Attachment of 3T3 and MDBK cells onto poly(EGDMA/HEMA) based microbeads and their biologically modified forms

Artificial Cells, Blood Substitutes, and Immobilization Biotechnology

Volumn 28, Issue 2, 2000, Pages 155-171

  Ayhan, H ^a   Gurhan I ^b   Piskin E ^a  

^a HACETTEPE UNIVERSITY   (Turkey)

^b Foot and Mouth Disease Institute   (Turkey)

Author keywords

3T3 and MDBK Cell Lines; Biological modification; Cell attachment; Collagen; Fibronectine; Mathematical simulation of attachment kinetics; Poly(EGDMA HEMA) based microbeads

Indexed keywords

1,6 HEXANEDIAMINE; COLLAGEN; FIBRONECTIN; GLUTARALDEHYDE; HYDROXYL GROUP; TOLUENE;

ANIMAL CELL; ARTICLE; CELL ADHESION; CELL STRAIN 3T3; CHEMICAL MODIFICATION; CONTROLLED STUDY; MATHEMATICAL MODEL; MOUSE; NONHUMAN; POLYMERIZATION; POROSITY; PROTEIN IMMOBILIZATION;

ANIMALIA;

EID: 0343550391     PISSN: 10731199     EISSN: None     Source Type: Journal    
DOI: 10.3109/10731190009118578     Document Type: Article

References (36)

