β-Carotene and inhibitors of iron absorption modify iron uptake by Caco-2 cells

Journal of Nutrition

Volumn 130, Issue 1, 2000, Pages 5-9

  García Casal, Maria Nieves ^a   Leets, Irene ^a   Layrisse, Miguel ^a  

^a INSTITUTO VENEZOLANO DE INVESTIGACIONES CIENTÍFICAS   (Venezuela)

Author keywords

Caco 2 cells; Iron uptake; Phytates; Polyphenols; Vitamin A; carotene

Indexed keywords

ASCORBIC ACID; BETA CAROTENE; FERROUS FUMARATE; PHYTATE; RETINOL; TANNIN;

ARTICLE; CELL STRAIN CACO 2; CONTROLLED STUDY; DRUG DETERMINATION; DRUG EFFECT; HUMAN; HUMAN CELL; IRON ABSORPTION; IRON METABOLISM; IRON TRANSPORT;

EID: 0343851553     PISSN: 00223166     EISSN: None     Source Type: Journal    
DOI: 10.1093/jn/130.1.5     Document Type: Article

References (33)

1. Alvarez-Hemandez, X., Nichols, G. S. & Glass, J. (1991) Caco-2 cell line: A system for studying iron transport across epithelial cell monolayers. Bioch. Biophys. Acta 1070: 205-208.
2. Artursson, P. (1990) Epithelial transport of drugs in cell culture. I: A model for studying the passive diffusion of drugs over intestinal absorptive (Caco-2) cells. J. Pharm. Sci. 79: 476-482.
3. Dix, C., Hassan, F., Obray, H., Shah, R. & Wilson, G. (1990) The transport of vitamin B12 through polarized monolayers of Caco-2 cells. Gastroenterology 98: 1272-1279.
4. During, A., Albaugh, G. & Smith, C. (1998) Characterization of β-carotene 15, 15′-dioxygenase activity in TC7 clone of human intestinal cell line Caco-2. Biochem. Biophys. Res. Comm. 249: 467-474.
5. Fogh, J., Fogh, J. M. & Orfeo, T. (1977) One hundred and twenty seven cultured human tumor cell lines producing tumors in nude mice. J Natl. Cancer Inst. 59: 221-226.
6. García-Casal, M., Layrisse, M., Solano, L., Barón, M. A., Arguello, F., Llovera, D., Ramirez, J., Leets, I. & Trapper, E. (1998) Vitamin A and β-carotene can improve nonheme iron absorption from rice, wheat and corn by humans. J. Nutr. 128: 646-650.
7. García, M., Flowers, C. & Cook, J. (1996) The Caco-2 cell culture system can be used as a model to study food iron availability. J. Nutr. 126: 251-258.
8. Halleux, C. & Scheider, Y. (1989) Development of a model in vitro to study iron absorption by human intestinal epithelial cells. Arch. Int. Physiol. Biochim. 97(2): B34.
9. Halleux, C. & Scheider Y. (1994) Iron absorption by Caco-2 cells cultivated in serum-free medium as an in vitro model of the human intestinal epithelial barrier. J. Cel. Physiol. 158: 17-28.
10. Han, O., Failla, M., Hill, D., Morris, E. & Smith, C. (1994) Inositol phosphates inhibit uptake and transport of iron and zinc by a human intestinal cell line. J. Nutr. 124: 580-587.
11. Han, O., Failla, M., Hill, D., Morris, E. & Smith, C. (1995a) Reduction of Fe(III) is required for uptake of nonheme iron by Caco-2 cells. J. Nutr. 125: 1291-1299.
12. Han, O., Failla, M., Hill, D., Morris, E. & Smith, C. (1995b) Ascorbate offsets the inhibitory effect of inositol phosphates on iron uptake and transport by Caco-2 cells. Proc. Soc. Exper. Biol. Med. 210: 50-56.
13. Hauri, H., Sterchi, E., Bienz, D. & Fransen, J. (1985) Expression and intracellular transport of microvillous membranes hydrolases in human intestinal epithelial cells. J. Cell Biol. 101: 838-851.
14. Hidalgo, I. & Borchardt, R. (1990) Transport of large neutral amino acids (phenylalanine) in a human intestinal epithelial cell line: Caco-2. Bioch. Biophys. Acta 1028: 25-30.
