Fast, convenient, and effective method to transiently transfect primary hippocampal neurons

Journal of Neuroscience Research

Volumn 58, Issue 6, 1999, Pages 831-835

  Köhrmann, Martin ^a,b   Haubensak, Wulf ^c   Hemraj, Indradeo ^a,b   Kaether, Christoph ^a   Leßmann, Volkmar J ^c   Kiebler, Michael A ^a,b  

^a EUROPEAN MOLECULAR BIOLOGY LABORATORY   (Germany)

^b MAX PLANCK INSTITUTE FOR DEVELOPMENTAL BIOLOGY   (Germany)

^c RUHR UNIVERSITY BOCHUM   (Germany)

Author keywords

Biochemistry; Ca2+ phosphate transfection; Expression of foreign proteins; Primary hippocampal neurons; Western blot

Indexed keywords

CALCIUM PHOSPHATE; COMPLEMENTARY DNA; GLIAL FIBRILLARY ACIDIC PROTEIN;

ANIMAL CELL; ARTICLE; CELL SURVIVAL; CONTROLLED STUDY; DNA BINDING; EMBRYO; GENETIC TRANSFECTION; HIPPOCAMPUS; NERVE CELL; NONHUMAN; PRIORITY JOURNAL; PROTEIN EXPRESSION; RAT; TECHNIQUE;

ANIMALS; CELL CULTURE TECHNIQUES; GREEN FLUORESCENT PROTEINS; HIPPOCAMPUS; INDICATORS AND REAGENTS; LUMINESCENT PROTEINS; NEURONS; RATS; TRANSFECTION;

EID: 0344806277     PISSN: 03604012     EISSN: None     Source Type: Journal    
DOI: 10.1002/(SICI)1097-4547(19991215)58:6<831::AID-JNR10>3.0.CO;2-M     Document Type: Article

References (20)

1. Banker GA. 1980. Trophic interactions between astroglial cells and hippocampal neurons in culture. Science 209:809-810.
2. Banker GA, Cowan WM. 1977. Rat hippocampal neurons in dispersed cell culture. Brain Res 126:397-342.
3. Banker GA, Goslin K. 1998. Culturing nerve cells, 2nd ed. Cambridge, MA: MIT Press.
4. Blöchl A, Thoenen H. 1995. Characterization of nerve growth factor (NGF) release from hippocampal neurons: evidence for a constitutive and an unconventional sodium-dependent regulated pathway. Eur J Neurosci 7:1220-1228.
5. Craig AM. 1998. Transfecting cultured neurons. In: Banker G, Goslin K, editors. Culturing nerve cells, 2nd ed. Cambridge, MA: MIT Press. p 79-111.
6. De Hoop MJ, Meyn L, Dotti CG. 1998. Culturing hippocampal neurons and astrocytes from fetal rodent brain. In: Celis JE, editor. Cell biology: a laboratory handbook, 2nd ed. San Diego: Academic Press. p 154-163.
7. Dotti CG, Banker GA. 1987. Experimentally induced alteration in the polarity of developing neurons. Nature 330:254-256.
8. Dotti CG, Sullivan CA, Banker GA. 1988. The establishment of polarity by hippocampal neurons in culture. J Neurosci 8:1454-1468.
9. Goslin K, Banker G. 1998. Rat hippocampal neurons in low-density culture. In: Banker G, Goslin K, editors. Culturing nerve cells, 2nd ed. Cambridge, MA: MIT Press. p 339-370.
10. Graham FL, van der Eb AJ. 1973. A new technique for the assay of infectivity of human adenovirus 5 DNA. Virology 52:456-467.
11. Haubensak W, Narz F, Heumann R, Leßmann V. 1998. BDNF-GFP containing secretory granules are localized in the vicinity of synaptic junctions of cultured cortical neurons. J Cell Sci 111:1483-1493.
12. Holmes GL, Ben-Ari Y. 1998. Seizures in the developing brain: perhaps not so benign after all. Neuron 21:1231-1234.
13. Kaech S, Kim JB, Cariola M, Ralston E. 1996. Improved lipid-mediated gene transfer into primary cultures of hippocampal neurons. Mol Brain Res 35:344-348.
14. Kiebler M, Hemraj I, Verkade P, Köhrmann M, Fortes P, Marión RM, Ortín J, Dotti CG. 1999. The mammalian Staufen protein localizes to the somatodendritic domain of cultured hippocampal neurons: implications for its involvement in mRNA transport. J Neurosci 19:288-297.
15. Köhrmann M, Luo M, Kaether C, DesGroseillers L, Dotti CG, Kiebler MA. 1999. Microtubule-dependent recruitment of Staufen-GFP into large RNA-containing granules and subsequent dendritic transport in living hippocampal neurons. Mol Biol Cell 8:2945-2953.
16. Malinow R. 1998. Silencing the controversy in LTP? Neuron 21:1226-1227.
17. McEwen BS. 1999. Stress and hippocampal plasticity. Annu Rev Neurosci 22:105-122.
18. Nishi M, Takenaka N, Morita N, Ito T, Ozawa H, Kawata M. 1999. Real-time imaging of glucocorticoid receptor dynamics in living neurons and glial cells in comparison with non-neural cells. Eur J Neurosci 11:1927-1936.
19. Thomas A, Kim DS, Fields RL, Chin H, Gainer H. 1998. Quantitative analysis of gene expression in organotypic slice-explant cultures by particle-mediated gene transfer. J Neurosci Methods 84:181-191.
20. Xia Z, Dudek H, Miranti CK, Greenberg ME. 1996. Calcium influx via the NMDA receptor induces immediate early gene transcription by a MAP kinase/ERK-dependent mechanism. J Neurosci 16:5425-5436.