Molecular species analysis and quantification of the glycosylphosphatidylinositol intermediate glycolipid C from Trypanosoma brucei

Molecular and Biochemical Parasitology

Volumn 77, Issue 2, 1996, Pages 137-145

  Güther, Maria Lucia S ^a   Treumann, Achim ^a   Ferguson, Michael A J ^a  

^a UNIVERSITY OF DUNDEE   (United Kingdom)

Author keywords

Electrospray; Glycosylphosphatidylinositol; Trypanosome

Indexed keywords

GLYCOLIPID; GLYCOSYLPHOSPHATIDYLINOSITOL;

ANALYTIC METHOD; ARTICLE; CELL FREE SYSTEM; DEACYLATION; GLYCOSYLATION; LIPID METABOLISM; MOLECULAR BIOLOGY; NONHUMAN; PHOSPHOLIPID MEMBRANE; PRIORITY JOURNAL; QUANTITATIVE DIAGNOSIS; TRYPANOSOMA BRUCEI;

EID: 0343867216     PISSN: 01666851     EISSN: None     Source Type: Journal    
DOI: 10.1016/0166-6851(96)02585-6     Document Type: Article

References (20)

1. Krakow, J.L., Hereld, D., Bangs, J.D., Hart, G.W. and Englund, P.T. (1986) Identification of a glycolipid precursor of the Trypanosoma brucei variant surface glycoprotein. J. Biol. Chem. 261, 12147-12153.
2. Menon, A.K., Mayor, S., Ferguson, M.A.J., Duszenko, M. and Cross, G.A.M. (1988) A candidate glycophospholipid precursor for the membrane anchor of Trypanosoma brucei VSG. J. Biol. Chem. 263, 1970-1977.
3. Masterson, W.J., Doering, T.L., Hart, G.W. and Englund, P.T. (1989) A novel pathway for glycan assembly: biosynthesis of the glycosyl-phosphatidylinositol anchor of the trypanosome variant surface glycoprotein. Cell 56, 793-800.
4. Masterson, W.J., Raper, J., Doering, T.L., Hart, G.W. and Englund, P.T. (1990) Fatty acid remodeling: A novel reaction sequence in the biosynthesis of trypanosome glycosyl-phosphatidylinositol membrane anchors. Cell 62, 73-80.
5. Menon, A., Schwarz, R., Mayor, S. and Cross, G.A.M. (1990) Cell-free synthesis of glycosyl-phosphatidylinositol precursors for the glycolipid membrane anchor of Trypanosoma brucei variant surface glycoproteins. J. Biol. Chem. 265, 9033-9042.
6. Menon, A.K., Eppinger, M., Mayor, S. and Schwarz, R.T. (1993) Phosphatidylethanolamine is the donor of the terminal phosphoethanolamine group in trypanosome glycosylphosphatidylinositols. EMBO J. 12, 1907-1914.
7. Doering, T.L., Pessin, M.S., Hart, G.W., Raben, D.M. and Englund, P.T. (1994) The fatty acids in unremodelled trypanosome glycosyl-phosphatidylinositols. Biochem. J. 299, 741-746.
8. Güther, M.L.S., Masterson, W.J. and Ferguson, M.A.J. (1994) The effects of phenylmethylsulfonyl fluoride on inositol-acylation and fatty acid remodelling in African trypanosomes. J. Biol. Chem. 269, 18694-18701.
9. Güther, M.L.S. and Ferguson, M.A.J. (1995) The role of inositol acylation and inositol deacylation in GPI biosynthesis in Trypanosoma brucei. EMBO J. 14, 3080-3093.
10. Mayor, A., Menon, A.K. and Cross, G.A.M. (1990) Glycolipid precursors for the membrane anchor of Trypanosoma brucei variant surface glycoproteins. J. Biol. Chem. 265, 6174-6181.
11. Cross, G.A.M. (1975) Identification, purification and properties of clone-specific glycoprotein antigens constituting the surface coat of Trypanosoma brucei. Parasitology 71, 393-417.
12. Ferguson, M.A.J. (1992) Chemical and enzymic analysis of glycosyl phosphatidylinositol anchors. In: Lipid Modifications of Proteins: A Practical Approach (Hooper, N.M. and Turner, A.J., eds.), pp. 191-230. IRL Press, Oxford, UK.
13. Treumann, A., Lifely, M.R., Schneider, P. and Ferguson, M.A.J. (1995) Primary structure of CD52. J. Biol. Chem. 270, 6088-6099.
14. Doering, T.L., Pessin, M.S., Hoff, E.F., Hart, G.W., Raben, D.M. and Englund, P.T. (1993) Trypanosome metabolism of myristate, the fatty acid required for the variant surface glycoprotein membrane anchor. J. Biol. Chem. 268, 9215-9222.
15. Roberts, W.L., Santikarn, S., Reinhold, V.M. and Rosenberry, T.L. (1988) Structural characterization of the glycoinositol phospholipid membrane anchor of human erythrocyte acetylcholinesterase by fast atom bombardment mass spectrometry. J. Biol. Chem. 263, 18776-18784.
16. Sevlever, D., Humphrey, D. R. and Rosenberry, T. (1995) Compositional analysis of glucosaminyl (acyl)-phosphatidylinositol accumulated in HeLa S3 cells. Eur. J. Biochem. 233, 384-394.
17. Stevens, V.L. and Zhang, H. (1994) Coenzyme A dependence of glycosylphosphatidylinositol biosynthesis in a mammalian cell-free system. J. Biol. Chem. 269, 31397-31403.
18. Stevens, V.L. (1995) Biosynthesis of glycosylphosphatidylinositol membrane anchors. Biochem. J. 310, 361-370.
19. Field, M.C., Menon, A.K. and Cross, G.A.M. (1991) A glycosylphosphatidylinositol protein anchor from procyclic stage Trypanosoma brucei: lipid structure and biosynthesis. EMBO J. 10, 2731-2739.
20. Mayor, S., Menon, A.K., Cross, G.A.M., Ferguson, M.A.J., Dwek, R.A. and Rademacher, T.W. (1990) Glycolipid precursors for the membrane anchor of Trypanosoma brucei variant surface glycoproteins. J. Biol. Chem. 265, 6164-6173.