Pantothenate production in Escherichia coli K12 by enhanced expression of the panE gene encoding ketopantoate reductase

Journal of Biotechnology

Volumn 75, Issue 2-3, 1999, Pages 135-146

  Elischewski, Frank ^a   Pühler, Alfred ^a   Kalinowski, Jörn ^a  

^a BIELEFELD UNIVERSITY   (Germany)

Author keywords

Gene replacement; Tn5 mutagenesis; Vitamins

Indexed keywords

AMINO ACIDS; ENZYME KINETICS; ENZYMES; ESCHERICHIA COLI; GROWTH KINETICS; MUTAGENESIS; PROTEINS; VITAMINS;

GENE REPLACEMENT; GENETIC COMPLEMENTATION; KETOPANTOATE REDUCTASE; PANTOTHENATE;

GENETIC ENGINEERING;

BACTERIAL ENZYME; OXIDOREDUCTASE;

ARTICLE; BACTERIAL GENE; BACTERIAL METABOLISM; BACTERIUM MUTANT; ENZYME ACTIVITY; ENZYME ANALYSIS; ESCHERICHIA COLI K 12; GENE EXPRESSION; GENETIC COMPLEMENTATION; NONHUMAN; PLASMID; PRIORITY JOURNAL;

ALCOHOL OXIDOREDUCTASES; AMINO ACID SEQUENCE; BASE SEQUENCE; CLONING, MOLECULAR; ESCHERICHIA COLI; GENE EXPRESSION REGULATION, BACTERIAL; GENETIC COMPLEMENTATION TEST; MOLECULAR SEQUENCE DATA; MUTATION; PANTOTHENIC ACID;

EID: 0344549464     PISSN: 01681656     EISSN: None     Source Type: Journal    
DOI: 10.1016/S0168-1656(99)00153-4     Document Type: Article

References (26)

1. Altschul S.F., Gish W., Miller W., Myers E.W., Lipman D.J. Basic local alignment search tool. J. Mol. Biol. 215:1990;403-410.
2. Appleyard R.K. Segregation of new lysogenic types during growth of a doubly lysogenic strain derived from Escherichia coli K12. Genetics. 39:1954;440-452.
3. Becker A., Schmidt M., Jäger W., Pühler A. New gentamicin-resistance and lacZ promoter-probe cassettes suitable for insertion mutagenesis and generation of transcriptional fusions. Gene. 162:1995;37-39.
4. Berlyn, M.K.B., Low, K.B., Rudd, K.E., 1996. In: Neidhardt, F.C., et al. (Eds.), Escherichia coli and Salmonella, 2nd ed., ASM Press, Washington D.C., pp. 1715-1902.
5. Brosius J., Holy A. Regulation of ribosomal RNA promoters with a synthetic lac operator. Proc. Natl. Acad. Sci. USA. 81:1984;6929-6933.
6. Downs D.M., Petersen L. apbA, a new genetic locus involved in thiamine biosynthesis in Salmonella Typhimurium. J. Bacteriol. 176:1994;4858-4864.
7. Fellay R., Frey J., Kirsch H. Interposon mutagenesis of soil and water bacteria: a family of DNA fragments designed for in vitro insertional mutagenesis of Gram-negative bacteria. Gene. 52:1987;147-154.
8. Frodyma M.E., Downs D. ApbA, the ketopantoate reductase enzyme of Salmonella typhimurium is required for the synthesis of thiamine via the alternative pyrimidine biosynthetic pathway. J. Biol. Chem. 273:1998;5572-5576.
9. Frodyma M.E., Downs D. The panE gene, encoding ketopantoate reductase, maps at 10 minutes and is allelic to apbA in Salmonella typhimurium. J. Bacteriol. 180:1998;4757-4759.
10. Frost J.W., Bender J.L., Kadonaga J.T., Knowles J.R. Dehydroquinate synthase from Escherichia coli: purification, cloning, and construction of overproducers of the enzyme. Biochemistry. 23:1984;4470-4475.
11. Grant S.G.N., Jessee J., Bloom F.R., Hanahan D. Differential plasmid rescue from transgenic mouse DNAs into Escherichia coli methylation-restriction mutants. Proc. Natl. Acad. Sci. USA. 87:1990;4645-4649.
12. Hagemann, R., 1990. Gentechnologische Arbeitsmethoden. Gustav Fischer Verlag, Stuttgart, New York.
13. Hamilton C.M., Aldea M., Washburn B.K., Babitzke P., Kushner S.R. New method for generating deletions and gene replacements in Escherichia coli. J. Bacteriol. 171:1989;4617-4622.
14. Innis M.A., Gelfand D.H., Sninsky J.J., White T.J. PCR Protocols: A Guide to Methods and Applications. 1990;Academic Press, San Diego, CA.
15. Jackowski, S, 1996. In: Neidhardt, F.C., et al. (Eds.), Escherichia coli and Salmonella, 2nd ed., ASM Press, Washington, D.C., pp. 687-694.
16. King J., Laemmli U.K. Polypeptides of the tail fibres of bacteriophage T4. J. Mol. Biol. 62:1971;465-477.
17. Primerano D.A., Burns R.O. Role of acetohydroxy acid isomeroreductase in biosynthesis of pantothenic acid in Salmonella typhimurium. J. Bacteriol. 153:1983;259-269.
18. Sambrook J., Fritsch E.F., Maniatis T. Molecular Cloning: A Laboratory Manual. 2nd edn:1989;Cold Spring Harbor Laboratory, Cold Spring Harbor, NY.
19. Shimizu S., Kataoka M., Chung M.C.-M., Yamada H. Ketopantoic acid reductase of Pseudomonas maltophilia 845. J. Biol. Chem. 263:1988;12077-12084.
20. Simon R., Quandt J., Klipp W. New derivatives of transposon Tn5 suitable for mobilization of replicons, generation of operon fusions and induction of genes in gram-negative bacteria. Gene. 80:1989;161-169.
21. TAKEDA, 1991. European Patent Application, no. 0493060A2.
22. TAKEDA, 1993. European Patent Application, no. 0590857A.
23. TAKEDA, 1997. International Patent Application, no. WO 97/10340.
24. Tauch A., Kassing O., Wehmeier L., Kalinowski J., Pühler A. Corynebacterium glutamicum DNA is subjected to methylation-restriction in Escherichia coli. FEMS Microbiol. Lett. 123:1994;343-347.
25. Vogel H.J., Bonner D.M. Acetylornithinase of Escherichia coli: partial purification and some properties. J. Biol. Chem. 218:1956;97-106.
26. Wilken D.R., King H.L., Dyar R.E. Ketopantoic acid and ketopantoyl lactone reductases. J. Biol. Chem. 250:1975;2311-2314.