Rapid isolation of single microbial cells from mixed natural and laboratory populations with the aid of a micromanipulator

Systematic and Applied Microbiology

Volumn 22, Issue 2, 1999, Pages 249-257

  Fröhlich, Jürgen ^a   König, Helmut ^a  

^a JOHANNES GUTENBERG UNIVERSITY   (Germany)

Author keywords

Archaea; Archaezoa; Bacteria; Isolation; Koruga bonita; Methanobrevibacter; Microbial cells; Micromanipulator; Mycoplasma; Single cells

Indexed keywords

RIBOSOME DNA;

ARCHAEBACTERIUM; ARTICLE; BACTERIAL GROWTH; BACTERIUM CULTURE; BACTERIUM ISOLATION; CELL CLONE; CELL NUCLEUS; FLORA; INSECT; ISOLATION PROCEDURE; MICROMANIPULATOR; MYCOPLASMA; NONHUMAN; POLYMERASE CHAIN REACTION; PRIORITY JOURNAL; PROTOZOON;

ARCHAEA; ARCHEZOA; BACTERIA (MICROORGANISMS); EUKARYOTA; INSECTA; ISOPTERA; KORUGA BONITA; METHANOBREVIBACTER; MYCOPLASMA; PROKARYOTA; PROTOZOA;

EID: 0344299237     PISSN: 07232020     EISSN: None     Source Type: Journal    
DOI: 10.1016/S0723-2020(99)80072-1     Document Type: Article

References (34)

