Analysis of bacteria in aquatic environments using sedimentation field-flow fractionation: (II) physical characterization of cells

Water Research

Volumn 32, Issue 5, 1998, Pages 1508-1514

  Sharma, Reshmi V ^a   Edwards, Rick T ^a   Beckett, Ronald ^a  

^a MONASH UNIVERSITY   (Australia)

Author keywords

Aquatic bacteria; Cell density; Cell size distributions; Sedimentation field flow fractionation

Indexed keywords

BACTERIA; CELLS; FRACTIONATION; SEDIMENTATION;

AQUATIC BACTERIA; CELL DENSITY; CELL SIZE DISTRIBUTION; SEDIMENTATION FIELD FLOW FRACTIONATION;

WATER BACTERIOLOGY;

ANALYTICAL TECHNIQUE; AQUATIC ENVIRONMENT; BACTERIA; CELL; SPECIFIC GRAVITY;

ARTICLE; BACTERIUM ISOLATION; CELL COUNT; CELL DENSITY; CELL SIZE; FRACTIONATION; NATURAL POPULATION; PRIORITY JOURNAL; RELATIVE DENSITY; WATER ANALYSIS;

BACTERIA (MICROORGANISMS);

EID: 0344269331     PISSN: 00431354     EISSN: None     Source Type: Journal    
DOI: 10.1016/S0043-1354(97)00398-9     Document Type: Article

References (21)

1. Beckett R. and Hart B. T. (1993) In Environmental Particles (Edited by Buffle J.), vol. 2, pp. 165-205. Lewis Publishers, Boca Raton, FL.
2. Bolter M., Moller R., Dzomla W. Determination of bacterial biovolume with epifluorescence microscopy: Comparison of size distributions from image analysis and size classifications. Micron. 24:1993;31-40.
3. Button D. K., Schut F., Quang P., Martin R., Robertson B. R. Viability and isolation of marine bacteria by dilution culture: Theory, procedures, and initial results. Appl. Environ. Microbiol. 59:1993;881-891.
4. Christensen H., Bakken L. R., Olsen R. A. Soil bacterial DNA and biovolume profiles measured by flow cytometry. FEMS Microbiol. Ecol. 102:1993;129-140.
5. Edwards R. T. Sestonic bacteria as a food source for filtering invertebrates in two south eastern blackwater rivers. Limnol. Oceanogr. 32:1987;221-234.
6. Ferguson R. L., Rublee P. Contribution of bacteria to standing crop of coastal plankton. Limnol. Oceanogr. 21:1976;141-145.
7. Fuhrman J. A. Influence of method on the apparent size distribution of bacterioplankton cells: Epifluorescence microscopy compared to scanning electron microscopy. Mar. Ecol. Prog. Ser. 5:1981;103-106.
8. Fuhrman J. A., Azam F. Thymidine incorporation as a measure of heterotrophic bacterioplankton production in marine surface waters: Evaluation and field results. Mar. Biol. 66:1982;109-120.
9. Giddings J. C. Field-flow fractionation: Analysis of macromolecular, colloidal and particulate materials. Sci. 260:1993;1456-1465.
10. Jepras R. I., Carter J., Pearson S. C., Paul F. E., Wilkinson M. J. Development of a robust flow cytometric assay for determining numbers of viable bacteria. Appl. Environ. Microbiol. 61:1995;2696-2701.
11. Kogure K., Koike I. Particle counter determination of bacterial biomass in sea water. Appl. Environ. Microbiol. 53:1987;274-277.
12. Monger B. C., Landry M. R. Flow cytometric analysis of marine bacteria with Hoechst 33342. Appl. Environ. Microbiol. 59:1993;905-911.
13. Morita R. Y. Starvation-survival of heterotrophs in the marine environment. Adv. Microbial Ecol. 6:1982;171-198.
14. Novitsky J. A., Morita R. Y. Morphological characterisation of small cells resulting from nutrient starvation of a psychrophilic marine vibrio. Appl. Environ. Microbiol. 32:1977;617-662.
15. Porter J., Edwards C., Morgan J. A. W., Pickup R. W. Rapid, automated separation of specific bacteria from lake water and sewage by flow cytometry and cell sorting. Appl. Environ. Microbiol. 59:1993;3327-3333.
16. Porter J., Diaper J., Edwards C., Pickup R. Direct measurements of natural planktonic bacterial community viability by flow cytometry. Appl. Environ. Microbiol. 61:1995;2783-2786.
17. Ross J., Boon P. I., Sharma R., Beckett R. Variations in the fluorescence intensity of intact DAPI-stained bacteria and their implications for rapid bacterial quantification. Lett. Appl. Microbiol. 22:1996;283-287.
18. Sharma R. (1994) Physical characterisation, quantification and biomass determination of bacteria using sedimentation field-flow fractionation. Ph.D. Thesis, Monash University, Melbourne, Australia.
19. Sharma R. V., Edwards R. T., Beckett R. Physical characterisation and quantification of bacteria by sedimentation field-flow fractionation. Appl. Environ. Microbiol. 59:1993;1864-1875.
20. Sharma R. V., Edwards R. T. and Beckett R. (1998) Analysis of bacteria in aquatic environments using sedimentation field-flow fractionation: (1) biomass determination. Wat. Res., 32, 1497 - 1507.
21. Williams P. S., Giddings J. C. Power Programmed Field-Flow Fractionation: A new program form for improved uniformity of fractionating power. Anal. Chem. 59:1987;2038.