Dengue virus envelope glycoprotein can be secreted from insect cells as a fusion with the maltose-binding protein

Journal of Virological Methods

Volumn 56, Issue 2, 1996, Pages 179-189

  Staropoli, I ^a   Clement J M ^a   Frenkiel, M P ^a   Hofnung, M ^a   Deubel, V ^a  

^a INSTITUT PASTEUR   (France)

Author keywords

Baculovirus; Dengue virus; Envelope glycoprotein; Maltose binding protein; Purification; Secretion

Indexed keywords

HYBRID PROTEIN; MALTOSE BINDING PROTEIN; VIRUS GLYCOPROTEIN;

ANIMAL CELL; ARTICLE; BACULOVIRUS; CONTROLLED STUDY; DENGUE VIRUS; INSECT; LEPIDOPTERA; MOLECULAR CLONING; NONHUMAN; PRIORITY JOURNAL; PROTEIN PROCESSING; PROTEIN SECRETION; VIRUS ENVELOPE;

EID: 0343831399     PISSN: 01660934     EISSN: None     Source Type: Journal    
DOI: 10.1016/0166-0934(95)01953-7     Document Type: Article

References (24)

1. Clément, J.-M. and Popescu, O. (1991) MalE as a tool for the production of heterologous proteins in Escherichia coli. Bull. Inst. Pasteur 89, 243-253.
2. Delenda, C., Frenkiel, M.P. and Deubel, V. (1994a) Protective efficacy in mice of a secreted form of recombinant dengue-2 envelope protein produced in baculovirus-infected insect cells. Arch. Virol. 139, 197-207.
3. Delenda, C., Staropoli, I., Frenkiel, M.-P., Cabanié, L. and Deubel, V. (1994b) Analysis of C-terminally truncated dengue 2 and dengue 3 virus envelope glycoproteins: processing in insect cells and immunogenic properties in mice. J. Gen. Virol. 75, 1569-1578.
4. Desprès, P., Cahour, A., Wychowski, C., Girard, M. and Bouloy, M. (1988) Expression of yellow fever virus envelope protein using hybrid SV40/yellow fever viruses. Ann. Inst. Pasteur 139, 59-67.
5. Deubel, V., Kinney, R.M. and Trent, D.W. (1986) Nucleotide sequence and deduced amino acid sequence of the structural proteins of dengue type 2 virus, Jamaica genotype. Virology 165, 234-244.
6. Feighny, R., Burrous, J., McCown, J., Hoke, C. and Putnak, R. (1992) Purification of native dengue-2 viral proteins and the ability of purified proteins to protect mice. Am. J. Trop. Med. Hyg. 47, 405-412.
7. Flamand, M., Deubel, V. and Girard, M. (1992) Expression and secretion of Japanese encephalitis virus nonstructural protein NS1 by insect cells using a recombinant baculovirus. Virology 191, 826-836.
8. Gentry, M.K., Henchal, E.A., McCown, J.M., Brandt, W.E. and Dalrymple, J.M. (1982) Identification of distinct antigenie determinants of dengue 2 virus using monoclonal antibodies. Am. J. Trop. Med. Hyg. 31, 548-555.
9. Hofnung, M. and Charbit, A. (1993) Expression of antigens as recombinant proteins. In: M.H.V. Van Regenmortel (Ed), Structure of Antigens, Vol. 2, Chapter 4, pp. 79-128, CRC Press Inc./The Telford Press, NJ, USA.
10. Johnson, A.J., Guirakhoo, F. and Roehrig, J.T. (1994) The envelope glycoproteins of dengue 1 and dengue 2 grown in mosquito cells differ in their utilization of potential glycosylation sites. Virology 203, 241-249.
11. Leclerc, C., Dériaud, E., Mégret, F., Briand, J.P., Van Regenmortel, M.H.V. and Deubel, V. (1993) Identification of helper T cell epitopes of dengue virus E-protein. Mol. Immunol. 30, 613-625.
12. Luckow, V.A. and Summers, M.D. (1988) Trends in the development of baculovirus expression vectors. Bio/Technology 6, 47-55.
13. Maina, C.V., Riggs, P.D., Grandea, A.G. III, Slatko, B.E., Moran, L.S., Tagliamonte, J.A., McReynolds, L.A. and di Guan, C. (1988) An Escherichia coli vector to express and purify foreign proteins by fusion to and separation from maltose-binding protein. Gene 74, 365-373.
14. Martineau, P., Guillet, J.-G., Leclerc, C. and Hofnung, M. (1991) Expression of heterologous peptides at two permissive sites of the MalE protein: antigenicity and immunogenicity of foreign B- and T-cell epitopes. Gene 113, 35-46.
15. Mégret, F., Hugnot, J.P., Falconar, A., Gentry, M.K., Morens, D.M., Murray, J.M., Schlesinger, J.J., Wright, P.J., Young, P., Van Regenmortel, M.H.V. and Deubel, V. (1992) Use of recombinant fusion proteins and monoclonal antibodies to define linear and discontinuous antigenic sites on the dengue envelope glycoprotein. Virology 187, 480-491.
16. Monath, T.P. (1994) Dengue: The risk to developed and developing countries. Proc. Natl. Acad. Sci. USA 91, 2395-2400.
17. Nagia, K. and Thogersen, H.C. (1984) Generation of β-globin by sequence-specific proteolysis of a hybrid protein produced in Escherichia coli. Nature 309, 810-812.
18. Roehrig, J.T., Risi, P.A., Brubaker, J.R., Hunt, A.R., Beaty, B.J., Trent, D.W. and Mathews, J.H. (1994) T-helper epitopes on the E-glycoprotein of dengue 2 Jamaica virus. Virology 198, 31-38.
19. Sisk, W.P., Bradley, J.D., Leipold, R.J., Stoltzfus, A.M., Ponce de leon, M., Hilf, M., Peng, C., Cohen, G.H. and Eisenberg, R.J. (1994) High-level expression and purification of secreted forms of herpes simplex virus type 1 glycoprotein gD synthesized by baculovirus-infected insect cells. J. Virol. 68, 766-775.
20. Smith, G.W. and Wright, P.J. (1985) Synthesis of proteins and glycoproteins in dengue type 2 infected Vero and Aedes albopictus cells. J. Gen. Virol. 66, 559-571.
21. Spurlino, J.C., Lu, G.-Y. and Quiocho, F.A. (1991) The 2.3-A resolution structure of the maltose- or maltodextrin-binding protein, a primary receptor of bacterial active transport and chemotaxis. J. Biol. Chem. 266, 5202-5219.
22. Szmelcman, S., Clément, J.M., Jehanno, M., Schwartz, O., Montagnier, L. and Hofnung, M. (1990) Export and one-step purification from Escherichia coli of a MalE-CD4 hybrid protein that neutralizes HIV in vitro. J. AIDS 3, 859-872.
23. Uhlen, M., Forsberg, G., Moks, T., Hartmanis, M. and Nilson, B. (1992) Fusion proteins in biotechnology. Curr. Opin. Biotech. 3, 361-369.
24. Walker, P.A., Leog, L.E., Ng, P.W.P., Tan, S.H., Waller, S., Murphy, D. and Portier, A.G. (1994). Efficient and rapid affinity purification of proteins using recombinant fusion proteases. Bio/Technology 12, 601-605.