Role of cysteine amino acid residues on the RNA binding activity of human thymidylate synthase

Nucleic Acids Research

Volumn 31, Issue 16, 2003, Pages 4882-4887

  Lin, Xiukun ^b   Liu, Jun ^b   Maley, Frank ^a   Chu, Edward ^b,c  

^a WADSWORTH CENTER   (United States)

^b YALE CANCER CENTER   (United States)

^c VA CONNECTICUT HEALTHCARE SYSTEM   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

ALANINE; AMINO ACID; CYSTEINE; LUCIFERASE; MESSENGER RNA; MUTANT PROTEIN; RNA; THYMIDYLATE SYNTHASE;

ARTICLE; CANCER CELL CULTURE; COLON CANCER; CONTROLLED STUDY; ENZYME ACTIVITY; ENZYME INACTIVATION; ENZYME REGULATION; GENE MUTATION; GENE REPRESSION; GENETIC TRANSFECTION; HUMAN; HUMAN CELL; IN VITRO STUDY; IN VIVO STUDY; PRIORITY JOURNAL; PROTEIN EXPRESSION; REPORTER GENE; RNA BINDING; RNA TRANSLATION; WILD TYPE;

ALANINE; CELL LINE, TUMOR; CYSTEINE; ELECTROPHORETIC MOBILITY SHIFT ASSAY; HUMANS; LUCIFERASES; MUTAGENESIS, SITE-DIRECTED; MUTATION; PROTEIN BINDING; PROTEIN BIOSYNTHESIS; RNA, MESSENGER; RNA-BINDING PROTEINS; THYMIDYLATE SYNTHASE; TRANSFECTION;

EID: 0344875508     PISSN: 03051048     EISSN: None     Source Type: Journal    
DOI: 10.1093/nar/gkg678     Document Type: Article

References (32)

