메뉴 건너뛰기




Volumn 134, Issue 4, 2003, Pages 505-511

Characterization of ATPase Activity of a Hepatitis C Virus NS3 Helicase Domain, and Analysis Involving Mercuric Reagents

Author keywords

ATPase activity; DEAD box protein; Expression; HCV; Mercuric reagent; NS3 helicase domain; Purification

Indexed keywords

ADENOSINE TRIPHOSPHATASE; CYSTEINE; DITHIOTHREITOL; HELICASE; MAGNESIUM ION; MERCURIC CHLORIDE; RNA;

EID: 0344873225     PISSN: 0021924X     EISSN: None     Source Type: Journal    
DOI: 10.1093/jb/mvg167     Document Type: Article
Times cited : (9)

References (41)
  • 1
    • 0024509701 scopus 로고
    • Isolation of a cDNA clone derived from a blood-borne non-A, non-B viral hepatitis genome
    • Choo, Q.L., Kuo, G., Weiner, A.J., Overby, L.R., Bradley, D.W., and Houghton, M. (1989) Isolation of a cDNA clone derived from a blood-borne non-A, non-B viral hepatitis genome. Science 244, 359-362
    • (1989) Science , vol.244 , pp. 359-362
    • Choo, Q.L.1    Kuo, G.2    Weiner, A.J.3    Overby, L.R.4    Bradley, D.W.5    Houghton, M.6
  • 4
    • 0027450808 scopus 로고
    • Hepatitis C virus RNA in southern African blacks with hepatocellular carcinoma
    • Bukh, J., Miller, R.H., Kew, M.C., and Purcell, R.H. (1993) Hepatitis C virus RNA in southern African blacks with hepatocellular carcinoma. Proc. Natl Acad. Sci. USA 90, 1848-1851
    • (1993) Proc. Natl Acad. Sci. USA , vol.90 , pp. 1848-1851
    • Bukh, J.1    Miller, R.H.2    Kew, M.C.3    Purcell, R.H.4
  • 6
    • 0025249362 scopus 로고
    • Hepatitis C virus shares amino acid sequence similarity with pestiviruses and flaviviruses as well as members of two plant virus supergroups
    • Miller, R.H., and Purcell, R.H. (1990) Hepatitis C virus shares amino acid sequence similarity with pestiviruses and flaviviruses as well as members of two plant virus supergroups. Proc. Natl Acad. Sci. USA 87, 2057-2061
    • (1990) Proc. Natl Acad. Sci. USA , vol.87 , pp. 2057-2061
    • Miller, R.H.1    Purcell, R.H.2
  • 9
    • 0025991559 scopus 로고
    • Gene mapping of the putative structural region of the hepatitis C virus genome by in vitro processing analysis
    • Hijikata, M., Kato, N., Ootsuyama, Y., Nakagawa, M., and Shimotohno, K. (1991) Gene mapping of the putative structural region of the hepatitis C virus genome by in vitro processing analysis. Proc. Natl Acad. Sci. USA 88, 5547-5551
    • (1991) Proc. Natl Acad. Sci. USA , vol.88 , pp. 5547-5551
    • Hijikata, M.1    Kato, N.2    Ootsuyama, Y.3    Nakagawa, M.4    Shimotohno, K.5
  • 11
    • 0027163740 scopus 로고
    • Two distinct proteinase activities required for the processing of a putative nonstructural precursor protein of hepatitis C virus
    • Hijikata, M., Mizushima, H., Akagi, T., Mori, S., Kakiuchi, N., Kato, N., Tanaka, T., Kimura, K., and Shimotohno, K. (1993) Two distinct proteinase activities required for the processing of a putative nonstructural precursor protein of hepatitis C virus. J. Virol. 67, 4665-4675
    • (1993) J. Virol. , vol.67 , pp. 4665-4675
    • Hijikata, M.1    Mizushima, H.2    Akagi, T.3    Mori, S.4    Kakiuchi, N.5    Kato, N.6    Tanaka, T.7    Kimura, K.8    Shimotohno, K.9
