Enhanced in vitro maturation of subcultivated fetal human hepatocytes in three dimensional culture using poly-L-lactic acid scaffolds in the presence of oncostatin M

International Journal of Artificial Organs

Volumn 26, Issue 10, 2003, Pages 943-951

  Hanada, Sanshiro ^a   Kayano, H ^b   Jiang, J ^a,c   Kojima, N ^d   Miyajima, A ^e   Sakoda, A ^a   Sakai, Y ^a  

^a INSTITUTE OF INDUSTRIAL SCIENCE   (Japan)

^b Tokyo College of Biotechnology   (Japan)

^c JILIN UNIVERSITY   (China)

^d KANAGAWA ACADEMY OF SCIENCE AND TECHNOLOGY   (Japan)

^e UNIVERSITY OF TOKYO   (Japan)

Author keywords

Albumin; Cytochrome P450 1A1 2; Fetal human hepatocytes; Maturation; Oncostatin M; Poly L lactic acid; Three dimensional culture; fetoprotein

Indexed keywords

CELLS; CYTOLOGY; LACTIC ACID;

ALBUMIN; CYTOCHROME P450 1A1/2; CYTOCHROMES P450; FETAL HUMAN HEPATOCYTE; FETOPROTEIN; HUMAN HEPATOCYTES; MATURATION; ONCOSTATIN M; POLY-L-LACTIC ACIDS; THREE-DIMENSIONAL CULTURE; Α-FETOPROTEIN;

RESTORATION;

ALBUMIN; ALPHA FETOPROTEIN; CYTOCHROME P450 1A1; CYTOCHROME P450 1A2; ONCOSTATIN M; POLYLACTIC ACID; RECOMBINANT SCATTER FACTOR;

ARTICLE; CELL FRACTIONATION; CELL MATURATION; CELL STRAIN HEPG2; CELL STRUCTURE; CELL VIABILITY; COMPARATIVE STUDY; CONTROLLED STUDY; CULTURE MEDIUM; CULTURE TECHNIQUE; DRUG EFFECT; FETUS; FETUS CELL; HUMAN; HUMAN CELL; IN VITRO STUDY; LIVER CELL; LIVER CELL CARCINOMA; LIVER FUNCTION; MONOLAYER CULTURE; PROTEIN SYNTHESIS; STEM CELL; THREE DIMENTIONAL CULTURE; TIME; TISSUE ENGINEERING;

EID: 0344121842     PISSN: 03913988     EISSN: None     Source Type: Journal    
DOI: 10.1177/039139880302601012     Document Type: Article

References (20)

1. Cai H, Nakazawa F, Ramachandran V, Thompson M, Fischer RA, Strom S. Isolation of fetal hepatocytes for clinical transplantation. Cell Transplant 2002; 10: 538.
2. Yamashita Y, Shimada M, Tsujita B, Tanaka S, Ijima H, Nakazawa K, Sakiyama R, Fukuda J, Ueda T, Funatsu K, Sugimachi K. Polyurethane form/spheroids culture system using human hepatoblastoma cell line (HepG2) as a possible new hybrid artificial liver. Cell Transplant 2001; 10: 717-22.
3. Yoon J-H, Lee H V-S, Lee JS, Park JB, Kim CY. Development of a non-transformed human liver cell line with differentiated-hepatocyte and urea-synthetic functions: Applicable for bioartificial liver. Int J Artif Organs 1999; 18: 2127-36.
4. Kamiya A, Kinoshita T, Ito Y, Matsui T, Morikawa Y, Senba B, Nakashima K, Taga T, Yoshida K, Kishimoto T, Miyajima A. Fetal liver development requires a paracrine action of oncostatin M through the gp130 signal transducer. EMBO J 1999; 18: 2127-36
5. Kojima N, Kinoshita T, Kamiya A, Nakamura K, Nakashima K, Taga T, Miyajima A. Cell density-dependent regulation of hepatic development by a gp130-independent pathway. Biochem Biophys Res Commun 2000; 277: 152-8.
6. Sakai Y, Jiang J, Kojima N, Kinoshita T, Miyajima A. Enhanced in vitro maturation of fetal mouse liver cells with oncostatin M, nicotinamide and dimethylsulfoxide. Cell Transplant 2002; 11: 435-41.
7. Kinoshita T, Sekiguchi T, Xu MJ, Ito Y, Kamiya A, Tsuji K, Nakamura T. Hepatic differentiation induced by oncostatin M attenuates fetal liver hematopoiesis. Proc Natl Acad Sci USA 1999; 96: 7265-70.
8. Miyoshi H, Ehashi T, Ema H, Hsiang CH, Nakauchi H, Ohshima N. Long-term culture of fetal liver cells using a three-dimensional porous polymer substrate. ASAIO J 2000; 46: 397-402.
9. Jiang J, Kojima N, Kinoshita T, Miyajima A, Yan W, Sakai Y. Cultivation of fetal liver cells in a three-dimensional poly-L-lactic acid scaffold in the presence of oncostatin M. Cell Transplant 2002; 11: 403-6.
10. Tateno T, Yoshizato K. Growth and differentiation in culture of clonogenic hepatocytes that expresses both phenotypes of hepatocytes and biliary epithelial cells. Am J Pathol 1996; 149: 1593-605.
11. Macdonald DJ, Kelly AM. The rapid quantification of serum alfa-fetoprotein by two-site microenxyme immunoassay. Clin Chim Acta 1978; 87: 367-72.
12. Sakai Y, Naruse K, Nagashima I, Muto T, Suzuki M. A new bioartificial liver using porcine hepatocyte spheroids in high-cell-density suspension perfusion culture: In vivo performance in synthesized culture medium and 100% human plasma. Cell Transplant 1999; 8: 531-41.
13. Donato MT, Gomez-Lechon M, Castell JV. A microassay for measuring cytochrome P450IA1 and P450IIB1 activities in intact human and rat hepatocytes cultured on 96-well plates. Anal Biochem 1993; 213: 29-33.
14. Hammond DK, Strobel HW. Ethoxyresorufin O-deethylase activity in intact human cells. Toxicol In Vitro 1992; 6: 41-6.
15. Brunk CF, Jones KC, James TW. Assay for nanogram quantities of DNA in cellular homogenates. Anal Biochem 1979; 92: 497-500.
16. Evarts RP, Nagy P, Marsden E, Thorgeirsson SS. A precursor relationship exists between oval cells and hepatocytes in rat liver. Carcinogenesis 1987; 8: 1737-40.
17. Mitaka T, Sattler GL, Putot HC, Michizuki Y. Characteristics of small cell colonies developing in primary cultures of adult rat hepatocytes. Virchows Archiv B Cell Pathol 1992; 62: 329-35.
18. Mitaka T, Sato F, Mizoguchi T, Yokono T, Mochizuki Y. Reconstruction of hepatic organoid by rat small hepatocytes and hepatic nonparenchymal cells. Hepatology 1999; 29: 111-25.
19. Kamiya A, Kojima N, Kinoshita T, Sakai Y, Mijayima A. Maturation of fetal hepatocytes in vitro by extracellular matrices and oncostatin M: Induction of tryptophan oxygenase. Hepatology 2002; 35: 1351-9.
20. Lazar A, Peshwa MV, Wu FJ, Chi C-M, Cerra FB, Hu W-S. Formation of porcine hepatocytes spheroids for use in a bioartificial liver. Cell Transplant 1995; 4: 259-68.