A simple method for detecting up to five immunofluorescent parameters together with DNA staining for cell cycle or viability on a benchtop flow cytometer

Journal of Immunological Methods

Volumn 275, Issue 1-2, 2003, Pages 113-121

  Gordon, Kristie M ^a   Duckett, Larry ^b   Daul, Bruce ^b   Petrie, Howard T ^a,c,d  

^a MEMORIAL SLOAN KETTERING CANCER CENTER   (United States)

^b BD BIOSCIENCES   (United States)

^c Weill Cornell Graduate School of Biomedical Sciences   (United States)

^d Box 341 ^*  (United States)

Author keywords

Cell cycle analysis; DNA staining; Flow cytometry; Immunofluorescence

Indexed keywords

DNA;

ACCURACY; ANALYTIC METHOD; ANIMAL CELL; ARTICLE; BIOLOGICAL MODEL; CELL CYCLE; CELL POPULATION; CELL VIABILITY; CHEMICAL MODIFICATION; CONTROLLED STUDY; DATA ANALYSIS; DNA CONTENT; FLOW CYTOMETRY; IMMUNOFLUORESCENCE; IMMUNOPHENOTYPING; MEASUREMENT; MOUSE; NONHUMAN; PARAMETER; PRIORITY JOURNAL; STAINING; ULTRAVIOLET RADIATION;

EID: 0344177194     PISSN: 00221759     EISSN: None     Source Type: Journal    
DOI: 10.1016/S0022-1759(03)00009-7     Document Type: Article

References (13)

1. Crissman H.A., Steinkamp J.A. Rapid, simultaneous measurement of DNA, protein, and cell volume in single cells from large mammalian cell populations. J. Cell Biol. 59:1973;766.
2. Crissman H.A., Tobey R.A. Cell-cycle analysis in 20 minutes. Science. 184:1974;1297.
3. Gill J.E., Jotz M.M., Young S.G., Modest E.J., Sengupta S.K. 7-Amino-actinomycin D as a cytochemical probe: I. Spectral properties. J. Histochem. Cytochem. 23:1975;793.
4. Godfrey D.I., Kennedy J., Mombaerts P., Tonegawa S., Zlotnik A. Onset of TCR-beta gene rearrangement and role of TCR-beta expression during CD3-CD4-CD8- thymocyte differentiation. J. Immunol. 152:1994;4783.
5. Hirons G.T., Fawcett J.J., Crissman H.A. TOTO and YOYO: new very bright fluorochromes for DNA content analyses by flow cytometry. Cytometry. 15:1994;129.
6. Hoffman E.S., Passoni L., Crompton T., Leu T.M., Schatz D.G., Koff A., Owen M.J., Hayday A.C. Productive T-cell receptor beta-chain gene rearrangement: coincident regulation of cell cycle and clonality during development in vivo. Genes Dev. 10:1996;948.
7. Hopwood D. Cell and tissue fixation, 1972-1982. Histochem. J. 17:1985;389.
8. Mombaerts P., Iacomini J., Johnson R.S., Herrup K., Tonegawa S., Papaioannou V.E. RAG-1-deficient mice have no mature B and T lymphocytes. Cell. 68:1992;869.
9. Penit C., Lucas B., Vasseur F. Cell expansion and growth arrest phases during the transition from precursor (CD4-8-) to immature (CD4+8+) thymocytes in normal and genetically modified mice. J. Immunol. 154:1995;5103.
10. Prockop S., Petrie H.T. Cell migration and the anatomic control of thymocyte precursor differentiation. Semin. Immunol. 12:2000;435.
11. Rabinovitch P.S., Torres R.M., Engel D. Simultaneous cell cycle analysis and two-color surface immunofluorescence using 7-amino-actinomycin D and single laser excitation: applications to study of cell activation and the cell cycle of murine Ly-1 B cells. J. Immunol. 136:1986;2769.
12. Shapiro H.M., Stephens S. Flow cytometry of DNA content using oxazine 750 or related laser dyes with 633 nm excitation. Cytometry. 7:1986;107.
13. Zelenin A.V., Poletaev A.I., Stepanova N.G., Barsky V.E., Kolesnikov V.A., Nikitin S.M., Zhuze A.L., Gnutchev N.V. 7-Amino-actinomycin D as a specific fluorophore for DNA content analysis by laser flow cytometry. Cytometry. 5:1984;348.