  • 12
    • 0027476809 scopus 로고
    • Expression and identification of hepatitis C virus polyprotein cleavage products
    • Grakoui, A., Wychowski, C., Lin, C., Feinstone, S.M., and Rice, C.M. (1993) Expression and identification of hepatitis C virus polyprotein cleavage products. J. Virol. 67, 1385-1395
    • (1993) J. Virol. , vol.67 , pp. 1385-1395
    • Grakoui, A.1    Wychowski, C.2    Lin, C.3    Feinstone, S.M.4    Rice, C.M.5
  • 14
    • 0027414062 scopus 로고
    • Characterization of the hepatitis C virus-encoded serine proteinase: Determination of proteinase-dependent polyprotein cleavage sites
    • Grakoui, A., McCourt, D.W., Wychowski, C., Feinstone, S.M., and Rice, C.M. (1993) Characterization of the hepatitis C virus-encoded serine proteinase: determination of proteinase-dependent polyprotein cleavage sites. J. Virol. 67, 2832-2843
    • (1993) J. Virol. , vol.67 , pp. 2832-2843
    • Grakoui, A.1    McCourt, D.W.2    Wychowski, C.3    Feinstone, S.M.4    Rice, C.M.5
  • 15
    • 0027287798 scopus 로고
    • Nonstructural protein 3 of the hepatitis C virus encodes a serine-type proteinase required for cleavage at the NS3/4 and NS4/5 junctions
    • Bartenschlager, R., Ahlborn-Laake, L., Mous, J., and Jacobsen, H. (1993) Nonstructural protein 3 of the hepatitis C virus encodes a serine-type proteinase required for cleavage at the NS3/4 and NS4/5 junctions. J. Virol. 67, 3835-3844
    • (1993) J. Virol. , vol.67 , pp. 3835-3844
    • Bartenschlager, R.1    Ahlborn-Laake, L.2    Mous, J.3    Jacobsen, H.4
  • 16
    • 0027176287 scopus 로고
    • NS3 is a serine protease required for processing of hepatitis C virus polyprotein
    • Tomei, L., Failla, C., Santolini, E., De Francesco, R., and La Monica, N. (1993) NS3 is a serine protease required for processing of hepatitis C virus polyprotein. J. Virol. 67, 4017-4026
    • (1993) J. Virol. , vol.67 , pp. 4017-4026
    • Tomei, L.1    Failla, C.2    Santolini, E.3    De Francesco, R.4    La Monica, N.5
  • 17
    • 0027170423 scopus 로고
    • The hepatitis C virus encodes a serine protease involved in processing of the putative nonstructural proteins from the viral polyprotein precursor
    • Eckart, M.R., Selby, M., Masiarz, F., Lee, C., Berger, K., Crawford, K., Kuo, C., Kuo, G., Houghton, M., and Choo, Q.L. (1993) The hepatitis C virus encodes a serine protease involved in processing of the putative nonstructural proteins from the viral polyprotein precursor. Biochem. Biophys. Res. Commun. 192, 399-406
    • (1993) Biochem. Biophys. Res. Commun. , vol.192 , pp. 399-406
    • Eckart, M.R.1    Selby, M.2    Masiarz, F.3    Lee, C.4    Berger, K.5    Crawford, K.6    Kuo, C.7    Kuo, G.8    Houghton, M.9    Choo, Q.L.10
  • 18
    • 0027931010 scopus 로고
    • Processing of hepatitis C viral polyprotein in Escherichia coli
    • Tanji, Y., Hijikata, M., Hirowatari, Y., and Shimotohno, K. (1994) Processing of hepatitis C viral polyprotein in Escherichia coli. Gene 145, 215-219
    • (1994) Gene , vol.145 , pp. 215-219