1. Hardy,L.W., Finer-Moore,J.S., Montfort,W.R., Jones,M.O., Santi,D.V. and Stroud,R.M. (1987) Atomic structure of thymidylate synthase: target for rational drug design. Science, 235, 448-455.
2. Danenberg,P.V. (1977) Thymidylate synthase: a target enzyme in cancer chemotherapy. Biochim. Biophys Acta, 473, 73-79.
3. Carreras,C. and Santi,D.V. (1995) The catalytic mechanism and structure of thymidylate synthase. Annu. Rev. Biochem., 64, 721-762.
4. Chu,E., Koeller,D.M., Casey,J.L., Drake,J.C., Chabner,B.A., Elwood,P.C., Zinn,S. and Allegra,C.J. (1991) Autoregulation of human thymidylate synthase messenger RNA translation by thymidylate synthase. Proc. Natl Acad. Sci. USA, 88, 8977-8981.
5. Chu,E., Voeller,D., Koeller,D.M., Drake,J.C., Takimoto,C.H., Maley,G.F., Maley,F. and Allegra,C.J. (1993) Identification of an RNA binding site for human thymidylate synthase. Proc. Natl Acad. Sci. USA, 90, 517-521.
6. Chu,E., Voeller,D.M., Jones,K.L., Takechi,T., Maley,G.F., Maley,F., Segal,S. and Allegra,C.J. (1994) Identification of a thymidylate synthase ribonucleoprotein complex in human colon cancer cells. Mol. Cell. Biol., 14, 207-213.
7. Voeller,D.M., Changchien,L.M., Maley,G.F., Maley,F., Takechi,T., Turner,R.E., Montfort,W.R., Allegra,C.J. and Chu,E. (1995) Characterization of a specific interaction between Escherichia coli thymidylate synthase and Escherichia coli thymidylate synthase. Nucleic Acids Res., 23, 869-875.
8. Chu,E. and Allegra,C.J. (1996) The role of thymidylate synthase as an RNA binding protein. BioEssays, 18, 191-198.
9. Lin,X., Voeller,D.M., Allegra,C.J., Maley,F. and Chu,E. (2000) Characterization of a cis-acting regulatory element in the protein coding region of thymidylate synthase mRNA. Nucleic Acids Res., 28, 1381-1389.
10. Plese,P.C. and Dunlap,R.B. (1977) Sulfhydryl group modification of thymidylate synthetase and its effect on activity and ternary complex formation. J. Biol. Chem., 252, 6139-6144.
11. Chu,E., Voeller,D.M., Morrison,P.F., Jones,K.L., Takechi,T., Maley,G.F., Maley,M.F. and Allegra,C.J. (1994) The effect of reducing reagents on binding of thymidylate synthase protein to thymidylate synthase messenger RNA. J. Biol. Chem., 269, 20289-20293.
12. Hoganson,D.K., Williams,A.W. and Berger,S.H. (1999) Isolation and characterization of a thymidylate synthase-deficient human colon tumor cell line. Biochem. Pharmacol., 58, 1529-1537.
13. Ju,J.F., Pedersen-lane,J., Maley F. and Chu,E. (1999) Regulation of p53 expression by thymidylate synthase. Proc. Natl Acad. Sci. USA, 96, 3769-3774.
14. Pedersen-Lane,J., Maley,G.F., Chu,E. and Maley,F. (1997) High-level expression of human thymidylate synthase. Protein Expr. Purif., 10, 256-262.
15. Liebold,E. and Munro,H.N. (1988) Structural requirements of iron-responsive elements for binding of the protein involved in both transferrin receptor and ferritin mRNA post-transcriptional regulation. Proc. Natl Acad. Sci. USA, 85, 2171-2175.
16. Laemmli,U.K. (1970) Cleavage of structural proteins during the assembly of the head of bacteriphage T4. Nature, 227, 680-685.
17. Felgner,P.L., Gadek,T.R., Holm,M., Roman,R., Chan,H.W., Wenz,M., Northrop,J.P., Ringold,G.M. and Danielsen,M. (1987) Lipofection: a highly efficient, lipid-mediated DNA-transfection procedure. Proc. Natl Acad. Sci. USA, 84, 7413-7417.
18. Lin,X., Mizunuma,N., Chen,T.M., Copur,S.M., Maley,G.F., Liu,J., Maley,F. and Chu E. (2000) In vitro selection of an RNA sequence that interacts with high affinity with thymidylate synthase. Nucleic Acids Res., 28, 1381-1389.
19. Chu,E., Lai,G.-M., Zinn,S. and Allegra,C.J. (1990) Resistance of a human ovarian cancer line to 5-fluorouracil associated with decreased levels of 5-fluorouracil in DNA. Mol. Pharmacol., 38, 410-417.
20. Dunlap,R.B., Harding,N.G.L. and Huennekens,F.M. (1971) Thymidylate synthase and its relationship to dihydrofolate reductase. Ann. N. Y. Acad. Sci., 186, 153-165.
21. Bernardi,A. and Spahr,P.F. (1972) Nucleotide sequence at the binding site for coat protein on RNA of bacteriophage R17. Proc. Natl Acad. Sci. USA, 69, 3033-3037.
22. Carey,J. and Uhlenbeck,O.C. (1983) Sequence-specific interaction of R17 coat protein-ribonucleic acid interaction. Biochemistry, 22, 2601-2615.
23. Romaniuk,P.J. and Uhlenbeck,O.C. (1985) Nucleoside and nucleotide inactivation of the R-17 coat protein: evidence for the transient covalent RNA-protein bond. Biochemistry, 24, 4239-4244.
24. Koontz,S.W. and Schimmel,P.R. (1979) Aminoacyl-tRNA synthetase-catalyzed cleavage of the glycosidic bond of 5-halogenated uridines. J. Biol. Chem., 254, 12277-12284.
25. Starzyk,R.M., Koontz,S.W. and Schimmel,P.R. (1982) A covalent adduct between the uracil ring and the active site of an aminoacyl tRNA synthetase. Nature, 298, 136-140.
26. Klausner,R.D., Rouault,T.A. and Harford,J.B. (1993) Regulating the fate of mRNA: the control of cellular iron metabolism. Cell, 72, 19-28.
27. Hentze,M.W., Rouault,T.A., Harford,J.B. and Klausner,R.D. (1989) Oxidation-reduction and the molecular mechanism of a regulatory RNA-protein interaction. Science, 244, 357-359.
28. Matthews,D.A., Appelt,K., Oatley,S.J. and Xuong,N.H. (1990) Crystal structure of Escherichia coli thymidylate synthase containing bound 5-fluoro-2′-deoxyuridylate and 10-propargyl-5, 8,-dideazafolate. J. Mol. Biol., 214, 937-948.
29. Montfort,W.R., Perry,K.M., Fauman,E.B., Finer-Moore,J.S., Maley,G.F., Hardy,L., Maley,F. and Stroud,R.M. (1990) Structure, multiple site binding and segmental accommodation in thymidylate synthase on binding dUMP and an anti-folate. Biochemistry, 29, 6964-6977.
30. Hyatt,D.C., Maley,F. and Montfort,W.R. (1977) Use of strain in a stereospecific catalytic mechanism: crystal structures of Escherichia coli thymidylate synthase bound to FdUMP and methylenetetrahydrofolate. Biochemistry, 36, 4584-4594.
31. Phan,J., Koli,S., Minor,W., Dunlap,R.B., Berger,S.H. and Lebioda,L.(2001) Human thymidylate synthase is in the closed conformation when complexed with dUMP and raltitrexed, an antifolate drug. Biochemistry, 40, 1897-1902.
32. Almog,R., Waddling,C.A., Maley,F., Maley,G.F. and Van Roey,P. (2001) Crystal structure of a deletion mutant of human thymidylate synthase delta(7-29) and its ternary complex with tomudex and dUMP. Protein Sci., 10, 988-996.