Expression of recombinant human acid sphingomyelinase in insect Sf21 cells: Purification, processing and enzymatic characterization

Journal of Biotechnology

Volumn 63, Issue 1, 1998, Pages 29-40

  Bartelsen, Oliver ^a   Lansmann, Stephanie ^a   Nettersheim, Michael ^a   Lemm, Thorsten ^a   Ferlinz, Klaus ^a   Sandhoff, Konrad ^a  

^a UNIVERSITY OF BONN   (Germany)

Author keywords

Acid sphingomyelinase; Baculovirus; Glycosylation; N terminal sequence; Proteolytic processing; Spodoptera frugiperda

Indexed keywords

BIOASSAY; CELLS; DEGRADATION; MICELLES; PROTEINS; PURIFICATION; VIRUSES;

BACULOVIRUS EXPRESSION VECTOR SYSTEMS; PROTEOLYTIC MATURATION; RECOMBINANT HUMAN ACID SPHINGOMYELINASE; SPODOPTERA FRUGIPERDA LARVAE;

ENZYME IMMOBILIZATION;

RECOMBINANT ENZYME; SPHINGOMYELIN PHOSPHODIESTERASE;

ANIMAL EXPERIMENT; ARTICLE; BACULOVIRUS; ENZYME ASSAY; ENZYME PURIFICATION; ENZYME STRUCTURE; ENZYME SYNTHESIS; EXPRESSION VECTOR; GLYCOSYLATION; LEPIDOPTERA; NONHUMAN; PRIORITY JOURNAL; PROTEIN DEGRADATION; PROTEIN EXPRESSION;

ANIMALS; BACULOVIRIDAE; CARBOHYDRATE SEQUENCE; CELLS, CULTURED; GLYCOPROTEINS; GLYCOSYLATION; HUMANS; KINETICS; MOLECULAR SEQUENCE DATA; OLIGOSACCHARIDES; PLACENTA; POLYSACCHARIDES; PROTEIN PRECURSORS; PROTEIN PROCESSING, POST-TRANSLATIONAL; RECOMBINANT PROTEINS; SEQUENCE ANALYSIS; SPHINGOMYELIN PHOSPHODIESTERASE; SPHINGOMYELINS; SPODOPTERA;

INSECTA; SPODOPTERA FRUGIPERDA; UNIDENTIFIED BACULOVIRUS;

EID: 0344701035     PISSN: 01681656     EISSN: None     Source Type: Journal    
DOI: 10.1016/S0168-1656(98)00070-4     Document Type: Article

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