Evaluation of patterns of urinary proteins by SDS-PAGE in rats of different ages

Mechanisms of Ageing and Development

Volumn 87, Issue 1, 1996, Pages 1-13

  Calzada García, J Arturo ^a   Pérez González, M Nieves ^a   Benito Andrés, F Javier ^a   García, Luis J Morales ^a   Cabezas, Jose A ^a   Sánchez Bernal, Carmen ^a  

^a UNIVERSITY OF SALAMANCA   (Spain)

Author keywords

Age; Proteinuria; Rat; SDS PAGE; Western blotting

Indexed keywords

ALBUMIN; CARBONATE DEHYDRATASE; IMMUNOGLOBULIN G; OROSOMUCOID; TAMM HORSFALL GLYCOPROTEIN; TRANSFERRIN;

AGING; ARTICLE; DENSITOMETRY; MALE; MOLECULAR WEIGHT; NEWBORN; NONHUMAN; POLYACRYLAMIDE GEL ELECTROPHORESIS; PRIORITY JOURNAL; PROTEIN URINE LEVEL; PROTEINURIA; RAT; SILVER STAINING; URINALYSIS;

EID: 0343807225     PISSN: 00476374     EISSN: None     Source Type: Journal    
DOI: 10.1016/0047-6374(96)01693-4     Document Type: Article

References (23)

1. A. Bianchi-Bosisio, F. D'agrosa, F. Gaboardi, E. Gianazza and G. Righetti, Sodium dodecyl sulphate electrophoresis of urinary proteins. J. Chromatogr., 569 (1991) 243-260.
2. R. Beetham and W.R. Cattell, Proteinuria: pathophysiology, significance and recommendations for measurement in clinical practice. Ann. Clin. Biochem., 30 (1993) 425-434.
3. R.S. Goldstein, J.B. Tarloff and J.B. Hook, Age-related nephropathy in laboratory rats. FASEB J., 2 (1988) 2241-2251.
4. J.M. López-Novoa and I. Montañés, Changes in renal function and morphology in aging laboratory animals. In J.F. Macías Núñez and J. Stewart Cameron (eds.), Renal function and diseases in the Elderly, Butterworth, Guildford, 1987, pp. 162-183.
5. C. Sánchez-Bernal, A. Martín-González, J.A. Cabezas, E. Villar and N. Pérez-González, Age-related excretion of six glycosidases in rat urine. Mech. Ageing Dev., 66 (1992) 121-129.
6. C.P. McCormick, Z.K. Shihabi, J.C. Konen, H.O. Goodman and D.A. Blizard, Microtransferrinuria and microalbuminuria II. In the rat. Clin. Physiol. Biochem., 8 (1990) 59-63.
7. A. Remuzzi, S. Puntorieri, M. Alfano, D. Macconi, M. Arbate, T. Bertani and G. Remuzzi, Pathophysiologic implications of proteinuria in a rat model of progressive glomerular injury. Lab. Invest., 67 (1992) 572-579.
8. J. Lubega, Evaluation of urinary proteins by dodecyl sulphate polyacrylamidc disc gel electrophoresis and molecular mass analysis. Clin. Chim. Acta, 128 (1983) 151-167.
9. J.J. Fleming, Comparison of tubular proteinuria, using sodium dodecyl sulphate polyacrylamide gel electrophoresis in patients during methotrexate or aminoglycoside treatment or with cadmium or Balkan nephropathy. Clin. Chim. Acta, 140 (1984) 267-277.
10. B. Kshirsagar and R.C. Wiggins, A map of urine proteins based on one-dimensional SDS-polyacrylamide gel electrophoresis and Western blotting using one microliter of unconcentrated urine. Clin. Chim. Acta, 158 (1986) 13-22.
11. S. Raicevic, S. Trnacevic, J. Hranisavljevic and D. Vucelic, Renal function, protein excretion, and pathology of Balkan endemic nephropathy. II. Protein excretion. Kidney Int., 40 (1991) 52-56.
12. K.S. Shiba, K. Kanamori, H. Cho, N. Furuhata, T. Harada, A. Shiba and M. Nakao, Re-evaluation of normal human urinary proteins fractionated by one-dimensional sodium dodecyl sulphate-polyacrylamide gel electrophoresis. Clin. Chim. Acta, 172 (1988) 77-84.
13. S. Gordon, R.D. Tee and A.J.N. Taylor, Analysis of rat urine proteins and allergens by sodium dodecyl sulfate-polyacrylamide gel electrophoresis and immunoblotting. J. Allergy Clin. Immunol., 92 (1993) 298-305.
14. U.K. Laemmli, Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature, 227 (1970) 680-685.
15. J.H. Morrissey, Silver stain for proteins in polyacrylamide gel. A modified procedure with enhanced uniform sensitivity. Anal. Biochem., 117 (1981) 307-310.
16. H. Towbin, T. Staehelin and J. Gordon, Electrophoretic transfer of protein from polyacrylamide gels to nitrocellulose sheet; procedure and some applications. Proc. Natl. Acad. Sci. USA., 9 (1976) 4350-4354.
17. I. Ala-Houhala, M.T. Parviainen and A. Pasternack, A comparison of three different methods of concentration of urinary proteins. Clin. Chim. Acta, 142 (1984) 339-342.
18. R.C. Wiggins, B. Kshirsagar, R.C. Kelsch and B.S. Wilson, Fragmentation and polymeric complexes of albumin in human urine. Clin. Chim. Acta, 149 (1985) 155-163.
19. C. Oliver, M. Peña, M.R. Sánchez, J.A. Hurtado and M. Samaniego, Electroforesis de proteínas y microproteínas en orina como valoración de la función renal en el periodo neonatal. XIII Congreso Nacional de la SEQC, 13 (1994) 394.
20. O.W. Neuhaus and W. Flory, Age-dependent changes in the excretion of urinary proteins by the rat. Nephron, 22 (1978) 570-576.
21. H.H. Reinhart, N. Obedeanu, R. Robinson, O. Korzeniowski, D. Kaye and J.D. Sobel, Urinary excretion of Tamm-Hörsfall protein in elderly women. J. Urol., 146 (1991) 806-808.
22. H. Yokoyama, Y. Uchigata, T. Otani, M. Tomioka, K.A. Kodama and Y. Omory, Changes of albumin concentration in the first morning urine according to age and sex in 2990 healthy children and adults. Diabetes Res. Clin. Prat., 21 (1993) 167-170.
23. A.L. Cessac, M. Perichon, J. Schaeverbeke and H. Bakala, Age-related changes in albumin binding by renal brush-border membrane vesicles. Mech. Ageing Dev., 70 (1993) 139-148.