A modified enzyme-linked immunosorbent assay for detection of cutaneous antibody against Ichthyophthirius multifiliis

Bulletin of the European Association of Fish Pathologists

Volumn 23, Issue 5, 2003, Pages 228-234

  Xu, De Hai ^a   Klesius, Phillip H ^a   Shelby, Richard A ^a  

^a AQUATIC ANIMAL HEALTH RESEARCH UNIT   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

ANIMALIA; ARMORACIA RUSTICANA; CATFISH; ICHTHYOPHTHIRIUS MULTIFILIIS; ICTALURUS PUNCTATUS;

EID: 0344586818     PISSN: 01080288     EISSN: None     Source Type: Journal    
DOI: None     Document Type: Article

References (15)

1. Becker, H. and Allison, R. (1964) Some host responses of white catfish to Ichthyophthirius multifiliis (Fouquet). Proc. Southeastern Assoc. Game Fish Commiss. 18:438-441.
2. Clark, T.G., Dickerson, H.W. and Findly, R.C. (1988) Immune response of channel catfish to ciliary antigens of Ichthyophthirius multifiliis. Dev. Comp. Immunol. 12:581-594.
3. Clark T.G., Dickerson H.W., Gratzek J.B., and Findly R.C. (1987) In vitro response of Ichthyophthirius multifiliis to sera from immune channel catfish. J. Fish Biol. 31 (Suppl. A):203-208.
4. Dickerson, H. and Clark, T. (1998) Ichthyophthirius multifiliis: a model of cutaneous infection and immunity in fishes. Immunol. Reviews 166:377-384.
5. Goka, A.K. and Farthing, M.J. (1987) The use of 3,3′,5,5′- tetramethylbenzidine as a peroxidase substrate in microplate enzyme-liked immunosorbent assay. J. Immunoassay 8:29-41.
6. Hines R.S. and Spira, D.T. (1974) Ichthyophthiriasis in the mirror carp carpio (L.) V. acquired immunity. J. Fish Biol. 6:373-378.
7. Jessop, B.M. (1995) Ichthyophthirius multifiliis in elvers and small American eels from the East River, Nova Scotia. J. Aquat. Anim. Health 7:54-57.
8. Lin T.L., Clark T.G. and Dickerson H. (1996) Passive immunization of channel catfish (Ictalurus punctatus) against the ciliated protozoan parasite Ichthyophthirius multifiliis by use of murine monoclonal antibodies. Infection and Immunity 64:4085-4091.
9. SAS Institute (1989) SAS/Stat user's guide, Version 6, Fourth edition. SAS Institute, Cary, North Carolina.
10. Sigh, J. and Buchmann, K. (2001) Comparison of immobilization assays and enzyme-linked immunosorbent assays for detection of rainbow trout antibody-titers against Ichthyophthirius multifiliis Fouquet, 1876. J. Fish Dis. 24:49-51.
11. Traxler, G.S., Richard, J. and McDonald, T.E. (1998) Ichthyophthirius multifiliis (Ich) epizootics in spawning sockeys salmon in British Columbia, Canada. J. Aquat. Anim. Health 10:143-151.
12. Wang, X. and Dickerson, H.W. (2002) Surface immobilization antigen of the parasite ciliate Ichthyophthirius multifiliis elicits protective immunity in channel catfish (Ictalurus punctatus). Clin. Diag. Lab. Immunol. 9:176-181.
13. Xu, D.H. and Klesius, P.H. (2002) Antibody mediated immune response against Ichthyophthirius using excised skin from channel catfish immune to Ichthyophthirius. J. Fish Dis. 25:299-306.
14. Xu, D.H., Klesius, P.H. and Shelby, R.A. (2002) Cutaneous antibodies in excised skin from channel catfish, Ictalurus punctatus Rafinesque, immune to Ichthyophthirius multifiliis. J. Fish Dis. 25:45-52.
15. Volpe, G., Compagnone, D., Draisci, R. and Palleschi, G. (1998) 3,3′,5,5′-Tetramethylbenzidine as electrochemical substrate for horseradish peroxidase based enzyme immunoassays. A comparative study. Analyst 123:1303-1307.