An improved fractionation and fast screening method for the identification of new and selective neurotoxins

Neuroscience Research

Volumn 24, Issue 2, 1996, Pages 201-206

  Debont, Tom ^a   Daenens, Paul ^a   Tytgat, Jan ^a  

^a UNIVERSITY OF LEUVEN   (Belgium)

Author keywords

Channel; Gel filtration; Oocyte; Potassium; Toxin; Venom; Voltage clamp

Indexed keywords

CELL RECEPTOR; COMPLEMENTARY DNA; ION CHANNEL; NEUROPEPTIDE; NEUROTOXIN; POTASSIUM CHANNEL; VENOM;

ANIMAL CELL; ARTICLE; CONTROLLED STUDY; EXPRESSION VECTOR; FAST PROTEIN LIQUID CHROMATOGRAPHY; FRACTIONATION; GEL FILTRATION CHROMATOGRAPHY; ION EXCHANGE CHROMATOGRAPHY; NERVOUS SYSTEM; NONHUMAN; OOCYTE; PRIORITY JOURNAL; REVERSED PHASE LIQUID CHROMATOGRAPHY; TOXICITY TESTING; VOLTAGE CLAMP; XENOPUS LAEVIS;

EID: 0343897938     PISSN: 01680102     EISSN: None     Source Type: Journal    
DOI: 10.1016/0168-0102(95)00982-5     Document Type: Article

References (13)

1. Baumann, A., Grupe, A., Ackermann, A. and Pongs, O. (1988) Structure of the voltage dependent potassium channel is highly conserved from Drosophila to vertebrate central nervous system. EMBO J., 7: 2457-2463.
2. Benishin, C., Sorensen, R., Brown, W., Kreuger, B. and Blaustein, M. (1988) Four polypeptide components of green mamba venom selectively block certain potassium channels in rat brain synaptosomes. Mol. Pharmacol., 34: 152-159.
3. Elkins, T., Ganetzky, B. and Wu, C.F. (1986) A Drosophila mutation that eliminates a calcium-dependent potassium current. Proc. Natl. Acad. Sci. USA, 83: 8415-8419.
4. + channels from Leiurus quinquestriatus var. hebraeus venom. Biochemistry, 33: 6834-6839.
5. Garcia-Calvo, M., Knaus, H-G., Garcia, M., Kaczorowski, G. and Kempner, E. (1994) Functional unit size of the charybdotoxin receptor in smooth muscle. Proc. Natl. Acad. Sci. USA, 91: 4718-4722.
6. Harvey, A.L. and Karlsson, E. (1980) Dendrotoxin from the venom of the green mamba, Dendroaspis angusticeps. Naunyn-Schmiedeberg's Arch. Pharmacol., 312: 1-6.
7. + channels depolarize human T lymphocytes: mechanism of the antiproliferative effect of charybdotoxin. Proc. Natl. Acad. Sci. USA, 89: 10094-10098.
8. + channel determined by construction of multimeric cDNAs. Neuron, 9: 861-871.
9. MacKinnon, R. and Miller, C. (1989) Mutant potassium channels with altered binding of charybdotoxin, a pore-blocking peptide inhibitor. Science, 245: 1382-1385.
10. + channels share common structural features. Neuron, 1: 997-1001.
11. +-channel protein identified by its binding properties for dendrotoxin I. Proc. Natl. Acad. Sci. USA, 85: 4919-4923.
12. Rees, B. and Bilwes, A. (1993) Three-dimensional structures of neurotoxins and cardiotoxins. Chem. Res. Toxicology, 6: 385-406.
13. Savel-Niemann, A. (1989) Tarantula (Eurypelma californicum) venom, a multi-component system. Biol. Chem. Hoppe-Seyler, 370: 485-498.