Transformation of Trichoderma reesei with a constitutively expressed heterologous fungal chitinase gene

Enzyme and Microbial Technology

Volumn 24, Issue 7, 1999, Pages 419-424

  Deane, Eddie E ^a,b   Whipps, John M ^c   Lynch, James M ^c   Peberdy, John F ^a,b  

^a UNIVERSITY OF NOTTINGHAM   (United Kingdom)

^b UNIVERSITY OF WARWICK   (United Kingdom)

^c UNIVERSITY OF SURREY   (United Kingdom)

Author keywords

Chitinase; Constitutive expression; Transformation; Trichoderma reesei

Indexed keywords

BIOCONVERSION; CATALYST ACTIVITY; ENZYMES; GENES; MOLECULAR STRUCTURE;

CHITINASE;

FUNGI;

CHITINASE;

ARTICLE; CONTROLLED STUDY; ENZYME ACTIVITY; GEL FILTRATION CHROMATOGRAPHY; GENE EXPRESSION; MUTANT; NONHUMAN; TRICHODERMA REESEI;

APHANOCLADIUM ALBUM; ASPERGILLUS NIDULANS; TRICHODERMA REESEI;

EID: 0344141460     PISSN: 01410229     EISSN: None     Source Type: Journal    
DOI: 10.1016/S0141-0229(98)00155-0     Document Type: Article

References (26)

