Molecular cloning, expression and physical mapping of the human methionine synthase reductase gene

Gene

Volumn 240, Issue 1, 1999, Pages 75-88

  Leclerc, Daniel ^a   Odièvre, Marie Hélène ^a   Wu, Qing ^a   Wilson, Aaron ^a,b   Huizenga, Jack J ^c   Rozen, Rima ^a,b,d   Scherer, Stephen W ^c   Gravel, Roy A ^a,b,d  

^a RESEARCH INSTITUTE OF THE MCGILL UNIVERSITY HEALTH CENTRE   (Canada)

^b MCGILL UNIVERSITY HEALTH CENTRE   (Canada)

^c HOSPITAL FOR SICK CHILDREN   (Canada)

^d MCGILL UNIVERSITY   (Canada)

Author keywords

Chromosome 5; Cobalamin; Gene structure; Gene transcription; Reductive activation; Vitamin B12

Indexed keywords

METHIONINE SYNTHASE; OXIDOREDUCTASE;

ARTICLE; CAENORHABDITIS ELEGANS; CELL CULTURE; CELL STRAIN COS1; DNA LIBRARY; ENZYME ACTIVITY; GENE EXPRESSION; GENE MAPPING; HUMAN; IN VITRO STUDY; MOLECULAR CLONING; MYOTUBE; NONHUMAN; NUCLEOTIDE SEQUENCE; PRIORITY JOURNAL; PROMOTER REGION; REVERSE TRANSCRIPTION POLYMERASE CHAIN REACTION; SEQUENCE ANALYSIS; SEQUENCE HOMOLOGY;

ANIMALS; BASE SEQUENCE; CHLORAMPHENICOL O-ACETYLTRANSFERASE; CHROMOSOMES, HUMAN, PAIR 5; CLONING, MOLECULAR; COS CELLS; DNA; EXONS; EXPRESSED SEQUENCE TAGS; FERREDOXIN-NADP REDUCTASE; GENE EXPRESSION; GENES; HUMANS; HYBRID CELLS; INTRONS; MINISATELLITE REPEATS; MOLECULAR SEQUENCE DATA; PHYSICAL CHROMOSOME MAPPING; PROMOTER REGIONS (GENETICS); RECOMBINANT FUSION PROTEINS; SEQUENCE ANALYSIS, DNA; SEQUENCE DELETION; TRANSCRIPTION, GENETIC;

CAENORHABDITIS ELEGANS; EUKARYOTA;

EID: 0344603864     PISSN: 03781119     EISSN: None     Source Type: Journal    
DOI: 10.1016/S0378-1119(99)00431-X     Document Type: Article

References (29)

