Enzyme-polyelectrolyte complexes in water-ethanol mixtures: Negatively charged groups artificially introduced into α-chymotrypsin provide additional activation and stabilization effects

Biotechnology and Bioengineering

Volumn 55, Issue 2, 1997, Pages 267-277

  Kudryashova, Elena V ^a   Gladilin, Alexander K ^a   Vakurov, Alexander V ^a   Heitz, Frederic ^b   Levashov, Andrey V ^a   Mozhaev, Vadim V ^a,c  

^a MOSCOW STATE UNIVERSITY   (Russian Federation)

^b ETABLISSEMENT FRANÇAIS DU SANG   (France)

^c UNIVERSITY OF IOWA   (United States)

Author keywords

chymotrypsin; Covalent modification; Denaturation; Enzymatic activity; Enzyme polyelectrolyte complexes; Water cosolvent mixtures

Indexed keywords

BINARY MIXTURES; CATALYST ACTIVITY; CHARGED PARTICLES; CHEMICAL BONDS; CHEMICAL MODIFICATION; COMPOSITION EFFECTS; CONFORMATIONS; ETHANOL; MOLECULAR WEIGHT; POLYELECTROLYTES; SOLUTIONS;

CHYMOTRYPSIN (CY); CIRCULAR DICHROISM (CD) METHOD; POLYBRENE (PB);

ENZYME KINETICS;

BINDING PROTEIN; CHYMOTRYPSIN A; POLYCATION;

ADAPTATION; ARTICLE; CATALYSIS; CHEMICAL MODIFICATION; COMPLEX FORMATION; COVALENT BOND; ENZYME ACTIVATION; ENZYME ACTIVITY; ENZYME DENATURATION; ENZYME SPECIFICITY; ENZYME STABILITY; ENZYME SUBSTRATE; NONHUMAN; PROTEIN PROTEIN INTERACTION;

EID: 0343812088     PISSN: 00063592     EISSN: None     Source Type: Journal    
DOI: 10.1002/(SICI)1097-0290(19970720)55:2<267::AID-BIT4>3.0.CO;2-G     Document Type: Article

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