The extractive purification of peroxidase from plant raw materials in aqueous two-phase systems

Acta Biotechnologica

Volumn 18, Issue 3, 1998, Pages 179-188

  Miranda, M V ^a   Fernandez Lahore H M ^a   Dobrecky, J ^a   Cascone, O ^a,b  

^a UNIVERSIDAD DE BUENOS AIRES   (Argentina)

^b Cátedra de Farmacotecnia II ^*  (Argentina)

Author keywords

[No Author keywords available]

Indexed keywords

ACETIC ACID; CONCANAVALIN A; MICROSPHERE; PEROXIDASE; TRITON X 100;

AFFINITY CHROMATOGRAPHY; ARTICLE; ENZYME PURIFICATION; EXTRACTION; PH; PLANT ROOT; PLANT SEED; TEMPERATURE;

ARMORACIA RUSTICANA; GLYCINE MAX;

EID: 0344863120     PISSN: 01384988     EISSN: None     Source Type: Journal    
DOI: 10.1002/abio.370180302     Document Type: Article

References (22)

1. DUNFORD, H. B., STILLMAN, J. S.: On the function and mechanism of action of peroxidases. Coord. Chem. Rev. 19 (1976), 187-251.
2. SHANNON, L. M., KAY, E., LEW, J. Y.: Peroxidase isozymes from horseradish roots. J. Biol. Chem. 241 (1966), 2166-2172.
3. GILLIKIN, J. W., GRAHAM, J. S.: Purification and developmental analysis of the major anionic peroxidase from the seed coat of Glycine max. Plant Physiol. 96 (1991), 214-220.
4. TJISSEN, P.: Enzymes for immunoassays. In: Practice and Theory of Enzyme Immunoassays. (BURDON, R. H., VAN KNIPPENBERG, P. H., eds.), Amsterdam, New York, Oxford: Elsevier, 1985, Chapt. 10, 173-220.
5. KLIBANOV, A. M., TSU-MAN, T., SCOTT, K. P.: Peroxidase catalyzed removal of phenols from coal-conversion wastewaters. Science 221 (1983), 259-261.
6. NICELL, J. A., BEWTRA, J. K., BISWAS, N., ST. PIERRE, C. C., TAYLOR, K. E.: Enzyme catalyzed polymerization and precipitation of aromatic compounds from aqueous solution. Can. J. Civ. Eng. 20 (1993), 725-735.
7. NICELL, J. A., SAADI, K. W., BUCHANAN, I. D.: Phenol polymerization and precipitation by horseradish peroxidase enzyme and an additive. Bioresource Technol. 54 (1995), 5-16.
8. MAC ELDOON, J. P., DORDICK, J. S.: Unusual thermal stability of soybean peroxidase. Biotechnol. Prog. 12 (1996), 555-558.
9. ABBOTT, N. L., BLANKSCHTEIN, D., HATTON, T. A.: On protein partitioning in two-phase aqueous polymer systems. Bioseparation 1 (1990), 191-225.
10. ALBERTSSON, P. A. In: Partition of Cell Particles and Macromolecules. John Wiley Interscience (third edition), New York, 1986.
11. KULA, M. R.: Trends and future of aqueous two-phase extraction. Bioseparation 1 (1990), 181-189.
12. VILTER, H.: Aqueous two-phase extraction of plant enzymes from sources containing large amounts of tannins and anionic mucilages. Bioseparation 1 (1990), 283-292.
13. SANCHEZ-FERRER, A., BRU, R., GARCIA CARMONA, F.: Novel procedure for extraction of a latent grape polyphenoloxidase using temperature-induced phase separation in Triton X-114. Plant Physiol. 91 (1989), 1481-1487.
14. SANCHEZ-FERRER, A., BRU, R., GARCIA CARMONA, F.: Partial purification of a thylakoid-bound enzyme using temperature-induced phase partitioning. Analyt. Biochem. 184 (1990), 279-282.
15. KONDO, A., KANEKO, T., HIGASHITANI, K.: Purification of fusion proteins using affinity microspheres in aqueous two-phase systems. Appl. Microbiol. Biotechnol. 40 (1993), 365-369.
16. TERSTAPPEN, G. C., RAMELMEIER, R. A., KULA, M. R.: Protein partitioning in detergent-based aqueous two-phase systems. J. Biotechnol. 28 (1993), 263-275.
17. MIRANDA, M. V., CASCONE, O.: Partition behaviour of horseradish peroxidase isoenzymes in aqueous two-phase poly(ethyleneglycol)/phosphate systems. Biotechnol. Tech. 8 (1994), 275-280.
18. BRADFORD, M.: A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding. Analyt. Biochem. 72 (1976), 248-254.
19. SMITH, P. K., KROHN, R. I., HERMANSON, G. T., MALLIA, A. K., GARTNER, F. H., PROVENZANO, M. D., FIJIMOTO, E. K., GOEKE, N. M., OLSON, B. J., KLENK, D. C.: Measurement of protein using bicinchinonic acid. Analyt. Biochem. 150 (1985), 76-85.
20. PRICE, M. L., BUTLER, L. G.: Rapid visual estimation and spectrophotometric determination of tannin content of sorghum grain. J. Agric. Food Chem. 25 (1977), 1268-1273.
21. LAEMMLI, U. K.: Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature 227 (1970), 680-685.
22. MIRANDA, M. V., FERNANDEZ LAHORE, H. M., CASCONE, O.: Horseradish peroxidase extraction and purification by aqueous two-phase partition. Appl. Biochem. Biotechnol. 53 (1995), 147-154.