Immobilized metal affinity chromatography of human growth hormone. Effect of ligand density

Journal of Chromatography A

Volumn 764, Issue 1, 1997, Pages 27-33

  Liesiene, J ^a   Racaityte K ^a   Morkeviciene M ^b   Valancius P ^b   Bumelis, V ^b  

^a KAUNAS UNIVERSITY OF TECHNOLOGY   (Lithuania)

^b UAB Biofa Graiciuno 8   (Lithuania)

Author keywords

Growth hormones; Immobilized metal affinity chromatography; Ligand density; Proteins; Somatotropin

Indexed keywords

HUMAN GROWTH HORMONE;

AFFINITY CHROMATOGRAPHY; ARTICLE; DRUG MANUFACTURE; ELECTROPHORESIS; ESCHERICHIA COLI; GENE EXPRESSION; PRIORITY JOURNAL; PROTEIN PURIFICATION; TECHNIQUE;

CHROMATOGRAPHY, AFFINITY; ELECTROPHORESIS, POLYACRYLAMIDE GEL; HUMAN GROWTH HORMONE; HUMANS; LIGANDS; METALS; RECOMBINANT PROTEINS;

ESCHERICHIA COLI;

EID: 0343742643     PISSN: 00219673     EISSN: None     Source Type: Journal    
DOI: 10.1016/S0021-9673(96)00869-2     Document Type: Article

References (13)

1. E. Sulkowski, Trends Biotechnol., 3 (1985) 1.
2. J. Porath, J. Carlston, I. Olsson and G. Belfrage, Nature, 258 (1975) 598.
3. L. Andersson, E. Sulkowski and J. Porath, Cancer Res., 47 (1987) 3624.
4. E. Sulkowski, Protein Purification: Micro to Macro, UCLA Symposium on Molecular and Cellular Biology, Frisco, CO, March 29-April 4, 1987, New Series, Vol. 68, Alan R. Liss, New York. NY, 1987, p. 149.
5. G. Muszynska, L. Andersson and J. Porath, Biochemistry, 25 (1986) 6850.
6. A. Šėrys, J. Liesienė, J. Urbonavičienė, A. Maruška, K. Radzevičius, D. Valatkevičiūtė and V. Bumelis, J. Liq. Chromatogr., 17 (1994) 749.
7. P.M. Rubcov, A.Sh. Parsadamian, P.S. Sverdlova, L.V. Lashas, K.G. Skriabin and A.A. Baev, Doklady Akademii Nauk SSSR, 276 (1984) 762.
8. A. Maruška, J. Liesienė and A. Šėrys, Lithuanian Pat., 2299 (1993).
9. U.K. Laemmli and M. Farze, J. Mol. Biol., 80 (1973) 575.
10. H. Flodh, Acta Paediatr. Scand. (Suppl.), 325 (1986) 1.
11. S. Lefort and P. Ferrara, J. Chromatogr., 361 (1986) 209.
12. F. Maisano, S.A. Testori and G. Grandi, J. Chromatogr., 472 (1989) 422.
13. K.C. Olson, J. Fenno, N. Lin, R.N. Harkins, C. Snider, W.H. Kohr, M.J. Ross, D. Fodge, G. Prender and N. Stebbing, Nature, 293 (1981) 408.