    • Tanji, Y.1    Hijikata, M.2    Hirowatari, Y.3    Shimotohno, K.4
  • 19
    • 0030592514 scopus 로고    scopus 로고
    • The crystal structure of hepatitis C virus NS3 proteinase reveals a trypsin-like fold and a structural zinc binding site
    • Love, R.A. Parge, H.E., Wickersham, J.A., Hostomsky, Z., Habuka, N., Moomaw, E.W., Adachi, T., and Hostomska, Z. (1996) The crystal structure of hepatitis C virus NS3 proteinase reveals a trypsin-like fold and a structural zinc binding site. Cell 87, 331-342
    • (1996) Cell , vol.87 , pp. 331-342
    • Love, R.A.1    Parge, H.E.2    Wickersham, J.A.3    Hostomsky, Z.4    Habuka, N.5    Moomaw, E.W.6    Adachi, T.7    Hostomska, Z.8
  • 21
    • 0027199917 scopus 로고
    • Hepatitis C virus NS3 protein polynucleotide-stimulated nucleoside triphosphatase and comparison with the related pestivirus and flavivirus enzymes
    • Suzich, J.A., Tamura, J.K., Palmer-Hill, F., Warrener, P., Grakoui, A., Rice, C.M., Feinstone, S.M., and Collett, M.S. (1993) Hepatitis C virus NS3 protein polynucleotide-stimulated nucleoside triphosphatase and comparison with the related pestivirus and flavivirus enzymes. J. Virol. 67, 6152-6158
    • (1993) J. Virol. , vol.67 , pp. 6152-6158
    • Suzich, J.A.1    Tamura, J.K.2    Palmer-Hill, F.3    Warrener, P.4    Grakoui, A.5    Rice, C.M.6    Feinstone, S.M.7    Collett, M.S.8
  • 22
    • 0028817781 scopus 로고
    • C-terminal domain of the hepatitis C virus NS3 protein contains an RNA helicase activity
    • Kim, D.W., Gwack, Y., Han, J.H., and Choe, J. (1995) C-terminal domain of the hepatitis C virus NS3 protein contains an RNA helicase activity. Biochem. Biophys. Res. Commun. 215, 160-166
    • (1995) Biochem. Biophys. Res. Commun. , vol.215 , pp. 160-166
    • Kim, D.W.1    Gwack, Y.2    Han, J.H.3    Choe, J.4
  • 23
    • 0028884176 scopus 로고
    • Expression, isolation, and characterization of the hepatitis C virus ATPase/RNA helicase
    • Jin, L. and Peterson, D.L. (1995) Expression, isolation, and characterization of the hepatitis C virus ATPase/RNA helicase. Arch. Biochem. Biophys. 323, 47-53
    • (1995) Arch. Biochem. Biophys. , vol.323 , pp. 47-53
    • Jin, L.1    Peterson, D.L.2
  • 24
    • 0029950090 scopus 로고    scopus 로고
    • Enzymatic characterization of hepatitis C virus NS3/4A complexes expressed in mammalian cells by using the herpes simplex virus amplicon system
    • Hong, Z., Ferrari, E., Wright-Minogue, J., Chase, R., Risano, C., Seelig, G., Lee, C.-G., and Kwong, A.-D. (1996) Enzymatic characterization of hepatitis C virus NS3/4A complexes expressed in mammalian cells by using the herpes simplex virus amplicon system. J. Virol. 70, 4261-4268
    • (1996) J. Virol. , vol.70 , pp. 4261-4268
    • Hong, Z.1    Ferrari, E.2    Wright-Minogue, J.3    Chase, R.4    Risano, C.5    Seelig, G.6    Lee, C.-G.7    Kwong, A.-D.8
  • 25
    • 0030583249 scopus 로고    scopus 로고
    • Characterization of RNA binding activity and RNA helicase activity of the hepatitis C virus NS3 protein
    • Gwack, Y., Kim, D.W., Han, J.H., and Choe, J. (1996) Characterization of RNA binding activity and RNA helicase activity of the hepatitis C virus NS3 protein. Biochem. Biophys. Res. Commun. 225, 654-659