1. Spier, R.E. and Clarke, J.B., Variation in the susceptibility of Bhk populations and cloned cell-lines to 3 strains of foot-and-mouth-disease virus, Archives of Virology, 1980;63,1-9.
2. Spier, R.E. and Griffith, B., (Eds.), 1985, Animal Cell Biotechnology, Academic Press, London.
3. Hu W-S., and Peshwa, M.V., Animal cell bioreactor-Recent advances and challenges to scale-up, Can. J. Chem. Eng., 1991; 69: 409-418.
4. Levine, D.W., Wang D.I.C., and Thilly, W.G., Optimization of growth surface parameters in microcarrier cell culture, Biotechnol. and Bioengineering, 1979;25:821-826.
5. Nilsson, K., and Mosbach, K., Preparation of immobilized animal cells, FEBS Lett., 1980; 118: 145-150.
6. Keese C.R., and Giaever, I., Cell-growth on liquid microcarriers, Science, 1983; 219: 1448-1449.
7. Kiremitçi, M., Özilgen, M., and Pişkin, E., Attachment and growth kinetics of anchorage-dependent BHK cells on microcarriers. Enzyme Microb.Technol., 1989; 11: 830-836.
8. Looby, D., and Griffiths, B., Immobilization of animal cells in porous carrier culture, Trends Biotechnol., 1990; 8: 204-208.
9. Reiter, M., Hohenwarter, O., Gaida, T., Zach, N., Schmartz, C., Cluml, G., Weigan, F., Nilsson, K., and Katinger, H., 1990, The use of macroporous gelatin carriers for the cultivation of mammalian cells in fluidized bed reactors, Cytotechnol., 3: 271-277.
10. Nikolai, T.J., and Hu, W-S.,Cultivation of mammalian cells on microporous microcarriers, Enzyme Microb.Technol., 1992; 14: 203-208.
11. Pişkin, E., Kiremitçi, M., Denkbaş, E.B., Gurhan, I., W.R. Gombotz, W.R., and A.S. Hoffman, A.S., Cell culturing on polymeric beads, Clinical Materials, 1992; 11: 171-178.
12. Dixit, V., Pişkin, E., Arthur, M., Denizli, A., Tuncel, S.A., Denkbaş, E.B., and Gitnick, G., Hepatocyte immobilization on PHEMA microcarriers and its biologically modified forms, Cell Transplant., 1992; 1: 391-399.
13. Roder, B., Zuhlke, A., Widdecke, H., and Klein, J., Synthesis and application of New microcarriers for animal cell culture. 2. Application of polystrene microcarriers, J. Biomaterial Sci. Polym. Ed., 1993; 5: 79-88.
14. Hawboldt, K.A., Kalogerakis, N., and Behie, L.A., A cellular-automaton model for microcarrier cultures, Biotechnology and Bioengineering, 1994; 43: 90-100.
15. Denkbaş, E.B., Hoffman, A.S., and Pişkin, E., Silicone-based microcarriers: preparation and BHK cell culture, Chemical Engineering J., 1995; 58: 65-70.
16. Tuncel, A., and Pişkin, E., Nonswellable and Swellable Poly(EGDMA) Microspheres, J. Appl. Polym. Sci., 1996; 62: 789-798.
17. Tuncel, A., Ecevit, K., Kesenci, K., Pişkin, E., Nonswellable and Swellable Etylene Glycol Dimethacrylate-Acrylic Acid Copolymer Microspheres, J. Appl. Polymer Sci., 1996; 34: 45-55.
18. Kesenci, K., Tuncel, A., and Pişkin, E., Swellable Ethylene Glycol Dimethacrylate-Hydroxyethylmethacrylate Copolymer Microspheres, Reactive Funct. Polymer Sci., 1996; 31: 137-147.
19. Denizli, A., Salih, B., and Pişkin, E., Cibacron Blue F3GA and Zn(II) Containing Poly(EGDMA-HEMA) Microbeads for Albumin Adsorption, J. M.S., Pure Appl. Chem., 1997; 34: 1983-1988.
20. Kocakulak, M., Denizli, A., Rad, A.Y., Pişkin, E., New sorbent for bilirubin removal from human plasma: Cibocron Blue F3GA-immobilized P(EGDMA-HEMA) microbeads, J. Chromatogr. B, 1997; 693: 271-276.
21. Ayhan, H., and Pişkin, E., A Potential Cell Affinity Sorbent: Fibronectine Carrying Poly(EGDMA/HEMA) Microbeads, J. of Biomaterial Sci. Polymer Ed., 1998; (in press).
22. Hou, K.C., Zaniewski, R., and Roy, S., Protein A immobilized affinity cartridge for immunoglobulin purification, Biotech. and Appl. Biochem., 1991; 13: 257-262.
23. Pişkin, E., Ayhan, H., Bulmuş, V., Rad, A.Y., Falkenhagen, D., and Weber, C., Protein A carrying monosize PMMA microbeads for the removal of HIgG from human plasma, Int. J. Artif. Organs, 1996; 19: 124-127.
24. Ayhan, H., Specific Sorbents for Cell affinity, Ph.D. Thesis, Hacettepe University, Ankara, Turkey, 1997.
25. A. I. Vogel, A text-book of practical organic chemistry, Third edition, Longmans Press, London (1956).
26. Petterson, G.L., Review of the foline phenol protein quatitation method of lowry, Rosebrough, Farr and Randal; Review; Anal. Biochem., 1979; 100; 2; 201-220.
27. Petterson, G.L., Review of the foline phenol protein quatitation method of lowry, Rosebrough, Farr and Randal; Review; Anal. Biochem., 1979; 100; 2; 201-220.
28. Bulmuş, V., Ayhan, H., Pişkin, E., Modified PMMA Monosize Microbeads for Glucose Oxidase Immobilization, The Chemical Eng. Journal and Biochem. Eng., 1997; 65; 71-76.
29. Civerchio-Perez, L., Faris, B., LaPointe, G., Beldekas, J., Leibowitz, H., and Franzblau, C., Use of collagen-hydroxyethylmethacrylate hydrogels, Proc.Natl.Acad.Sci. (USA), 1980; 77: 2064-2071.
30. Kleinman, H.K., Klebe, R.J., and Martin, G.R., Role of collageneous matrices in the adhesion and growth of cells, J. Cell and Biol., 1981; 88: 473-486.
31. Pierschbacher, M.D., and Rouslahti, R., Cell attachment activity of fibronectin can be dublicated by small synthetic fragments for the molecule, Nature, 1984; 309: 30-38.
32. Lydon, H.J., Minett, T.W., and Tighe, B.J., Cellular interactions with synthetic polymer surfaces in culture, Biomaterials, 1985; 6: 396-402.
33. Denizli, A., Pişkin, E., Dixit, V., Arthur, M., and Gitnick, G., Collagen and Fibronectin Immobilization on PHEMA Microcarriers for Hepatocyte Attachment, Int. J. Artif. Organs, 1995; 18: 90-95.
34. Weiss, R.M., and Ollis, D.F., Extracellular microbial polysaccharides. 1. Substrate, biomass, and product kinetic-equations for batch Xanthan Gum fermentation, Biotechnology and Bioengineering, 1980; 22: 859-873.
35. Lam, J.L., and Ollis, D.F., Kinetics of Multiprotuct Fermentations, Biotechnology and Bioengineering, 1981; 23: 1517-1525.
36. Özilgen, M., Kinetics of amino-acid production by over-producer mutant microorganisms, Enzyme Microbial Technol., 1988; 10: 110-114.