Immunohistochemical and in situ mRNA hybridisation techniques to determine the distribution of ion channels in human brain: A study of neuronal voltage-dependent calcium channels

Brain Research Protocols

Volumn 1, Issue 3, 1997, Pages 299-306

  McCormack, Alison L ^a   Day, Nicola C ^b   Craig, Peter J ^a   Smith, William ^a   Beattie, Ruth E ^a   Volsen, Stephen G ^a  

^a ELI LILLY AND COMPANY   (United States)

^b NEWCASTLE GENERAL HOSPITAL   (United Kingdom)

Author keywords

Calcium channel; Human brain; Immunohistochemistry; MRNA hybridisation, in situ

Indexed keywords

CALCIUM CHANNEL; MESSENGER RNA;

ARTICLE; BRAIN; CONTROLLED STUDY; HUMAN; HUMAN TISSUE; IMMUNOHISTOCHEMISTRY; IN SITU HYBRIDIZATION; NEUROTRANSMITTER RELEASE; PRIORITY JOURNAL; SYNAPTIC TRANSMISSION;

AUTORADIOGRAPHY; BRAIN; CALCIUM CHANNELS; ELECTROPHYSIOLOGY; HUMANS; IMMUNOHISTOCHEMISTRY; IN SITU HYBRIDIZATION; NEURONS; RNA, MESSENGER; TISSUE DISTRIBUTION;

EID: 0344863133     PISSN: 1385299X     EISSN: None     Source Type: Journal    
DOI: 10.1016/S1385-299X(97)00003-2     Document Type: Article

References (20)

1. Antoon, F.M., Moorman, P., De Boer, A.J., Vermeulen, J.L. and Lamers, W.H., Practical aspects of radio-isotopic in situ hybridisation of RNA, Histochem. J., 25 (1993) 251-266.
2. Barton, A.J., Pearson, R.C., Najlerahim, A. and Harrison, P.J., Pre- and postmortem influences on brain RNA, J. Neurochem., 61 (1993) 1-11.
3. Bowersox, S.S., Valentino, K.L. and Luther, R.R., Neuronal voltage sensitive calcium channels, Drug News Perspect., 7 (1994) 261-268.
4. Catterall, W.A., Structure and function of voltage gated ion channels, Trends Neurosci., 16 (1993) 500-506.
5. DeMey, J., Colloidal gold probes in immunohistochemistry. In: J.M. Polak and S.V. Noorden (Eds.), Immunocytochemistry. Practical Applications in Pathology and Biology, Wright and Sons, Bristol, 1983, pp. 82-112.
6. Fujita, Y. et al., Primary structure and functional expression of the ω-conotoxin sensitive N type calcium channel from rabbit brain, Neuron, 10 (1993) 585-598.
7. Mintz, I.M., Adams, M.E. and Bean, B.P., P-type calcium channels in rat central and peripheral neurones, Neuron, 9 (1992) 85-95.
8. Olivera, B.M., Miljanich, G.P., Ramachandran, J. and Adams, M.E., Calcium channel diversity and neurotransmitter release: the ω-conotoxins and ω-agatoxins, Annu. Rev. Biochem., 63 (1994) 823-867.
9. Pringle, J.H. et al., In situ hybridisation of immunoglobulin light chain mRNA in paraffin sections using biotinylated or hapten labelled oligonucleotide probes, J. Pathol., 162 (1990) 197-207.
10. Schafer, M.K., Stoffers, D.A., Eipper, B.A. and Watson, S.J., Expression of peptidoglycan alpha-amidating monooxygenase (EC 1.14.17.3) in the rat central nervous system, J. Neurosci., 12 (1992) 222-234.
11. Scott, R.H., Pearson, H.A. and Dolphin, A.C., Aspects of vertebrate neuronal voltage-activated calcium currents and their regulation, Prog. Neurobiol., 36 (1991) 485-520.
12. Snutch, T.P., Tomlinson, W.J., Leonard, J.P. and Gilbert, M.M., Distinct calcium channels are generated by alternative splicing and are differentially expressed in the mammalian CNS, Neuron, 7 (1991) 45-57.
13. 1- and β-subunits in developing and mature rat brain, Mol. Brain Res., 30 (1995) 1-16.
14. 2+ channels, Trends Pharmacol. Sci., 12 (1991) 349-354.
15. Volsen, S.G. et al., The expression of neuronal voltage-dependent calcium channels in human cerebellum, Mol. Brain Res., 34 (1995) 271-282.
16. 1 subunit, Neuron, 9 (1992) 1099-1115.
17. Wilcox, J.N., Fundamental principles of in situ hybridisation, J. Histochem. Cytochem., 41 (1993) 1725-1733.
18. Williams, M.E. et al., Structure and functional expression of an ω-conotoxin-sensitive human N-type calcium channel, Science, 257 (1992) 389-395.
19. 1E calcium channel subtypes, J. Biol. Chem., 269 (1994) 22347-22357.
20. 1A high voltage-activated calcium channels, Soc. Neurosci. Abstr., 20 (1994) 68.