Validation of a rapid method for extraction of total RNA applied to Leishmania promastigotes

Journal of Parasitology

Volumn 85, Issue 4, 1999, Pages 757-758

  Perez J A ^a   Aragon Z ^a   Pinero J E ^a   Del Castillo, A ^a   De Armas, F ^a   Valladares, B ^a  

^a UNIVERSIDAD DE LA LAGUNA   (Spain)

Author keywords

[No Author keywords available]

Indexed keywords

PHENOL; PROTOZOAL RNA;

ARTICLE; EXTRACTION; LEISHMANIA; NONHUMAN; REVERSE TRANSCRIPTION POLYMERASE CHAIN REACTION; RNA ANALYSIS;

ANIMALS; LEISHMANIA; REVERSE TRANSCRIPTASE POLYMERASE CHAIN REACTION; RNA, PROTOZOAN;

EID: 0344564201     PISSN: 00223395     EISSN: None     Source Type: Journal    
DOI: 10.2307/3285759     Document Type: Article

References (5)

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2. CHOMCZYNSKI, P., AND N. SACCHI. 1987. Single-step method of RNA isolation by acid guanidinium thiocyanate-phenol-chloroform extraction. Analytical Biochemistry 162: 156-159.
3. COLLART, M. S., AND S. OLIVEIRO. 1993. Preparation of yeast RNA. In Current protocols in molecular biology, F. M. Ausubel, R. Brent, R. E. Kingston, D. D. Moore, J. G. Seidman, J. A. Smith, and K. Struhl (eds.). Greene Publishing Associates and Wiley-Interscience, New York, New York, p. 13.12.1-13.12.5.
4. SAMBROOK, J., E. F. FRITSCH, AND T. MANIATIS. 1989. Molecular cloning: A laboratory manual, 2nd ed. CSH Laboratory Press, Cold Spring Harbor, New York.
5. YANCOPOULOS, G. D., AND F. ALT. 1990. Preparation of cytoplasmic RNA. In Methods for cloning and analysis of eukaryotic genes, A. Bothwell, G. D. Yancopoulos, and F. Alt (eds.). Jones and Bartlett Publishers, Boston, Massachusetts, p. 18.