    • (1996) Biochem. Biophys. Res. Commun. , vol.225 , pp. 654-659
    • Gwack, Y.1    Kim, D.W.2    Han, J.H.3    Choe, J.4
  • 26
    • 0029784485 scopus 로고    scopus 로고
    • A steady-state and pre-steady-state kinetic analysis of the NTPase activity associated with the hepatitis C virus NS3 helicase domain
    • Preugschat, F., Averett, D.R., Clarke, B.E., and Porter, D.J.T. (1996) A steady-state and pre-steady-state kinetic analysis of the NTPase activity associated with the hepatitis C virus NS3 helicase domain. J. Biol. Chem. 271, 24449-24457
    • (1996) J. Biol. Chem. , vol.271 , pp. 24449-24457
    • Preugschat, F.1    Averett, D.R.2    Clarke, B.E.3    Porter, D.J.T.4
  • 27
    • 0029970903 scopus 로고    scopus 로고
    • The helicase activity associated with hepatitis C virus non-structural protein 3 (NS3)
    • Tai, C.-L., Chi, W.-K., Chen, D.-S., and Hwang, L.-H. (1996) The helicase activity associated with hepatitis C virus non-structural protein 3 (NS3). J. Virol. 70, 8477-8484
    • (1996) J. Virol. , vol.70 , pp. 8477-8484
    • Tai, C.-L.1    Chi, W.-K.2    Chen, D.-S.3    Hwang, L.-H.4
  • 28
    • 0033920304 scopus 로고    scopus 로고
    • Hepatitis C virus-encoded enzymatic in the 3′ nontranslated region are essential for virus replication in vivo
    • Kolyhalov, A.A., Mihalik, K., Feinstone, S.M., and Rice, C.M. (2000) Hepatitis C virus-encoded enzymatic in the 3′ nontranslated region are essential for virus replication in vivo. J. Virol. 74, 2046-2051
    • (2000) J. Virol. , vol.74 , pp. 2046-2051
    • Kolyhalov, A.A.1    Mihalik, K.2    Feinstone, S.M.3    Rice, C.M.4
  • 29
    • 0024425153 scopus 로고
    • Homologous potyvirus and flavivirus proteins belonging to a superfamily of helicase-like proteins
    • Lain, S.J., Riechman, L., Martin, M.T., and Garcia, J.A. (1989) Homologous potyvirus and flavivirus proteins belonging to a superfamily of helicase-like proteins. Gene 82, 357-362
    • (1989) Gene , vol.82 , pp. 357-362
    • Lain, S.J.1    Riechman, L.2    Martin, M.T.3    Garcia, J.A.4
  • 30
    • 0027254336 scopus 로고
    • RNA-stimulated NTPase activity associated with the p80 protein of the pestivirus bovine viral diarrhea virus
    • Tamura, J.K., Warrener, P., and Collett, M.S. (1993) RNA-stimulated NTPase activity associated with the p80 protein of the pestivirus bovine viral diarrhea virus. Virology 193, 1-10
    • (1993) Virology , vol.193 , pp. 1-10
    • Tamura, J.K.1    Warrener, P.2    Collett, M.S.3
  • 31
    • 0027446550 scopus 로고
    • RNA-stimulated NTPase activity associated with yellow fever virus NS3 protein expressed in bacteria
    • Warrener, P., Tamura, J.K., and Collett, M.S. (1993) RNA-stimulated NTPase activity associated with yellow fever virus NS3 protein expressed in bacteria. J. Virol. 67, 989-996
    • (1993) J. Virol. , vol.67 , pp. 989-996
    • Warrener, P.1    Tamura, J.K.2    Collett, M.S.3
  • 32
    • 0032520794 scopus 로고    scopus 로고
    • Detection of hepatitis C virus helicase activity using the scintillation proximity assay system
