Method for determining protein kinase substrate specificities by the phosphorylation of peptide libraries on beads, phosphate-specific staining, automated sorting, and sequencing

Analytical Biochemistry

Volumn 276, Issue 2, 1999, Pages 227-241

  Gast, Rainer ^a   Glökler, Jörn ^a   Höxter, Maria ^a   Kieß, Michael ^a   Frank, Ronald ^a   Tegge, Werner ^a  

^a HELMHOLTZ CENTRE FOR INFECTION RESEARCH   (Germany)

Author keywords

Peptide libraries; Phosphate detection; Protein kinase specificity

Indexed keywords

AMINO ACIDS; FLUORESCENCE; IRON COMPOUNDS; LIBRARIES; PEPTIDES; PHOSPHORYLATION; SYNTHESIS (CHEMICAL);

FLUORESCENCE-ACTIVATED CELL SORTERS; IMMOBILIZED PEPTIDE; PEPTIDE LIBRARY; PEPTIDE SUBSTRATES; PHOSPHATE DETECTIONS; PHOSPHATE MONOESTER; PROTEIN KINASE; SOLID PHASE PEPTIDE SYNTHESIS;

ENZYMES;

PROTEIN KINASE;

AMINO ACID SEQUENCE; ARTICLE; ENZYME ACTIVITY; ENZYME MECHANISM; ENZYME SPECIFICITY; PRIORITY JOURNAL; PROTEIN PHOSPHORYLATION; PROTEIN STRUCTURE; SEQUENCE ANALYSIS;

EID: 0343851531     PISSN: 00032697     EISSN: None     Source Type: Journal    
DOI: 10.1006/abio.1999.4285     Document Type: Article

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