Purification and preliminary crystallographic studies on the sporulation response regulatory phosphotransferase protein, Spo0B, from Bacillus subtilis

Proteins: Structure, Function and Genetics

Volumn 27, Issue 4, 1997, Pages 597-600

  Zhou, Xiao Zhen ^a   Madhusudan, ^a   Whiteley, J M ^a   Hoch, J A ^a   Varughese, K I ^a  

^a SCRIPPS RESEARCH INSTITUTE   (United States)

Author keywords

crystallization; phosphotransferase; signal transduction

Indexed keywords

PHOSPHOTRANSFERASE;

ARTICLE; BACILLUS SUBTILIS; ESCHERICHIA COLI; NONHUMAN; PRIORITY JOURNAL; SIGNAL TRANSDUCTION; SPOROGENESIS; X RAY CRYSTALLOGRAPHY;

BACILLUS SUBTILIS; BACTERIAL PROTEINS; CRYSTALLOGRAPHY, X-RAY; PHOSPHOTRANSFERASES; RECOMBINANT PROTEINS; SIGMA FACTOR; SIGNAL TRANSDUCTION; SPORES, BACTERIAL; SYNCHROTRONS; TRANSCRIPTION FACTORS;

BACILLUS SUBTILIS; ESCHERICHIA COLI;

EID: 0344532884     PISSN: 08873585     EISSN: None     Source Type: Journal    
DOI: 10.1002/(SICI)1097-0134(199704)27:4<597::AID-PROT11>3.0.CO;2-F     Document Type: Article

References (11)

1. Chang, C., Kwok, S.F., Bleecker, A.B., Meyerowitz, E.M. Arabidopsis ethylene-response gene etrI: Similarity of product to two-component regulators. Science 262:539-544, 1993.
2. Ninfa, A.J., Magasanik, B. Covalent modification of the glnG product, NRI, by the glnL product, NRII, regulates the transcription of the glnALG operon in Escherichia coli. Proc. Natl. Acad. Sci. USA 83:5909-5913, 1986.
3. Parkinson, J.S., Kofoid, B.C. Communication modules in bacterial signaling proteins. Annu. Rev. Genet. 26:71-112, 1992.
4. Burbulys, D., Trach, K.A., Hoch, J.A. The initiation of sporulation in Bacillus subtilis is controlled by a multicomponent phosphorelay. Cell 64:545-552, 1991.
5. Ferrari, F.A., Trach, K., Hoch, J.A. Sequence analysis of the SpoOB locus reveals a polycistronic transcription unit. J. Bacteriol. 161:556-562, 1985.
6. Uhl, M.A., Miller, J.F. Integration of multiple domains in a two-component sensor protein: The Bordetella pertussis BvgAS phosphorelay. EMBO J. 15:1028-1036, 1996.
7. Madhusudan, , Zapf, J., Whiteley, J.M., Hoch, J.A., Xuong, N.H., Varughese, K.I. Crystal structure of a phosphatase-resistant mutant of sporulation response regulator SpoOF from Bacillus subtilis. Structure 4:679-690, 1996.
8. Mathews, B. Solvent content of protein crystals. J. Mol. Biol. 33:491-497, 1968.
9. Stock, J.B., Surette, M.G., Levit, M. and Park, P. Two-component signal transduction systems: Structure-function relationships and mechanisms of catalysis. In: "Two-Component Signal Transduction." Hoch, J.A., Silhavy, T.J. (eds.). Washington, DC: ASM Press, 1995, pp. 25-51.
10. II two-component system of enteric bacteria. In: "Two-Component Signal Transduction." Hoch, J.A., Silhavy, T.J. (eds.). Washington, DC: ASM Press, 1995, pp. 67-88.
11. Otwinowski, Z. "DENZO: A Program for Automatic evaluation of Film Densities." New Haven, CT: Yale University Press, 1988.