An alternative purification protocol for producing hepatitis B virus X antigen on a preparative scale in Escherichia coli

Journal of Biotechnology

Volumn 56, Issue 2, 1997, Pages 81-88

  Marczinovits, Ilona ^a   Somogyi, Csilla ^b   Patthy, András ^c   Németh, Péter ^b   Molnár, János ^a  

^a UNIVERSITY OF SZEGED   (Hungary)

^b UNIVERSITY OF PÉCS   (Hungary)

^c SZENT ISTVÁN UNIVERSITY   (Hungary)

Author keywords

Escherichia coli; Hepatitis B virus; High yield; Recombinant; Refolding; X protein

Indexed keywords

AMMONIUM COMPOUNDS; ANTIGENS; CHEMICAL BONDS; COLIFORM BACTERIA; GENES; PH EFFECTS; PRECIPITATION (CHEMICAL); PROTEINS; PURIFICATION; THERMAL EFFECTS; VIRUSES;

CELL LYSATE; ESCHERICHIA COLI; GUANIDINE HYDROCHLORIDE; HEPATITIS B VIRUS;

CLONING;

ANTIGEN EXPRESSION; ANTIGEN PURIFICATION; ARTICLE; DIAGNOSTIC VALUE; ESCHERICHIA COLI; HEPATITIS B VIRUS; MOLECULAR CLONING; NONHUMAN; PRIORITY JOURNAL; PROTEIN FOLDING;

AMINO ACID SEQUENCE; ANTIBODIES, MONOCLONAL; BLOTTING, WESTERN; CHROMATOGRAPHY, GEL; CLONING, MOLECULAR; ELECTROPHORESIS, POLYACRYLAMIDE GEL; ESCHERICHIA COLI; FACTOR XA; GENE EXPRESSION; GUANIDINE; GUANIDINES; HEPATITIS B ANTIGENS; MOLECULAR SEQUENCE DATA; PEPTIDE FRAGMENTS; PLASMIDS; PROTEIN DENATURATION; PROTEIN FOLDING; RECOMBINANT FUSION PROTEINS; TRANS-ACTIVATORS;

ESCHERICHIA COLI; HEPATITIS B VIRUS;

EID: 0343924606     PISSN: 01681656     EISSN: None     Source Type: Journal    
DOI: 10.1016/S0168-1656(97)00080-1     Document Type: Article

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