15. Hidalgo, I., Raub, T. & Borchardt, R. (1989) Characterization of the human colon carcinoma cell line (Caco-2) as a model system for intestinal epithelial permeability. Gastroenterology 96: 736-749.
16. Hughson, E. & Hopkins, C. (1990) Endocytic pathways in polarized Caco-2 cells: identification of an endosomal compartment accessible for both apical and basolateral surfaces. J. Cell Biol. 110: 337-348.
17. Koessler, K., Mauer, S. & Loughlin, R. (1926) The relation of anemia, primary and secondary, to vitamin A deficiency. J. Am. Med. Assoc. 87: 476-482.
18. Layrisse, M., Chavez, J. F., Méndez-Castellano, H., Bosch, V., Tropper, E., Bastardo, B. & González, E. (1996) Early response to the impact of iron fortification in the Venezuelan population. Am. J. Clin. Nutr. 64: 903-907.
19. Layrisse, M., García-Casal, M., Solano, L., Barón, M. A., Arguello, F., Llovera, D., Ramirez, J., Leets, I. & Tropper, E. (1997) The role of vitamin A on the inhibitors of nonheme iron absorption: Preliminary results J. Nutr. Biochem. 8: 61-67.
20. Levin M. (1993) Cellular retinol-binding proteins are determinants of retinol uptake and metabolism in stably transfected Caco-2 cells, J. Biol. Chem 268: 8267-8276.
21. Levin, M., Talkad, V., Gordon, J. & Stenson, W. (1992) Trafficking of exogenous fatty acids within Caco-2 cells. J. Lipid Res. 33: 9-19.
22. Lissoos, T., Davis, A. & Levin, M. (1995) Vitamin A trafficking in Caco-2 cells stably transfected with cellular retinol binding proteins. Am. J. Physiol. 268 (Gastrointest. Liver phisiol. 31): G224-G231.
23. Mejia, L. & Arroyave G. (1982a) The effect of vitamin A fortification of sugar on iron metabolism in preschool children in Guatemala. Am. J. Clin. Nutr. 36:87-93.
24. Mejia, L., Arroyave G. (1982b) La interrelatión biológica entre vitamina A y hierro. Arch. Lat. Nutr. 32: 32-37.
25. Pinto, M., Robine-Leon, S., Appay, M., Kedinger, M., Triadou, N., Dussaulx, E., Lacroix, B., Simon-Assmann, P., Haffen, K., Fogh, J. & Zweilbaum, A. (1983) Enterocyte-like differentiation and polarization of the human colon carcinoma cell line Caco-2 in culture. Biol. Cell 47: 323-330.
26. Quick, T. & Ong, D. (1990) Vitamin A metabolism in the human intestinal Caco-2 cell line. Biochem 29: 11116-11123.
27. Raffaniello, R. & Wapnir, R. (1991) Zinc-induced metallothionein synthesis by Caco-2 cells. Bioch. Med. Metab. Biol. 45: 101-107.
28. Raymond, M., Martinot-Peignoux, M. & Erlinger, S. (1985) Dome formation in the human colon carcinoma cell line Caco-2 in culture. Influence of ouabain and permeable supports. Biol. Cell 54: 89-92.
29. Rousset, M. (1986) The human colon carcinoma cell lines HT-29 and Caco-2: two in vitro models for the study of intestinal differentiation. Biochimie 68: 1035-1040.
30. Sure, B., Kik, M. & Walker, D. (1992) The effect of avitaminosis on hematopoietic function I. Vitamin A deficiency. J. Biol. Chem 83: 375-385.
31. Trotter, P. & Storch, J. (1993) Nutritional control of fatty acid esterification in differentiating Caco-2 intestinal cells is mediated by diacylglycerol concentrations. J. Nutr. 123: 728-736.
32. Vanchon, P. & Beaulieu, J. F. (1992) Transient mosaic patterns of morphological and functional differentiation in the Caco-2 cell line. Gastroenterology 103: 414-423.
33. Vincent, M., Russell, R. & Sasak, V. (1985) Folic acid uptake characteristics of a human colon carcinoma cell line, Caco-2. A newly described cellular model for small intestinal epithelium. Hum. Nutr. Clin. Nutr. 39C: 355-360.