Non-invasive diagnosis of Pneumocystis carinii pneumonia by PCR on oral washes

Lancet

Volumn 350, Issue 9088, 1997, Pages 1363-

  Helweg Larsen, Jannik ^a   Jensen, Jørgen Skov ^a   Lundgren, Bettina ^a  

^a NONE

Author keywords

[No Author keywords available]

Indexed keywords

BACTERIAL DNA;

ARTICLE; CLINICAL ARTICLE; CLINICAL TRIAL; CONTROLLED CLINICAL TRIAL; CONTROLLED STUDY; DNA DETERMINATION; GEL ELECTROPHORESIS; GENE AMPLIFICATION; HUMAN; LUNG LAVAGE; PNEUMOCYSTIS PNEUMONIA; POLYMERASE CHAIN REACTION; PRIORITY JOURNAL;

EID: 0344301896     PISSN: 01406736     EISSN: None     Source Type: Journal    
DOI: 10.1016/S0140-6736(97)24045-6     Document Type: Article

References (5)

1. Yale SH, Limper AH. Pneumocystis carinii pneumonia in patients without acquired immunodeficiency syndrome: associated illness and prior corticosteroid therapy. Mayo Clin Proc 1996; 71: 5-13.
2. Wakefield AE, Miller RF, Guiver LA, Hopkin JM. Oropharyngeal samples for detection of Pneumocystis carinii by DNA amplification. Q J Med 1993; 86: 401-06.
3. Walsh PS, Metzger DA, Higuchi R. Chelex 100 as a medium for simple extraction of DNA for PCR-based typing from forensic material. Biotechniques 1991; 10: 506-13.
4. Wakefield AE, Pixley FJ, Banerji S, et al. Detection of Pneumocystis carinii with DNA amplification. Lancet 1990; 336: 451-53.
5. Don RH, Cox PT, Wainwright BJ, Baker K, Mattick JS. 'Touchdown' PCR to circumvent spurious priming during gene amplification. Nucleic Acids Res 1991; 19: 14.