A method for evaluating lactose hydrolysis in a fixed bed reactor with β-galactosidase immobilized on chitosan

Chemical Engineering Journal

Volumn 65, Issue 2, 1997, Pages 93-98

  Carrara, Carlos R ^a   Rubiolo, Amelia C ^a  

^a UNIVERSIDAD NACIONAL DEL LITORAL   (Argentina)

Author keywords

Lactose; Model; Reactor; Galactosidase

Indexed keywords

ENZYME IMMOBILIZATION; HYDROLYSIS; MATHEMATICAL MODELS; REACTION KINETICS;

CHITOSAN; FIXED BED REACTOR; GALACTOSIDASE; LACTOSE;

PACKED BEDS;

EID: 0344878873     PISSN: 13858947     EISSN: None     Source Type: Journal    
DOI: 10.1016/S1385-8947(96)03161-0     Document Type: Article

References (14)

1. M. Lilly and P. Dunnill, Immobilized-enzymes reactors, Methods Enzymol. Immobil. Enzym., 44 (1976) 717-738.
2. V.S. Patwradhan and N.G. Karanth, Film diffusional influences on the kinetic parameters in packed-bed immobilized enzyme reactors, Biotechnol. Bioeng., 24 (1982) 763-780.
3. R. Lortie and D. Thomas, Heterogeneous one-dimensional model for fixed bed enzyme reactors, Biotechnol. Bioeng., 28 (1986) 1256-1260.
4. C.R. Carrara and A.C. Rubiolo, The immobilization of β-galactosidase on chitosan, Biotechnol. Prog., 10 (1994) 220-224.
5. R. Mahoney and J. Whitaker, Stability and enzymatic properties of β-Galactosidase from Kluyveromices fragilis, J. Food Biochem., 1 (1977) 327-350.
6. J. Braun, P. Le Chanu and F. Le Goffic, The immobilization of Penicillun G-acylase on chitosan, Biotechnol. Bioeng., 33 (1989) 242-246.
7. J.M. Engasser and C. Horvath, Diffusion and kinetics with immobilized enzymes, Appl. Biochem. Bioeng., 1 (1976) 128-328.
8. J. Park and C. Choi, Effect of mass transfer in a recirculation batch reactor system for immobilized Penicillun Amidase, Biotechnol. Bioeng., 24 (1982) 2215-2226.
9. C.J. Geankoplis, Transport Processes and Unit Operation, 2nd edn., Allyn and Bacon, Massachusetts, 1978.
10. S. Henley and T. Sadana, Deactivation theory, Biotechnol. Bioeng., 28 (1986) 1277-1285.
11. C.R. Carrara and A.C. Rubiolo, Determination of kinetics parameters for free and immobilized β-galactosidase, Process Biochem., 31 (1996) 243-248.
12. R. Weast and A. Melvin, Handbook of Chemistry and Physics, 69th edn., CRC Press, Boca Raton, FL, 1989.
13. R. Perry and C. Chilton, Chemical Engineer's Handbook, 6th edn., MacGraw-Hill, New York, 1984.
14. J. Prenosil, O. Kut, I. Dunn and E. Henzle, Immobilized biocatalyst, in W. Gerhartz and Y.Z. Yamamoto (eds.), Ullmann's Encyclopedia of Industrial Chemistry, Vol. A14, 5th edn., VCH, Weinhein, 1989.