Evaluation of the Roche Diagnostics LightCycler-factor V Leiden mutation detection kit and the LightCycler-prothrombin mutation detection kit

Clinical Biochemistry

Volumn 33, Issue 3, 2000, Pages 213-216

  Nauck, Markus ^a   März, Winfried ^a   Wieland, Heinrich ^a  

^a UNIVERSITY OF FREIBURG   (Germany)

Author keywords

[No Author keywords available]

Indexed keywords

AMINO ACID SEQUENCE; ARTICLE; DELETION MUTANT; DEVICE; DIAGNOSTIC ACCURACY; DIAGNOSTIC VALUE; DNA DETERMINATION; HUMAN; PRIORITY JOURNAL; VEIN THROMBOSIS;

DNA; DNA MUTATIONAL ANALYSIS; EVALUATION STUDIES; FACTOR V; FLUORESCENT DYES; GENOTYPE; HUMANS; MUTATION; NUCLEIC ACID HYBRIDIZATION; POLYMERASE CHAIN REACTION; POLYMORPHISM, RESTRICTION FRAGMENT LENGTH; PROTHROMBIN; REPRODUCIBILITY OF RESULTS; THROMBOSIS;

EID: 0343517715     PISSN: 00099120     EISSN: None     Source Type: Journal    
DOI: 10.1016/S0009-9120(00)00056-4     Document Type: Article

References (10)

1. Dahlback B., Hillarp A., Rosen S., Zoller B. Resistance to activated protein C, the FV:Q506 allele, and venous thrombosis. Ann Hematol. 72:1996;166-176.
2. Bertina R.M., Koeleman B.P., Koster T., et al. Mutation in blood coagulation factor V associated with resistance to activated protein C. Nature. 369:1994;64-67.
3. Poort S.R., Rosendaal F.R., Reitsma P.H., Bertina R.M. A common genetic variation in the 3′-untranslated region of the prothrombin gene is associated with elevated plasma prothrombin levels and an increase in venous thrombosis. Blood. 88:1996;3698-3703.
4. de Ronde H., Bertina R.M. Laboratory diagnosis of APC-resistance a critical evaluation of the test and the development of diagnostic criteria . Thromb Haem. 72:1994;880-886.
5. Kirschbaum N.E., Foster P.A. The polymerase chain reaction with sequence specific primers for the detection of the factor V mutation associated with activated protein C resistance. Thromb Haemost. 74:1995;874-878.
6. Hezard N., Cornillet-Lefebvre P., Gillot L., Potron G., Nguyen P. Multiplex ASA PCR for a simultaneous determination of factor V Leiden gene, G → A 20210 prothrombin gene and C → T 677 MTHFR gene mutations. Thromb Haemost. 79:1998;1054-1055.
7. Lay M.J., Wittwer C.T. Real-time fluorescence genotyping of factor V Leiden during rapid-cycle PCR. Clin Chem. 43:1997;2262-2267.
8. von Ahsen N., Schutz E., Armstrong V.W., Oellerich M. Rapid detection of prothrombotic mutations of prothrombin (G20210A), factor V (G1691A), and methylenetetrahydrofolate reductase (C677T) by real-time fluorescence PCR with the LightCycler. Clin Chem. 45:1999;694-696.
9. Aslanidis C., Nauck M., Schmitz G. High-speed prothrombin G → A 20210 and Methylenetetrahydrofolate Reductase C → T 677 mutation detection using real-time fluorescence PCR and melting curves. Biotechniques. 27:1999;234-236.
10. Chehab F.F., Kan Y.W. Detection of specific DNA sequences by fluorescence amplification a color complementation assay . Proc Natl Acad Sci USA. 86:1989;9178-9182.