The propeptide in the precursor form of carboxypeptidase Y ensures cooperative unfolding and the carbohydrate moiety exerts a protective effect against heat and pressure

European Journal of Biochemistry

Volumn 270, Issue 22, 2003, Pages 4587-4593

  Kato, Michiko ^a   Sato, Yasuhiro ^a   Shirai, Kumiko ^a   Hayashi, Rikimaru ^a,c   Balny, Claude ^b   Lange, Reinhard ^b  

^a KYOTO UNIVERSITY   (Japan)

^b INSERM   (France)

^c Nihon University   (Japan)

Author keywords

Carboxypeptidase Y; Fluorescence spectrometry; Pressure unfolding; Procarboxypeptidase Y; Thermal unfolding

Indexed keywords

AMINO ACID; CARBOHYDRATE; CARBOXYPEPTIDASE C; ENZYME PRECURSOR; PEPTIDE;

ARTICLE; CARBOHYDRATE ANALYSIS; DIFFERENTIAL SCANNING CALORIMETRY; FLUORESCENCE SPECTROSCOPY; HEAT; PRESSURE; PRIORITY JOURNAL; PROTEIN ANALYSIS; PROTEIN DOMAIN; PROTEIN FOLDING; PROTEIN GLYCOSYLATION; PROTEIN STRUCTURE; TEMPERATURE;

CALORIMETRY, DIFFERENTIAL SCANNING; CARBOHYDRATE METABOLISM; CARBOHYDRATES; CARBOXYPEPTIDASE C; GLYCOSYLATION; HEAT; MODELS, MOLECULAR; PRESSURE; PROTEIN DENATURATION; PROTEIN FOLDING; PROTEIN PRECURSORS; PROTEIN STRUCTURE, SECONDARY; SACCHAROMYCES CEREVISIAE; SACCHAROMYCES CEREVISIAE PROTEINS;

EID: 0344442879     PISSN: 00142956     EISSN: None     Source Type: Journal    
DOI: 10.1046/j.1432-1033.2003.03860.x     Document Type: Article

References (29)