1. An G., Hidaka M., Siminovitch L. Expression of bacterial β-galactosidase in animal cells. Mol. Cell Biol. 2:1982;1628-1632.
2. Bianchi V., Reichard P., Eliasson R., Pontis E., Krook M., Jörnvall H., Haggård-Ljungquist E. Escherichia coli ferredoxin NADP+ reductase: activation of E. coli anaerobic ribonucleotide reduction, cloning of the gene (fpr) and overexpression of the protein. J. Bacteriol. 175:1993;1590-1595.
3. Chartrain N.A., Geller D.A., Koty P.P., Sitrin N.F., Nussler A.K., Hoffman E.P., Billiar T.R., Hutchinson N.I., Mudgett J.S. Molecular cloning, structure, and chromosomal localization of the human inducible nitric oxide synthase gene. J. Biol. Chem. 269:1994;6765-6772.
4. Church D.M., Yang J., Bocian M., Shiang R., Wasmuth J.J. A high-resolution physical and transcript map of the cri du chat region of human chromosome 5p. Genome Res. 7:1997;787-801.
5. Fan W., Christensen M., Eichler E., Zhang X., Lennon G. Cloning, sequencing, gene organization, and localization of the human ribosomal protein RPL23A gene. Genomics. 46:1997;234-239.
6. Gardiner-Garden M., Frommer M. CpG islands in vertebrate genomes. J. Mol. Biol. 196:1987;261-276.
7. Gulati S., Chen Z.Q., Brody L.C., Rosenblatt D.S., Banerjee R. Defects in auxiliary redox proteins lead to functional methionine synthase deficiency. J. Biol. Chem. 272:1997;19171-19175.
8. Heinemeyer T., Wingender E., Reuter I., Hermjakob H., Kel A.E., Kel O.V., Ignatieva E.V., Ananko E.A., Podkolodnaya O.A., Kolpakov F.A., Podkolodny N.L., Kolchanov N.A. Databases on transcriptional regulation: TRANSFAC, TRRD and COMPEL. Nucleic Acids Res. 26:1998;362-367.
9. Jarrett J.T., Hoover D.M., Ludwig M.L., Matthews R.G. The mechanism of adenosylmethionine-dependent activation of methionine synthase: a rapid kinetic analysis of intermediates in reductive methylationof Cob(II)alamin enzyme. Biochemistry. 37:1998;12649-12658.
10. Kingston R.E., Sheen J. Isotopic assays for reporter gene activity. Current Protocols in Molecular Biology. 1998;9.7.1-9.7.11 Wiley, New York.
11. Kollmar R., Sukow K.A., Sponagle S.K., Farnham P.J. Start site selection at the TATA-less carbamoyl-phosphate synthase (glutamine-hydrolyzing)/aspartate carbamoyltransferase/dihydroorotase promoter. J. Biol. Chem. 269:1994;2252-2257.
12. Kozak M. Structural features in eukaryotic mRNAs that modulate the initiation of translation. J. Biol. Chem. 266:1991;19867-19870.
13. Kriegler M. Gene Transfer and Expression. 1990;Stockton Press, New York.
14. Leclerc D., Wilson A., Dumas R., Gafuik C., Song D., Watkins D., Heng H.H.Q., Rommens J.M., Scherer S.W., Rosenblatt D.S., Gravel R.A. Cloning and mapping of a cDNA for methionine synthase reductase, a flavoprotein defective in patients with homocystinuria. Proc. Natl. Acad. Sci. USA. 95:1998;3059-3064.
15. Luckow B., Schütz G. CAT constructions with multiple unique restriction sites for the functional analysis of eukaryotic promoters and regulatory elements. Nucleic Acids Res. 15:1987;5490.
16. MacGregor G.R., Caskey C.T. Construction of plasmids that express E. coli beta-galactosidase in mammalian cells. Nucleic Acids Res. 17:1989;2365.
17. McEwen D.G., Ornitz D.M. Regulation of the fibroblast growth factor receptor 3 promoter and intron 1 enhancer by SP1 family transcription factors. J. Biol. Chem. 273:1988;5349-5357.
18. Marsden P.A., Heng H.H.Q., Scherer S.W., Stewart R., Hall A.V., Shi W.M., Tsui L.C., Schappert K.T. Structure and chromosomal localization of the human constitutive endothelial nitric oxide synthase gene. J. Biol. Chem. 268:1993;17478-17488.
19. Means A.L., Farnham P.J. Transcription initiation from the dihydrofolate reductase promoter is positioned by HIP1 binding at the initiation site. Mol. Cell. Biol. 10:1990;653-661.
20. Osborne C., Chen L.M., Matthews R.G. Isolation, cloning, mapping, and nucleotide sequencing of the gene encoding flavodoxin in Escherichia coli. J. Bacteriol. 173:1991;1729-1737.
21. Porter T.D., Beck T.W., Kasper C.B. NADPH-cytochrome P-450 oxidoreductase gene organization correlates with structural domains of the protein. Biochemistry. 29:1990;9814-9818.
22. Proudfoot N.J., Whitelaw E. Termination and 3′end processing of eukaryotic RNA. Transcription and Splicing. 1988;97 IRL Press, Oxford.
23. Rosenblatt D.S., Cooper B.A., Pottier A., Lue-Shing H., Matiaszuk N., Grauer K. J. Clin. Invest. 74:1984;2149-2156.
24. Rozen R. Molecular genetic aspects of hyperhomocysteinemia and its relation to folic acid. Clin. Invest. Med. 19:1996;171-178.
25. Schug J., Overton C. TESS: Transcription elements search software on the WWW. Technical Report CBIL-TR-1997-1001-V0.0 of the Computational Biology and Informatics Laboratory, School of Medicine, University of Pennsylvania. 1997.
26. Shoubridge E.A., Johns T., Boulet L. Use of myoblast cultures to study mitochondrial myopathies. Meth. Enzymol. 264:1996;465-475.
27. Genome sequence of the nematode C. elegans: a platform for investing biology. Science. 282:1998;2012-2018.
28. Tone M., Diamond L.E., Walsh L.A., Tone Y., Thompson S.A.J., Shanahan E.M., Logan J.S., Waldmann H. High level transcription of the complement regulatory protein CD59 requires an enhancer located in intron 1. J. Biol. Chem. 274:1999;710-716.
29. 12) increases risk for Spina Bifida. Mol. Genet. Metab. 67:1999;317-323.