Selective association of protein molecules followed by mass spectrometry

Protein Science

Volumn 8, Issue 6, 1999, Pages 1368-1370

  Hans, Vis ^a   Dobson, Christopher M ^a   Robinson, Carol V ^a  

^a UNIVERSITY OF OXFORD   (United Kingdom)

Author keywords

HU proteins; Nanoflow electrospray mass spectrometry; Protein interactions

Indexed keywords

BACTERIAL PROTEIN;

ARTICLE; DISSOCIATION; GEOBACILLUS STEAROTHERMOPHILUS; MASS SPECTROMETRY; MOLECULAR DYNAMICS; PRIORITY JOURNAL; PROTEIN PROTEIN INTERACTION; THERMODYNAMICS;

EID: 0344655671     PISSN: 09618368     EISSN: None     Source Type: Journal    
DOI: 10.1110/ps.8.6.1368     Document Type: Article

References (15)

1. Drlica K, Rouvière-Yaniv J. 1987. Histone-like proteins of bacteria. Microbiol Rev 51:301-119.
2. Groch N, Schindelin H, Scholtz AS, Hahn U, Heinemann U. 1992. Determination of DNA-binding parameters for the Bacillus subtilis histone-like HBsu protein through introduction of fluorophores by site-directed mutagenesis of a synthetic gene. Eur J Biochem 207:677-685.
3. Kawamura S, Kakuta Y, Tanaka I, Hikichi K, Kuhara S, Yamasaki N, Kimura M. 1996. Glycine-15 in the bend between two α-helices can explain the thermostability of DNA binding protein HU from Bacillus stearothermophilus. Biochemistry 35:1195-1200.
4. Nettleton EJ, Sunde M, Lai Z, Kelly JW, Dobson CM, Robinson CV. 1998. Protein subunit interactions and structural integrity of amyloidogenic transthyretins - Evidence from electrospray mass spectrometry. J Mol Biol 281:553-564.
5. Padas PM, Wilson KW, Vorgias CE. 1992. The DNA-binding protein HU from mesophilic and thermophilic bacilli: Gene cloning, overproduction, and purification. Gene 177:39-44.
6. Przybylski M, Glocker MO. 1996. Electrospray mass spectrometry of biomacromolecular complexes with noncovalent interactions - New analytical perspectives for supramolecular chemistry and molecular recognition processes. Angew Chem Int Ed Engl 35(8):806-826.
7. Rice PA. 1997. Making DNA do a U-turn: IHF and related proteins. Curr Opin Struct Biol 7(1):86-93.
8. Vis H, Heinemann U, Dobson CM, Robinson CV. 1998. Detection of a monomeric intermediate associated with dimerization of protein HU with mass spectrometry. J Am Chem Soc 120:6427-6428.
9. Vis H, Mariani M, Vorgias CE. Wilson KS, Kaptein R, Boelens R. 1995. Solution structure of the HU protein from Bacillus stearothermophilus. J Mol Biol 254:692-703.
10. Welfle H, Misselwitz R, Welfle K, Groch N, Heinemann U. 1992. Salt-dependent and protein-concentration-dependent changes in the solution structure of the DNA-binding histone-like protein, HBsu, from Bacillus subtilis. Eur J Biochem 204:1049-1055.
11. Welfe H, Welfle K, Misselwitz R, Groch N, Heinemann U. 1993. Conformational stability of the DNA-binding histone-like protein, HBsu, from Bacillus subtilis, and of the four variants [F29W], [F47W], [F50W], and [F79W]. J Biomol Struct Dynam 11(2):381-394.
12. White SW, Appelt K, Wilson KS, Tanaka I. 1989. A structural motif that bends DNA. Proteins 5:281-288.
13. Wilm MS, Mann M. 1994. Electrospray and Taylor - Cone theory, Dole's beam of macromolecules at last? Int J Mass Spectrom Ion Processes 136:167-180.
14. Wilm MS, Mann M. 1996. Analytical properties of the nano-electrospray ion source. Anal Chem 68(1):1-8.
15. Wilson KW, Vorgias CE, Tanaka I, White SW, Kimura M. 1990. The thermostability of DNA-binding protein HU from bacilli. Protein Eng 4:11-22.