Single-spore isolation using a hand-made glass needle

Fungal Diversity

Volumn 2, Issue , 1999, Pages 47-63

  Goh, Teik Khiang ^a  

^a UNIVERSITY OF HONG KONG   (Hong Kong)

Author keywords

Fungi; Microbiological techniques; Pure cultures; Skills; Spores; Tools

Indexed keywords

EID: 0344274593     PISSN: 15602745     EISSN: None     Source Type: Journal    
DOI: None     Document Type: Article

References (13)

1. El-Badry, H.M. (1963). Monographien aus dem Gebiete der qualitativen Mikroanalyse. Band 3. Micromanipulators and micromanipulation. Springer-Verlag, Wien.
2. Fuller, M.S. and Clay, R.P. (1993). Observations of Gonapodya in pure culture: growth, development and cell wall characterization. Mycologia 85: 38-45.
3. Goh, T.K. and Hanlin, R.T. (1997). Nuclear divisions in the ascus and ascospores of Melanospora zamiae. Mycological Research 101: 1511-1514.
4. Goh, T.K. and Hanlin, R.T. (1998). Ultrastructural observation of ascomal development in Melanospora zamiae, Mycologia 90: 655-666.
5. Goh, T.K., Hyde, K.D. and Hanlin, R.T. (1998a). Spore germination, hyphal morphology, homothallism, and conidial state in Melanospora zamiae. Fungal Science 13: 1-9.
6. Goh, T.K., Hyde, K.D. and Lee, D.K.L. (1998b). Generic distinction in the Helminthosporium-complex based on restriction analysis of the nuclear ribosomal RNA gene. Fungal Diversity 1: 85-107.
7. Hansen, H.N. (1926). A simple method of obtaining single-spore cultures. Science 64: 384.
8. Hildebrand, E.M. (1938). Techniques for the isolation of single microorgansims. Botanical Review 4: 627-664.
9. Hsieh, W.H. and Goh, T.K. (1990). Cercospora and similar fungi from Taiwan. Maw Chang Book Company, Taiwan.
10. Kirsop, B.E. and Doyle, A. (1991). Maintenance of microorganisms and cultured cells, a manual of laboratory methods. 2nd edn. Academic Press, London.
11. Matsushima, T. (1975). Icones Microfungorum a Matsishima Lectorum. Matsushima, Kobe.
12. Sivichai, S., Goh, T.K., Hyde, K.D. and Hywel-Jones, N.L. (1998). The genus Brachydesmiella from submerged wood in the tropics, including a new species and a new combination. Mycoscience : (In press).
13. Smith, G. (1969). An Introduction to Industrial Mycology. 6th edn. Edward Arnold Ltd., London.