Asymmetric hydrolysis of enol esters with two esterases from Marchantia polymorpha

Tetrahedron Asymmetry

Volumn 11, Issue 5, 1999, Pages 1063-1066

  Hirata, Toshifumi ^a   Shimoda, Kei ^a   Kawano, Tsuyoshi ^a  

^a HIROSHIMA UNIVERSITY   (Japan)

Author keywords

[No Author keywords available]

Indexed keywords

ENOL ACETATE; ENOL ESTER; ESTER; ESTERASE; KETONE; UNCLASSIFIED DRUG;

CELL CULTURE; CHIRALITY; CONTROLLED STUDY; ENANTIOMER; HYDROLYSIS; MARCHANTIA POLYMORPHA; PLANT; PLANT CELL; PRIORITY JOURNAL; PROTON TRANSPORT; SHORT SURVEY; STEREOCHEMISTRY;

EID: 0343566447     PISSN: 09574166     EISSN: None     Source Type: Journal    
DOI: 10.1016/S0957-4166(00)00046-X     Document Type: Article

References (34)

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25. Homogenates of the cultured cells of M. polymorpha in 100 mM phosphate buffer (pH 7.0) were centrifuged at 100 000 g to give a cell-free extract, which was treated with ammonium sulfate (60-80% satd) to give a crude enzyme preparation. Butyl-Toyopearl column chromatography of the crude enzyme preparation gave a good separation of the two different esterases. Further purification by chromatography on a diethylaminoethyl-Toyo-pearl column and then a Sephadex G-75 column gave homogeneous esterases as judged by SDS-PAGE: esterase I, molecular mass ca. 54 000, dimeric form composed of two identical 27 000 subunits; esterase II, molecular mass ca. 45 000, dimeric form composed of two identical 22 500 subunits.
26. 8
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34. -1). Retention times for the products in the GLC were as follows: 8 and 15, 11.8 and 12.8 min; 9 and 16, 12.7 and 12.9 min; 10 and 17, 23.8 and 24.0 min; 11 and 18, 18.1 and 18.4 min; 12 and 19, 27.7 and 27.9 min; 13 and 20, 72.1 and 72.8 min; 14 and 21, 60.1 and 61.2 min.