Evaluation of spent medium recycle and nutrient feeding strategies for recombinant protein production in the insect cell-baculovirus process

Journal of Biotechnology

Volumn 66, Issue 2-3, 1998, Pages 109-116

  Wu, Jianyong ^a   Ruan, Qian ^a   Lam, H Y Peter ^b  

^a HONG KONG POLYTECHNIC UNIVERSITY   (Hong Kong)

^b HONG KONG UNIVERSITY OF SCIENCE AND TECHNOLOGY   (Hong Kong)

Author keywords

Galactosidase; Baculovirus; Insect cell; Nutrient feeding; Spent medium recycle

Indexed keywords

AMINES; BATCH CELL CULTURE; BIOTECHNOLOGY; ENZYMES; GLUCOSE; GROWTH KINETICS; VIRUSES;

GLUTAMINE; YEASTOLATE;

PROTEINS;

BETA GALACTOSIDASE; RECOMBINANT PROTEIN;

ARTICLE; BACULOVIRUS; BIOTECHNOLOGY; CELL DENSITY; CELL GROWTH; CELL LINE; CONTROLLED STUDY; INSECT; NONHUMAN; NUTRIENT; PRIORITY JOURNAL; PROTEIN EXPRESSION; PROTEIN SYNTHESIS; RECOMBINANT DNA TECHNOLOGY;

EID: 0344073998     PISSN: 01681656     EISSN: None     Source Type: Journal    
DOI: 10.1016/S0168-1656(98)00057-1     Document Type: Article

References (14)

1. Büntemeyer H., Wallerius C., Lehmann J. Optimal medium use for continuous high density perfusion processes. Cytotechnology. 9:1992;59-67.
2. Bédard C., Kamen A., Tom R.L., Massie B. Maximization of recombinant protein yield in the insect cell/baculovirus system by one-time addition of nutrients to high-density batch cultures. Cytotechnology. 15:1994;129-138.
3. Caron A.W., Archambault J., Massie B. High-level recombinant protein production in bioreactors using the baculovirus-insect cell expression system. Biotechnol. Bioeng. 36:1990;1133-1140.
4. Drews M., Paalme T., Vilu R. The growth and nutrient utilization of the insect cell line Spodoptera frugiperda Sf-9 in batch and continuous culture. J. Biotechnol. 40:1995;187-198.
5. Glassy M.C., Tharakan J.P., Chau P.C. Serum-free media in hybridoma culture and monoclonal antibody production. Biotechnol. Bioeng. 32:1988;1015-1028.
6. Kempken R., Büntemeyer H., Lehmann J. The medium cycle bioreactor (MCB): monoclonal antibody production in a new economic production system. Cytotechnology. 7:1991;63-74.
7. Lindsay D.A., Betenbaugh M.J. Quantification of cell culture factors affecting recombinant yields in baculovirus-infected insect cells. Biotechnol. Bioeng. 39:1992;614-618.
8. Luckow, V.A., 1992. Cloning and expression of heterologous genes in insect cells with baculovirus vectors. In: Prokop, A., Bajpai, R.K., Ho, C.S. (Eds.), Recombinant DNA Technology and Applications. McGraw-Hill, New York, pp. 97-152.
9. Miller, J.H., 1972. Experiments in Molecular Genetics. Cold Spring Harbour Laboratory Press, New York.
10. Miller L.K. Baculoviruses: high-level expression insect cells. Curr. Opin. Genet. Dev. 3:1993;97-101.
11. Nguyen B., Jarnagin K., Williams S., Chan H., Barnett J. Fed-batch culture of insect cells: a method to increase the yield of the recombinant human nerve growth factor (rhNGF) in the baculovirus expression system. J. Biotechnol. 31:1993;205-217.
12. O'Reilly, D.R., Miller, L.K., Luckow, V.A., 1992. Baculovirus Expression Vectors. A Laboratory Manual. W.H. Freeman, New York.
13. Reuveny S., Kim Y.J., Kemp C.W., Shiloach J. Production of recombinant proteins in high-density insect cell cultures. Biotechnol. Bioeng. 42:1993;235-239.
14. Wang M.Y., Kwong S., Bentley W.E. Effects of oxygen/glucose/glutamine feeding on insect cell baculovirus protein expression: a study on epoxide hydrolase production. Biotechnol. Prog. 9:1993;355-361.