    • Kyono, K., Miyashiro, M., and Taguchi, I. (1998) Detection of hepatitis C virus helicase activity using the scintillation proximity assay system. Anal. Biochem. 257, 120-126
    • (1998) Anal. Biochem. , vol.257 , pp. 120-126
    • Kyono, K.1    Miyashiro, M.2    Taguchi, I.3
  • 35
    • 0028800155 scopus 로고
    • Poly(U) binding activity of hepatitis C virus NS3 protein, a putative RNA helicase
    • Kanai, A., Tanabe, K., and Kohara, M. (1995) Poly(U) binding activity of hepatitis C virus NS3 protein, a putative RNA helicase. FEBS Lett. 376, 221-224
    • (1995) FEBS Lett. , vol.376 , pp. 221-224
    • Kanai, A.1    Tanabe, K.2    Kohara, M.3
  • 36
    • 0028872259 scopus 로고
    • Pestivirus NS3 (p80) protein possesses RNA helicase activity
    • Warrener, P. and Collett, M.S. (1995) Pestivirus NS3 (p80) protein possesses RNA helicase activity. J. Virol. 69, 1720-1726
    • (1995) J. Virol. , vol.69 , pp. 1720-1726
    • Warrener, P.1    Collett, M.S.2
  • 37
    • 0030897821 scopus 로고    scopus 로고
    • Virus-encoded RNA helicases
    • Kadare, G. and Haenni, A.L. (1997) Virus-encoded RNA helicases. J. Virol. 71, 2583-2590
    • (1997) J. Virol. , vol.71 , pp. 2583-2590
    • Kadare, G.1    Haenni, A.L.2
  • 38
    • 0027182114 scopus 로고
    • Helicases: Amino acid sequence comparisons and structure-function relationships
    • Gorbalenya, A.E. and Koonin, E.V. (1993) Helicases: amino acid sequence comparisons and structure-function relationships. Curr. Opin. Struct. Biol. 3, 419-429
    • (1993) Curr. Opin. Struct. Biol. , vol.3 , pp. 419-429
    • Gorbalenya, A.E.1    Koonin, E.V.2
  • 39
    • 0001607723 scopus 로고
    • Distantly related sequences in the alpha- and beta-subunits of ATP synthase, myosin, kinases and other ATP-requiring enzymes and a common nucleotide binding fold
    • Walker, J.E., Saraste, M., Runswick, M.J., and Gay, N.J. (1982) Distantly related sequences in the alpha- and beta-subunits of ATP synthase, myosin, kinases and other ATP-requiring enzymes and a common nucleotide binding fold. EMBO J. 1, 945-951
    • (1982) EMBO J. , vol.1 , pp. 945-951
    • Walker, J.E.1    Saraste, M.2    Runswick, M.J.3    Gay, N.J.4
  • 40
    • 0013627747 scopus 로고    scopus 로고
    • Mutational analysis of the hepatitis C virus RNA helicase
    • Kim, D.W., Kim, J., Gwack, Y., Han, J.H., and Choe, J. (1997) Mutational analysis of the hepatitis C virus RNA helicase. J. Virol. 71, 9400-9409
    • (1997) J. Virol. , vol.71 , pp. 9400-9409
    • Kim, D.W.1    Kim, J.2    Gwack, Y.3    Han, J.H.4    Choe, J.5
  • 41
    • 0034870450 scopus 로고    scopus 로고
    • Structure-based mutational analysis of the hepatitis C virus NS3 helicase
    • Tai, C.-L., Pan, W.-C., Liaw, S.-H., Yang, U.-C., Hwang, L.-H., and Chen, D.-S. (2001) Structure-based mutational analysis of the hepatitis C virus NS3 helicase. J. Virol. 75, 8289-8297
    • (2001) J. Virol. , vol.75 , pp. 8289-8297
    • Tai, C.-L.1    Pan, W.-C.2    Liaw, S.-H.3    Yang, U.-C.4    Hwang, L.-H.5    Chen, D.-S.6


* 이 정보는 Elsevier사의 SCOPUS DB에서 KISTI가 분석하여 추출한 것입니다.