1. BAKOSS, P.: Cloning of leptospires by micromanipulator. Bull. World Health Organ. 43, 599-601 (1970)
2. BERCHTOLD, M., KÖNIG, H.: The phylogenetic position of the two uncultivated trichomonads Pentatrichomonoides scroa Kirby and Metadevescovina extranea Kirby from the hindgut of the termite Mastotermes darwiniensis Froggatt. System. Appl. Microbiol. 18, 567-573 (1995)
3. BERCHTOLD, M., KÖNIG, H.: Phylogenetic analysis and in situ identification of uncultivated spirochetes from the hindgut of the termite Mastotermes darwiniensis. System. Appl. Microbiol. 19, 66-73 (1996)
4. BOWYER, J. W., SKERMAN, V. B. D.: Production of axenic cultures of soil-borne and endophytic blue-green algae. J. Gen. Microbiol. 54, 299-306 (1968)
5. BRADFORD, D., HUGENHOLTZ, P., SEVIOUR, E. M., CUNNINGHAM, M. A., STRATTON, H., SEVIOUR, R. J., BLACKALL, L. L.: 16S rRNA analysis of isolates obtained from gram-negative, filamentous bacteria micromanipulated from activated sludge. System. Appl. Microbiol. 19, 334-343 (1996)
6. BROSIUS, J., DULL, T., SLEETER, D. D., NOLLER, H .R: Gene organization and primary structure of a ribosomal RNA operon from Escherichia coli. Proc. Natl. Acad. Sci. USA 75, 4801-4805 (1981)
7. BRUGEROLLE, G., TAYLOR, F., J., R.: Taxonomy, cytology and evolution of the Mastigophora, pp. 14-28. In: HUTNER, S. H. (ed.), Proceedings of the Vth International Congress of Protozoology, New York, Pace University, 1977
8. BRUGEROLLE, G., BREUNIG, A., KÖNIG, H.: Ultrastructural study of Pentatrichotnonoides sp. a trichomonad flagellate from Mastotermes darwiniensis. Eur. J. Protistol, 30, 372-378 (1994)
9. CLEVELAND, L. R.: General features and reproduction in Mixotricha. Arch. Protistenk. 109, 26-36 (1966a)
10. CLEVELAND, L. R.: General features and reproduction in Koruga bonita, gen. et sp. nov. Arch. Protistenk. 109, 18-23 (1966b)
11. CLEVELAND, L. R.: General features of the flagellate and amoeboid stages of Deltotrichonympha operculata and D. nana, sp. nov. Arch. Protistenk. 109, 1-7 (1966c)
12. FELSENSTEIN, J.: Confidence limits on phylogenies: an approach using the bootstrap. Evolution 39, 783-791 (1985)
13. FELSENSTEIN, J.: Phylip 3.5. Department of genetics SK-50. University of Washington, Seattle, Washington 1993
14. FITCH, W.M., MARGOLIASH, E.: Construction of phylogenetic trees. Science 155, 279-284 (1967)
15. FRÖHLICH, J., KAHLE, D., KÖNIG, H.: Isolation of single bacteria from mixed populations with the aid of a micromanipulator. Biospectrum (special volume): 110 (1998a)
16. FRÖHLICH, J., KÖNIG, H., KAHLE, D.: Isolation of microorganisms. BioNews (Eppendorf) 10, 4 (1998b)
17. FRÖHLICH, J., KÖNIG, H.: Ethidium bromide: a fast fluorescent staining procedure for the detection of symbiotic partnership of flagellates and prokaryotes. J. Microbiol. Meth. 35, 121-127 (1999)
18. GERHARDT, P., MURRAY, R.G.E., COSTILOW, E.W., WOOD, W.A., KRIEG, N.R. (eds.): Manual of Methods for General Bacteriology. American Society for Microbiology. Washington 1994
19. HUBER, R., BURGGRAF, S., MAYER, T., BARNS, S. M., ROSSNAGEL, P., STETTER, K. O.: Isolation of a hyperthermophilic archaeum predicted by in situ RNA analysis. Nature 376, 57-58 (1995)
20. JUKKS, T. H., CANTOR, C. R.: Evolution of protein molecules, pp. 21-132. In: Mammalian Protein Metabolism (H. N. MUNRO, ed.). New York, Academic Press, 1969
21. KRÄMER, J.: Lebensmittel-Mikrobiologie. Ulmer, Stuttgart 1997
22. KEELING, P.J., POULSON, N. and MCFADDEN, G.I.: Phylogenetic diversity of parabasalian symbionts from termites, including the phylogenetic place of Trichonympha and Pseudotrypanosoma. J. Eukaryot. Microbiol. 45, 643-650 (1998)
23. LEADBETTER, J.R., BREZNAK, J. A.: Physiological ecology of Methanobrevibacter cuticularis sp. nov. and Methanobrevibacter curvatus sp. nov., isolated from the hindgut of the termite Reticulitermes flavipes. Appl. Environ. Microbiol. 62, 3620-3631 (1996)
24. MAIDAK, B. L., COLE, J. R., PARKER, C. T., GARRITY, G. M., LARSEN, N., LI, B., LILBURN, T. G., MCCAUGHEY, M.J., OLSEN, G. J., OVERBEEK, R., PRAMANIK, S., SCHMIDT, T. M., TIEDJE, J. M., WOESE, C. R.: A new version of the RDP (Ribosomal Database Project). Nucleic Acids Res. 27, 171-173 (1999)
25. MEDLIN, L., ELWOOD, H. J., STICKEL, S., SOGIN, M.L.: The characterization of enzymatically amplified eukaryotic 16S-like rRNA-coding regions. Gene 71, 491-499 (1988)
26. MILLER, T. L., WOLIN, M. J.: Methanogens in human and animal intestinal tracts. System. Appl. Microbiol. 7, 223-229 (1986)
27. MUYZER, G., DE WAAL, E. C., UITTERLINDEN A.C.: Profiling of complex microbial populations by denaturing gradient gel electrophoresis analysis of polymerase chain reaction-amplified genes encoding for 16S rRNA. Appl. Environ. Microbiol 59, 695-700 (1993)
28. NEWTON, C. R., GRAHAM, A.: PCR. Spectrum Verlag, Heidelberg 1997
29. OHKUMA, M., KUDO, T.: Phylogenetic analysis of the symbiotic intestinal microflora of the termite Cryptotermes domesticus. FEMS Microbiol. Lett. 164, 389-395 (1998)
30. OHKUMA, M., OHTOKO, K., GRUNAU, C., MORIYA, S., KUDO, T.: Phylogenetic identification of the symbiotic hypermastigote Trichonympha agilis in the hindgut of the termite Reticulitermes speratus based on small-subunit RNA sequence. J. Eukaryot. Microbiol. 45, 439-444 (1998)
31. PETTERSSON, B., UHLEN, M., JOHANSSON, K. E.: Phylogeny of some mycoplasmas from ruminants based on 16S rRNA sequences and definition of a new cluster within the hominis group. Int. J. System. Bacteriol. 46, 1093-1098 (1996)
32. SAITOU, N., NEI, M.: The neighbor joining method: a new method for reconstructing phylogenetic trees. Mol. Biol. Evol. 4, 406-425 (1987)
33. SHERMAN, F.: Micromanipulator for yeast genetic studies. Appl. Microbiol. 26, 829 (1973)
34. SKERMAN, V. B. D.: A new type of micromanipulator and microforge. J. Gen. Microbiol. 54, 287-297 (1968)