Purohit H J, Chhatre S, Shanker R, Chakrabarti T, Khanna P (1999): Osmotolerance and hydrocarbon degradation by genetically engineered bacterial consortium. Bio-Resource Technology 67, 241-245
12. Kivisaar M, Horak R, Kasak L, Heinaru A, Habieht J (1990): Selection of independent plasmids determining phenol degradation in Pseudomonas putida and the cloning and expression of genes encoding phenolmonooxygenase and catechol 1,2-dioxygenase. Plasmid 24, 25-36
13. Kutty R, Purohit H J, Khanna P (2000): Isolation and characterization of Pseudomonas sp. strain PH1 utilizing meta-aminophenol. Can J Microbiol 46, 211-217
14. Lee J, Jung K, Choi S, Kim H (1995): Combination of the tod and the tol pathways in redesigning a metabolic route of Pseudomonas putida for the mineralization of a benzene, toluene, and p-xylene mixture. Appl Environ Microbiol 61, 2211-2217
15. Lee S, Fuhrman J (1989): DNA hybridization to compare species compositions of natural bacterioplankton assemblages. Appl Environ Microbiol 56, 739-746
16. Maniatis T, Fritsch E, Sambrook J (1989): Molecular cloning: a laboratory manual. Cold Spring Harbor Laboratory, Cold Spring Harbor, N.Y. 2nd edition
17. McClure N, Fry J, Weightman A (1991): Survival and catabolic activity of natural and genetically engineered bacteria in a laboratory-scale activated sludge unit. Appl Environ Microbiol 57, 366-373
18. Picard C, Ponsonnet C, Paget E, Nesme X and Simonet P (1992): Detection and enumeration of bacteria in soil by direct DNA extraction and polymerase chain reaction. Appl Environ Microbiol 58, 2717-2722
19. Purohit H J, Kapley A, Moharikar A, Narde G (2002): A novel approach for extraction of PCR-compatible DNA from activated sludge samples collected from different biological effluent treatment plants. J Microbiol Meth 52, 315-323
20. Purohit H J, Chhatre S, Kapley A, Khanna P (1997): Microbial consortia: An approach for management of oil-spill pollution. ENFO News. March, 2-3
21. Qureshi A, Prabu S, Purohit H J (2001): Isolation and characterization of Pseudomonas strains for utilization of p-nitrophenol. Microbes & Environ 16, 49-52
22. Qureshi A, Purohit H J (2002): Isolation of bacterial consortium for degradation of p-nitrophenol from agricultural soil. Annals of Appl Biol 140, 159-162
23. Sayler G, Layton A (1990): Environmental application of nucleic acid hybridization. Annu Rev Microbiol 44, 625-648
24. Spain J, Zylstra G, Blake C, Gibson D (1989): Monohydroxylation of phenol and 2,5-dichlorophenol by toluene dioxygenase in Pseudomonas putida F1. Appl Environ Microbiol 55, 2648-2652
25. Widada J, Nojiri H, Kasuga K, Yoshida T, Habe H, Omori T (2002): Molecular detection and diversity of polycyclic aromatic hydrocarbon-degrading bacteria isolated from geographically diverse sites. Appl Microbiol Biotechnol 58, 202-209
26. Yeates C, Gillings M, Davison A, Altavilla N and Veal D (1997): PCR amplification of crude microbial DNA extracted from soil. Lett in Appl Microbiol 25, 303-307