1. Hayashi, R. (1976) Carboxypeptidase Y. Methods Enzymol. 45, 568-587.
2. Valls, L.A., Hunter, C.P., Rothman, J.H. & Stevens, T.H. (1987) Protein sorting in yeast: The localization determinant of yeast vacuolar carboxypeptidase Y residues in the propeptide. Cell. 48, 887-897.
3. Jung, G., Ueno, H. & Hayashi, R. (1999) Carboxypeptidase Y: Structural basis for protein sorting and catalytic triad. J. Biochem. 126, 1-6.
4. Ramos, C., Winther, J.R. & Kielland-Brandt, M.C. (1994) Requirement of the propeptide for in vivo formation of active yeast carboxypeptidase Y. J. Biol. Chem. 269, 7006-7012.
5. Winther, J.R. & Sørensen, P. (1991) Propeptide of carboxypeptidase Y provides a chaperonin-like function as well as inhibition of enzymatic activity. Proc. Natl Acad. Sci. USA 88, 9330-9334.
6. Winther, J.R., Sørensen, P. & Kielland-Brandt, M.C. (1994) Refolding of a carboxypeptidase Y folding intermediate in vitro by low-affinity binding of the proregion. J. Biol. Chem. 269, 22007-22013.
7. Hata, T., Hayashi, R. & Doi, E. (1967) Purification of yeast proteinases. Part III. Isolation and physicochemical properties of yeast proteinase A and C. Agric. Biol. Chem. 31, 357-367.
8. Aibara, S., Hayashi, R. & Hata, T. (1971) Physical and chemical properties of yeast proteinase C. Agric. Biol. Chem. 35, 658-666.
9. Endrizzi, J.A., Breddam & K., Remington, S.J. (1994) 2.8-Å structure of yeast serine carboxypeptidase Y. Biochemistry 33, 11106-11120.
10. Hasilik, A. & Tanner, W. (1978) Biosynthesis of the vacuolar yeast glycoprotein carboxypeptidase Y. Eur. J. Biochem. 85, 599-608.
11. Hashimoto, C., Cohen, R.E., Zhang, W.-J. & Ballou, C.E. (1981) Carbohydrate chains on yeast carboxypeptidase Y are phosphorylated. Proc. Natl Acad. Sci. USA 78, 2244-2248.
12. Trimble, R.B., Maley, F. & Chu, F.K. (1983) Glycoprotein biosynthesis in yeast. J. Biol. Chem. 258, 2562-2567.
13. Shimizu, H., Ueno, H. & Hayashi, R. (1999) Role of carbohydrate moiety in carboxypeptidase Y: Structural study of mutant enzyme lacking carbohydrate moiety. Biosci. Biotechnol. Biochem. 63, 1045-1050.
14. Zhou, J.M., Zhu, L. & Balny, C. (2000) Inactivation of creatine kinase by high pressure may precede dimer dissociation. Eur. J. Biochem. 267, 1247-1253.
15. Ruan, K., Xu, C., Yu, Y., Li, J., Lange, R., Bec, N. & Balny, C. (2003) The thermodynamic analysis of protein stabilization by sucrose and glycerol against pressure-induced unfolding. Eur. J. Biochem. 270, 1654-1661.
16. Ruan, K., Xu, C., Yu, Y., Li, J., Lange, R., Bec, N. & Balny, C. (2001) Pressure-exploration of the 33-kDa protein from spinach photosystem II particle. Eur. J. Biochem. 268, 2742-2750.
17. Ruan, K., Lange, R., Bec, N. & Balny, C. (1997) A stable partly denatured state of trypsin induced by high hydrostatic pressure. Biochem. Biophys. Res. Commun. 239, 150-154.
18. Torrent, J. (2003) Alternative prion structural changes revealed by high pressure. Biochemistry 42, 1318-1325.
19. Hamaguchi, K. (1992) Physico-Chemical Properties of Amino Acid Chains. Japan Scientific Society Press, Tokyo.
20. Bee, N., Villa, A., Tortora, P., Mozhaev, V.V., Balny, C. & Lange, R. (1996) Enhanced stability of carboxypeptidase from Sulfolobus solfataricus at high pressure. Biotechnol. Lett. 18, 482-488.
21. Lange, R., Frank, J., Saldana, J.-L. & Balny, C. (1996) Fourth derivative UV-spectroscopy of proteins under high pressure. I. Factors affecting the fourth derivative spectrum of the aromatic amino acids. Eur. Biophys. J. 24, 277-283.
22. Gibson, R.E. & Loeffler, O.H. (1941) Pressure-volume-temperature relations in solutions. V. The energy-volume coefficients of the carbon tetrachloride, water and ethylene glycol. J. Am. Chem. Soc. 63, 898-906.
23. Neuman, R.C. Jr., Kauzmann, W. & Zipp, A. (1973) Pressure dependence of weak acid ionization in aqueous buffers. J. Phys. Chem. 77, 2687-2691.
24. Silva, J., Miles, E. & Weber, G. (1986) Pressure dissociation and conformational drift of the β dimer of tryptophane synthase. Biochemistry 25, 5780-5786.
25. Dumoulin, M., Ueno, H., Hayashi, R. & Balny, C. (1999) Contribution of the carbohydrate moiety to conformational stability of the carboxypeptidase Y: High pressure study. Eur. J. Biochem. 262, 475-483.
26. Kunugi, S., Yanagi, Y., Kitayaki, M., Tanaka, N. & Uehara-Kunugi, Y. (1997) Effects of high-pressure on the activity and spectroscopic properties of carboxypeptidase Y. Bull. Chem. Soc. Jpn. 70, 1459-1463.
27. Masson, P. & Cléry, C. (1996) Pressure-induced molten globule states of proteins. In High Pressure Bioscience and Biotechnology (Hayashi, R. & Balny, C., eds), pp. 117-126. Elsevier Science B.V., the Netherlands.
28. Ptitsyn, O.B. (1995) Molten globule and protein folding. Adv. Prot. Chem. 47, 83-229.
29. Trovaslet, M., Dallet-Choisy, S., Meersman, F., Heremans, K., Balny, C. & Legoy, M.-D. (2003) Fluorescence and FTIR study of pressure-induced structural modifications of horse liver alcohol dehydrogenase (HLADH). Eur. J. Biochem. 270, 119-128.