19F NMR investigation of F1-ATPase of Escherichia coli using fluorotryptophan labeling

Journal of Biochemistry

Volumn 127, Issue 6, 2000, Pages 1053-1056

  Lee, Hean Woo ^a   Sohn, Joon Hyung ^a   Yeh, Byung Il ^a   Choi, Jong Whan ^a   Jung, Seunho ^b   Kim, Hyun Won ^a  

^a Yonsei University Wonju College of Medicine   (South Korea)

^b Konkuk University   (South Korea)

Author keywords

4 chloro 7 nitro benzofurazan; 19F NMR; Escherichia coli; F1 ATPase; Glyphosate

Indexed keywords

4 CHLORO 7 NITROBENZOFURAZAN; ADENOSINE TRIPHOSPHATASE; GLYPHOSATE; TRYPTOPHAN; CARBOXYPEPTIDASE; DIMETHYLAMINE; DRUG DERIVATIVE; FLUORINE; GLYCINE; N,N DIMETHYLDODECYLAMINE OXIDE; PROTON TRANSPORTING ADENOSINE TRIPHOSPHATASE;

AMINO ACID SYNTHESIS; ARTICLE; BACTERIAL GROWTH; CONFORMATIONAL TRANSITION; ENZYME ACTIVE SITE; ESCHERICHIA COLI; LIGAND BINDING; NONHUMAN; NUCLEAR MAGNETIC RESONANCE SPECTROSCOPY; BIOSYNTHESIS; CHEMISTRY; DRUG ANTAGONISM; ENZYMOLOGY; METABOLISM;

BACTERIA (MICROORGANISMS); ESCHERICHIA COLI;

4-CHLORO-7-NITROBENZOFURAZAN; CARBOXYPEPTIDASES; DIMETHYLAMINES; ESCHERICHIA COLI; FLUORINE; GLYCINE; MAGNETIC RESONANCE SPECTROSCOPY; PROTON-TRANSLOCATING ATPASES; TRYPTOPHAN;

EID: 0343517076     PISSN: 0021924X     EISSN: None     Source Type: Journal    
DOI: 10.1093/oxfordjournals.jbchem.a022697     Document Type: Article

References (17)

1. Post, J.F.M., Cottam, P.F., Simplaceanu, V., and Ho, C. (1984) Fluorine-19 nuclear magnetic resonance studies of 5-fluorotryptophan labeled histidine-binding protein J of Samonella typhimurium. J. Mol. Biol. 179, 729-743
2. Rule, G.S., Pratt, E.A., Simplaceanu, V., and Ho, C. (1987) Nuclear magnetic resonance and molecular genetic studies of the membrane-bound D-lactate dehydrogenase of Escherichia coli. Biochemistry 26, 549-556
3. 19F-n.m.r. studies of ligand binding to 5-fluorotryptophan-and 3-fluorotyrosine-containing cyclic AMP receptor protein from Escherichia coli. Biochem. J. 266, 545-552
4. Kim, H.-W., Perez, J.A., Ferguson, S.J., and Campbell, I.D. (1990) The specific incorporation of labeled aromatic amino acids into proteins through growth of bacteria in the presence of glyphosate. FEBS Lett. 272, 34-36
5. Steinrucken, H.C. and Amrhein, N (1980) The herbicide glyphosate is a potent inhibitor of 5-enolpyruvyl-shikimic acid-3-phosphate synthase. Biochem. Biophys. Res. Commun. 94, 1207-1212
6. Steinrucken, H.C. and Amrhein, N. (1984) 5-Enolpyruvylshikimate-3-phosphate synthase of Klebsiella pneumoniae 2. Inhibition by glyphosate [N-(phosphonomethyl)glycine]. Eur. J. Biochem. 143, 351-357
7. Boyer, P.D. (1993) The binding change mechanism for ATP synthase-some probabilities and possibilities. Biochim. Biophys. Acta 1140, 215-250
8. 1-ATPase of Escherichia coli using fluorinate ligands. J. Biochem. Mol. Biol. 31, 44-47
9. Walker J.E., Saraste, M., Runswick, M. and Gay, N.J. (1982) Distantly related sequences in the alpha-and beta-subunits of ATP synthase, myosin, kinases and other ATP-requiring enzymes and a common nucleotide binding fold. EMBO J. 1, 1947-1951
10. Senior, A.E., Falyle, D.R.H., Downie, J.A., Gibson, F., and Cox, G.B. (1979) Properties of membranes from mutant strains of Escherichia coli in which the beta-subunit of the adenosine triphosphatase is abnormal. Biochem. J. 180, 111-118
11. 1 adenosine triphophatase. Biochemistry 19, 5665-5670
12. Bradford, M.M. (1976) A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principles of protein-dye binding. Anal. Biochem. 72, 248-254
13. Ferguson, S.J., Lloyd, W.J., and Radda, G.K. (1975) The mitochondrial ATPase. Selective modification of a nitrogen residue in the beta subunit. Eur. J. Biochem. 54, 127-133
14. 1 by the detergent lauryldimethylamine oxide. Biochemistry 23, 4140-4143
15. Sutton, R. and Ferguson, S.J. (1985) Identification of an essential beta chain lysine residue from bovine heart mitochondrial ATPase specifically modified with nitrobenzofurazan. FEBS Lett. 179, 551-554
16. 14C]benzofurazan. J. Biol. Chem. 259, 8219-8225
17. Walker J.E., Saraste, M., and Gay, N.J. (1984) The une operon. Nucleotide sequence, regulation and structure of ATP-synthase. Biochim. Biophys. Acta 768, 164-200