Direct cloning of cell differential expression genes with full-length by a new strategy based on the multiple rounds of 'long distance' polymerase chain reaction and magnetic beads mediated subtraction

Journal of Biotechnology

Volumn 73, Issue 1, 1999, Pages 35-41

  Zhao, Zhongliang ^a   Huang, Xin ^a   Li, Nan ^a   Zhu, Xuejun ^a   Chen, Sumin ^b   Cao, Xuetao ^a  

^a SECOND MILITARY MEDICAL UNIVERSITY   (China)

^b FOURTH MILITARY MEDICAL UNIVERSITY   (China)

Author keywords

Dendritic cell; Polymerase chain reaction; Subtractive cloning

Indexed keywords

CELLS; CLONING; COLUMN CHROMATOGRAPHY; DNA; ENZYME KINETICS;

DENDRITIC CELL; POLYMERASE CHAIN REACTION;

GENES;

COMPLEMENTARY DNA; MESSENGER RNA;

ARTICLE; CELL LINE; COLUMN CHROMATOGRAPHY; DOT HYBRIDIZATION; HELA CELL; HUMAN; HUMAN CELL; MAGNETIC FIELD; MOLECULAR CLONING; POLYMERASE CHAIN REACTION; PRIORITY JOURNAL; TECHNIQUE;

CLONING, MOLECULAR; DENDRITIC CELLS; HELA CELLS; HUMANS; POLYMERASE CHAIN REACTION;

EID: 0344348877     PISSN: 01681656     EISSN: None     Source Type: Journal    
DOI: 10.1016/S0168-1656(99)00006-1     Document Type: Article

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