1. Ridout, C. J., Coley-Smith, J. R., and Lynch, J. M. Enzyme activity and electrophoretic profile of extracellular protein induced in Trichoderma spp. by cell walls of Rhizoctania solani. Enzyme Microb. Technol. 1986, 10, 180-187.
2. Deane, E. E., Whipps, J. M., Lynch, J. M., and Peberdy, J. F. The purification and characterization of a Trichoderma harzianum exochitinase. Biochim. Biophys. Acta 1998, 1383, 101-110.
3. Valadares-Inglis, M. C., Inglis, P. W., and Peberdy, J. F. Sequence analysis of the catalytic domain of a Metarhizium anisopliae chitinase. Braz. J. Genet. 1997, 20, 161-164.
4. Teeri, T., Jones, A., Kraulis, P., Rouvinen, J., Penttilä, M., Harkki, A., Nevalainen, H., Vanhanen, S., Saloheimo, M., and Knowles, J. K. C. Engineering Trichoderma and its cellulases. In: Trichoderma reesei Cellulases, Biochemistry, Genetics, Physiology and Application (Kubicek, C. P., Eveleigh, D. E., Esterbauer, H., Steiner W., and Kubicek-Pranz, E. M., Eds.). The Royal Society of Chemistry, Cambridge, 1990, 156-167.
5. Harman, G. E., Hayes, C. K., Lorito, M., Broadway, R. M., DiPietro, A., Peterbauer, C., and Tronsmo, A. Chitinolytic enzymes of Trichoderma harzianum: purification of chitobiosidase and endochitinase. Phytopathology 1993, 83, 313-318.
6. Lorito, M., Harman, G. E., Hayes, C. K., Broadway, R. M., Tronsmo, A., Woo, S. L., and Di Pietro, A. Chitinolytic enzymes produced by Trichoderma harzianum: antifungal activity of purified endochitinase and chitobiosidase. Phytopathology 1993, 83, 302-307.
7. Carsolio, C., Gutiérrez, A., Jiménez, B., Van Montagu, M., and Herrera-Estrella, A. Characterisation of ech-42, a Trichoderma harzianum endochitinase gene expressed during mycoparasitism. Proc. Natl. Acad. Sci. 1994, 91, 10903-10907.
8. van Gorcom, R. F. M., Punt, P. J., and van den Hondel, C. A. M. J. J. Heterologous gene expression in Aspergillus. In: The Genus Aspergillus, from Taxonomy and Genetics to Industrial Application (Powell, K. A., Renwick, A., and Peberdy, J. F., Eds.). Plenum Press, New York, 1994, 241-251.
9. Christensen, T. Application of Aspergillus oryzae as a host for production of industrial enzymes. In: The Genus Aspergillus, from Taxonomy and Genetics to Industrial application (Powell, K. A., Renwick, A., and Peberdy, J. F., Eds.). Plenum Press, New York, 1994, 251-261.
10. Harkki, A., Usuitalo, M., Bailey, M., Penttilä, M., and Knowles, J. K. C. A novel fungal expression system: secretion of active calf chymosin from the filamentous fungus Trichoderma reesei. Bio/Technology 1989, 7, 596-603.
11. Margolles-Clark, E., Hayes, C. K., Harman, G. E., and Penttilä, M. Improved production of Trichoderma harzianum endochitinase by expression in Trichoderma reesei. Appl. Environ. Microbiol. 1996, 62, 2145-2151.
12. Berges, T. and Barreau, C. Isolation of uridine auxotrophs from Trichoderma reesei and efficient transformation with the cloned ura3 and ura5 genes. Curr. Genet. 1991, 19, 359-365.
13. Punt, P. J., Zegers, N. D., Busscher, M., Pouwels, P. H., and van den Hondel, C. A. M. J. J. Intracellular and extracellular production of proteins in Aspergillus under the control of expression signals of the highly expressed A. nidulans gpdI gene. J. Biotechnol. 1991, 17, 19-34.
14. Hamlyn, P. F., Bradshaw, R. E., Mellon, F. M., Santiago, C. M., Wilson, J. M., and Peberdy, J. F. Efficient protoplast isolation from fungi using commercial enzymes. Enzyme Microb. Technol. 1981, 3, 321-325.
15. Wirth, S. J. and Wolf, G. A. Dye-labelled substrates for the assay and detection of chitinase and lysozyme activity. J. Microbiol. Meth. 1990, 12, 197-205.
16. Raeder, U. and Broda, P. Rapid preparation of DNA from filamentous fungi. Lett. Appl. Microbiol. 1985, 1, 17-20.
17. Sambrook, J., Firtsch, E. F., and Maniatis T. Molecular Cloning a Laboratory Manual, 2nd Ed. Cold Spring Harbor, New York, 1989.
18. Reissig, J. L., Strominger, J. L., and Leloir, L. F. A modified colorimetric method for the estimation of N-acetylamino sugars. J. Biol. Chem. 1955, 217, 959-966.
19. Bradford, M. M. A rapid and sensitive method for quantitation of microgram quantities of protein utilizing the principle of protein dye binding. Anal. Biochem. 1976, 72, 504-511.
20. Sokolovsky, V., Kaldenhoff, R., Ricci, M., and Russo, V. E. A. Fast and reliable mini-prep RNA extraction from Neurospora crassa. Neurospora Newslett. 1990, 37, 41-43.
21. Gruber, F., Visser, J., Kubicek, C. P., and de Graaff, L. H. The development of a heterologous transformation system for the cellulolytic fungus Trichoderma reesei based on pyrG negative mutant. Curr. Genet. 1990, 18, 71-76.
22. Goldman, G. H., Van Montagu, M., and Herrera-Estrella, A. Transformation of Trichoderma harzianum by high voltage electric pulse. Curr. Genet. 1990, 17, 169-174.
23. Penttilä, M., Nevalainen, H., Ratto, M., Salminen, E., and Knowles, J. A versatile transformation system for the cellulolytic filamentous fungus Trichoderma reesei. Gene 1987, 61, 155-164.
24. Peberdy, J. F. Protein secretion in filamentous fungi-trying to understand a highly productive black box. TIBTECH. 1994, 12, 50-57.
25. Kunz, C., Sellam, O., and Bertheau, Y. Purification and characterization of a chitinase from the hyperparasitic rust fungus Aphanocladium album. Physiol. Mol. Plant Pathol. 1992, 40, 117-131.
26. Goldman, G. H., Hayes, C., and Harman, G. E. Molecular and cellular biology of biocontrol by Trichoderma sp. Trends Biotechnol. 1994, 12, 478-482.