Calcium mobilization by ryanodine promotes the phosphorylation of initiation factor 2α subunit and inhibits protein synthesis in cultured neurons

Journal of Neurochemistry

Volumn 69, Issue 4, 1997, Pages 1703-1708

  Alcáar, Alberto ^a   De La Vega, Cristina Martín ^a   Bazán, Eulalia ^a   Fando, Juan L ^b   Salinas, Matilde ^a  

^a HOSPITAL RAMÓN Y CAJAL   (Spain)

^b UNIVERSITY OF ALCALÁ   (Spain)

Author keywords

Cultured neurons; Double stranded RNA dependent eukaryotic initiation factor 2 kinase; Initiation factor 2 phosphorylation; Intracellular calcium regulation; Protein synthesis; Ryanodine

Indexed keywords

CALCIUM ION; CARBACHOL; GLUTAMIC ACID; INITIATION FACTOR 2; INOSITOL 1,4,5 TRISPHOSPHATE RECEPTOR; PROTEIN SUBUNIT; RYANODINE; RYANODINE RECEPTOR;

ANIMAL CELL; ARTICLE; CALCIUM CELL LEVEL; CALCIUM MOBILIZATION; CELL VIABILITY; CONTROLLED STUDY; ENZYME ACTIVITY; NONHUMAN; PRIORITY JOURNAL; PROTEIN PHOSPHORYLATION; PROTEIN SYNTHESIS; RAT; RNA TRANSLATION;

ANIMALIA; EUKARYOTA;

EID: 0344369823     PISSN: 00223042     EISSN: None     Source Type: Journal    
DOI: 10.1046/j.1471-4159.1997.69041703.x     Document Type: Article

References (31)

1. 2+ ionophore-treated cultured neuronal cells. Neurosci. Lett. 201, 215-218.
2. Alcázar A., Martín M. E., Soria E., Rodríguez S., Fando J. L., and Salinas M. (1995b) Purification and characterization of guanine nucleotide-exchange factor, eIF-2B, and p37 calmodulin-binding protein from calf brain. J. Neurochem. 65, 754-761.
3. Alcázar A., Rivera J., Gómez-Calcerrada M., Muñoz F., Salinas M., and Fando J. L. (1996) Changes in the phosphorylation of eukaryotic initiation factor 2α, initiation factor 2B activity and traslational rates in primary neuronal cultures under different physiological growing conditions. Mol. Brain Res. 38, 101-108.
4. Bradford M. M. (1976) A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding. Anal. Biochem. 72, 248-254.
5. Brostrom C. O. and Brostrom M. A. (1990) Calcium-dependent regulation of protein synthesis in intact mammalian cells. Annu. Rev. Physiol. 52, 577-590.
6. Browning K. S., Humphreys J., Hobbs W., Smith G. B., and Ravel J. M. (1990) Determination of the amounts of the protein synthesis initiation and elongation factors in wheat germ. J. Biol. Chem. 265, 17967-17973.
7. Carter A. J. and Müller R. E. (1991) Activation of excitatory amino acid receptors cannot alone account for anoxia-induced impairment of protein synthesis in rat hippocampal slices. J. Neurochem. 57, 888-896.
8. Chavis P., Fagni L., Lansman J. B., and Bockaert J. (1996) Functional coupling between ryanodine receptors and L-type calcium channels in neurons. Nature 382, 719-722.
9. Clapham D. E. (1995) Calcium signalling. Cell 80, 259-268.
10. Dux E., Oschlies U., Wiessner C., and Hossmann K. A. (1992) Glutamate-induced ribosomal disaggregation and ultrastructural changes in rat cortical neuronal culture: protective effect of horse serum. Neurosci. Lett. 141, 173-176.
11. 2+-release channels. Trends Pharmacol. Sci. 15, 145-149.
12. García A. M., Martín M. E., Alcázar A., Fando J. L., and Salinas M. (1994) Protein synthesis in the developing rat liver: participation of initiation factors eIF-2 and eIF-2B. Hepatology 20, 706-713.
13. Ghosh A. and Greenberg M. E. (1995) Calcium signalling in neurons: molecular mechanisms and cellular consequences. Science 268, 239-247.
14. Grynkiewicz G., Poenie M., and Tsien R. Y. (1985) A new generation of calcium indicators with greatly improved fluorescence properties. J. Biol. Chem. 260, 3440-3450.
15. 2+ in neurons. Neuroscience 46, 251-273.
16. 2+, and cell growth. Am. J. Physiol. 258, C533-C543.
17. Kimball S. R. and Jefferson L. S. (1990) Mechanism of the inhibition of protein synthesis by vasopressin in rat liver. J. Biol. Chem. 265, 16794-16798.
18. Koch R. A. and Barish M. E. (1994) Pertubation of intracellular calcium and hydrogen ion regulation in cultured mouse hippocampal neurons by reduction of the sodium ion concentration gradient. J. Neurosci. 14, 2585-2593.
19. Mellor H., Flowers K. M., Kimball S. R., and Jefferson L. S. (1994) Cloning and characterization of a cDNA encoding rat PKR, the double-stranded RNA-dependent eukaryotic initiation factor-2 kinase. Biochim. Biophys. Acta 1219, 693-696.
20. Menéndez N., Solís J. M., Herreras O., Galarreta M., Conejero C., and Martín del Río R. (1993) Taurine release evoked by NMDA receptor activation is largely dependent on calcium mobilization from intracellular stores. Eur. J. Neurosci. 5, 1273-1279.
21. Pain V. M. (1996) Initiation of protein synthesis in eukaryotic cells. Eur. J. Biochem. 236, 747-771.
22. Petryshyn R., Chen J. J., and London I. M. (1988) Detection of activated double-stranded RNA-dependent protein kinase in 3T3-F442A cells. Proc. Natl. Acad. Sci. USA 85, 1427-1431.
23. Prostko C. R., Dholakia J. N., Brostrom M. A., and Brostrom C. O. (1995) Activation of the double-stranded RNA-regulated protein kinase by depletion of endoplasmic reticular calcium stores. J. Biol. Chem. 270, 6211-6215.
24. Proud C. G. (1992) Protein phosphorylation in translational control. Curr. Top. Cell Regul. 32, 243-386.
25. Proud C. G. (1995) PKR: a new name and new roles. Trends Pharmacol Sci. 20, 241-246.
26. Ranu R. S. and London I. M. (1979) Regulation of protein synthesis in rabbit reticulocyte lysates: preparation of efficient protein synthesis lysates and the purification and characterization of the heme-regulated translational inhibitory protein kinase. Methods Enzymol. 60, 459-484.
27. 2+ stores: activation and function. Trends Neurosci. 18, 299-306.
28. Srivastava S. P., Davies M. V., and Kaufman R. J. (1995) Calcium depletion from the endoplasmic reticulum activates the double-stranded RNA-dependent protein kinase (PKR) to inhibit protein synthesis. J. Biol. Chem. 270, 16619-16624.
29. Szatkowski M. and Attwell D. (1994) Triggering and execution of neuronal death in brain ischaemia: two phases of glutamate release by different mechanisms. Trends Neurosci. 17, 359-365.
30. Vornov J. J. and Coyle J. T. (1991) Glutamate neurotoxicity and the inhibition of protein synthesis in the hippocampal slice. J. Neurochem. 56, 996-1006.
31. Weiss S., Schmidt B. H., Sebben M., Kemp D. E., Bockaert J., and Sladeczek F. (1988) Neurotransmitter-induced inositol phosphate formation in neurons in primary culture. J. Neurochem. 50, 1425-1433.