Gc Protein-derived Macrophage Activating Factor (GcMAF): Isoelectric Focusing Pattern and Tumoricidal Activity

Anticancer Research

Volumn 23, Issue 6 A, 2003, Pages 4451-4457

  Mohamad, Saharuddin Bin ^a   Nagasawa, Hideko ^a   Sasaki, Hideyuki ^a   Uto, Yoshihiro ^a   Nakagawa, Yoshinori ^b   Kawashima, Ken ^c   Hori, Hitoshi ^a  

^a UNIVERSITY OF TOKUSHIMA   (Japan)

^b HAYASHIBARA BIOCHEMICAL LABORATORIES INC   (Japan)

^c Meneki Bunseki Kenkyu Ctr Co (MBK)   (Japan)

Author keywords

Gc protein derived macrophage activating factor (GcMAF); Isoelectric focusing (IEF); Nitric oxide (NO); Tumor necrosis factor (TNF); Tumoricidal

Indexed keywords

GLYCOPROTEIN; GROUP SPECIFIC COMPONENT GLOBULIN; MACROPHAGE ACTIVATING FACTOR; NITRIC OXIDE; UNCLASSIFIED DRUG;

ANALYTIC METHOD; ANIMAL CELL; BIOASSAY; CARBOHYDRATE ANALYSIS; COCULTURE; CONFERENCE PAPER; CONTROLLED STUDY; ELECTROPHORETIC MOBILITY; EVALUATION; FEMALE; INTERMETHOD COMPARISON; ISOELECTRIC FOCUSING; METASTASIS INHIBITION; MOUSE; NONHUMAN; PRIORITY JOURNAL;

ADULT; AGED; AGED, 80 AND OVER; ANIMALS; CELL LINE, TUMOR; COCULTURE TECHNIQUES; FEMALE; HUMANS; IMMUNOTHERAPY, ADOPTIVE; ISOELECTRIC FOCUSING; MACROPHAGE-ACTIVATING FACTORS; MACROPHAGES; MICE; MICE, INBRED ICR; MIDDLE AGED; NEOPLASMS, EXPERIMENTAL; NITRIC OXIDE; TUMOR NECROSIS FACTOR-ALPHA; VITAMIN D-BINDING PROTEIN;

EID: 0344872745     PISSN: 02507005     EISSN: None     Source Type: Journal    
DOI: None     Document Type: Conference Paper

References (21)

1. Tedla N, Palladinetti P, Wakefield D and Lloyd A: Abundant expression of chemokines in malignant and infective human lymphadenopathies. Cytokine 11: 531-540, 1999.
2. Seljelid R and Busund LT: The biology of macrophages: II. Inflammation and tumors. Eur J Haematol 52: 1-12, 1994.
3. Yamamoto N and Homma S: Vitamin D3 binding protein (group-specific component) is a precursor for the macrophage-activating signal factor from lysophosphotidylcholine-treated lymphocytes. Proc Natl Acad Sci USA 88: 8539-8543, 1991.
4. Haddad JG and Walgate J: 25-Hydroxyvitamin D transport in human plasma: isolation and partial characterization of calciferol-binding protein. J Biol Chem 251: 4803-4809, 1976.
5. Yamamoto N and Kumashiro R: Conversion of vitamin D3 binding protein (group-specific component) to a macrophage activating factor by the stepwise action of beta-galactosidase of B cells and sialidase of T cells. J Immunol 151: 2794-2802, 1993.
6. Yamamoto N: Structural definition of a potent macrophage activating factor derived from vitamin D3-binding protein with adjuvant activity for antibody production. Mol Immunol 33: 1157-1164, 1996.
7. Svasti J, Kurosky A, Bennett A and Bowman H: Molecular basis for the three major forms of human serum vitamin D binding protein (group-specific component). Biochemistry 18: 1611-1617, 1979.
8. Svasti J and Bowman B H: Human group-specific component. Changes in electrophoretic mobility resulting from vitamin D binding and from neuraminidase digestion. J Biol Chem 253: 4188-4194, 1978.
9. Takaki T and Ujiie K: Gc typing using PhastGell® Dry IEF containing separators by PhastSystem™ Res Pract Forens Med 36: 59-62, 1993.
10. Kubo S, Kitamura O, Tsuda R, Hirose W, Matsumoto H and Nakano I: Hereditary recombined three-allele variant of the Gc system. Hum Genet 91: 71-71 1993.
11. Mohamad SB, Uto Y, Nagasawa H and Hori H: Preparation of Gc protein-derived macrophage activating factor (GcMAF) and its structural characterization and biological activities. Anticancer Res 22: 4297-4300, 2002.
12. Flick DA and Gifford GE: Comparison of in vitro cell cytotoxicity assays for tumor necrosis factor. J Immunol Methods 68: 167-175, 1984.
13. Stuehr DJ and Marletta MA: Synthesis of nitrate in murine macrophage cell lines. Cancer Res 47: 5590-5594, 1987.
14. Yamamoto N: Macrophage activation factor from vitamin D binding protein. U.S. Patent 5,326,749, 1994.
15. Mohamad SB, Hori H, Nagasawa H, Usui K and Uto Y: Characterization of human Gc protein-derived macrophage activation factor (GcMAF) and its functional role in macrophage tumoricidal activity. Adv Exp Med Biol 510: 77-82, 2003.
16. Aliprantis AO, Diez-Roux G, Mulder LC, Zychlinsky A and Lang RA: Do macrophages kill through apoptosis? Immunol Today 17: 573-576, 1996.
17. Mytar B, Siedlar M, Woloszyn M, Ruggiero I, Pryjma J and Zembala M: Induction of reactive oxygen intermediates in human monocytes by tumor cell and their role in spontaneous monocytes cytotoxicity. Br J Cancer 79: 737-743, 1999.
18. Ono T, Ichikawa H, Asami R and Yamamoto I: Enzymatically modified Gc gobulin induces the translocation of FcγRI and FcγRII from intracellular storage compartments to the cell surface in murine peritoneal macrophage. Jpn J Inflammation 15: 293-299, 1995.
19. Swamy N, Ghosh S, Schneider GB and Ray R: Baculovirus-expressed vitamin D-binding protein-macrophage activating factor (DBP-maf) activates osteoclasts and binding of 25-hydroxyvitamin D(3) does not influence this activity. J Cell Biochem 81: 535-546, 2001.
20. Mohamad SB, Nagasawa H, Uto Y and Hori H: Tumor cell alpha-N-acetylgalactosaminidase activity and its involvement in GcMAF-related macrophage activation. Comp Biochem Physiol A Mol Integr Physiol 132: 1-8, 2002.
21. Kanda S, Mochizuki Y, Miyata Y, Kanetake H and Yamamoto N: Effects of vitamin D(3)-binding protein-derived macrophage activating factor (GcMAF) on angiogenesis. J Natl Cancer Inst 94: 1311-1319, 2002.