Expression and purification of human matrilysin produced in baculovirus-infected insect cells

Journal of Biotechnology

Volumn 46, Issue 3, 1996, Pages 235-241

  López De Turiso, José A ^a   Fernández, Piedad ^a   Barbacid, M Mar ^a   Mira, Emilia ^a   Quesada, Ana R ^a   Márquez, Gabriel ^a   Aracil, Miguel ^a  

^a Departamento de Investigación   (Spain)

Author keywords

baculovirus; insect cells; matrilysin; matrix metalloproteinases

Indexed keywords

MATRILYSIN; MATRIX METALLOPROTEINASE; METALLOPROTEINASE;

ANIMAL CELL; ARTICLE; BACULOVIRUS; CONTROLLED STUDY; ENZYME PURIFICATION; GENE EXPRESSION; INSECT; MOLECULAR CLONING; NONHUMAN; PRIORITY JOURNAL;

ANIMALIA; INSECTA; UNIDENTIFIED BACULOVIRUS;

EID: 0343431391     PISSN: 01681656     EISSN: None     Source Type: Journal    
DOI: 10.1016/0168-1656(96)00002-8     Document Type: Article

References (21)

1. Barnett, J., Straub, K., Nguyen, B., Chow, J., Suttman, R., Thompson, K., Tsing, S., Benton, P., Schatzman, R., Chen, M. and Chan, H. (1994) Production, purification, and characterization of human matrilysin (Pump) from recombinant chinese hamster ovary cells. Protein Express. Purif. 5, 27-36.
2. Basset, P., Bellocq, J.P., Wolf, C., Stoll, I., Hutin, P., Limacher, J.M., Podhajcer, O.L., Chenard, M.P., Rio, M.C. and Chambon, P. (1990) A novel metalloproteinase gene specifically expressed in stromal cells of breast carcinomas. Nature (London) 348, 699-704.
3. Carrascosa, A.L. (1994) Enhancement of baculovirus plaque assay in insect cell monolayers by DEAE-dextran. Bio\Techniques 16, 1078-1085.
4. Fernández-Resa, P., Mira, E. and Quesada, A.R. (1995) Enhanced detection of casein zymography of matrix metalloproteinases. Anal. Biochem. 224, 434-435.
5. Liotta, L.A., Steeg, P.S. and Stetler-Stevenson, W.G. (1991) Cancer metastasis and angiogenesis: an imbalance of positive and negative regulation. Cell 64, 327-336.
6. Luckow, V.A. (1991) Cloning and expression of heterologous genes in insect cells with baculovirus vectors. In: Prokop, A., Bajpai, R.K. and Ho, C.S. (Eds.), Recombinant DNA Technology and Applications. McGraw-Hill, New York, pp. 97-152.
7. McDonnell, S., Navre, M., Coffey, R.J. and Matrisian, L.M. (1991) Expression and localization of the matrix metalloproteinase Pump-1 (MMP-7) in human gastric and colon carcinomas. Mol. Carcinogen. 4, 527-533.
8. Miyazaki, K., Hattori, Y., Umenishi, F., Yasumitsu, H. and Umeda, M. (1990) Purification and characterization of extracellular matrix-degrading metalloproteinase, matrin (Pump-1), secreted from human rectal carcinoma cell line. Cancer Res. 50, 7758-7764.
9. Muller, D., Breathnach, R., Engelmann, A., Millon, R., Bronner, G., Flesch, H., Dumont, P., Eber, M. and Abecassis, J. (1991) Expression of collagenase-related metalloproteinase genes in human lung or head and neck tumors. Int. J. Cancer 48, 550-556.
10. Muller, D., Quantin, B., Gesnel, M.C., Millon-Collard, R., Abecassis, J. and Breathnach, R. (1988) The collagenase gene family in humans consists of at least four members. Biochem. J. 253, 187-192.
11. Murphy, G., Cockett, M.I., Ward, R.V. and Docherty, A.J.P. (1991) Matrix metalloproteinase degradation of elastin, type IV collagen and proteoglycan. Biochem. J. 277, 277-279.
12. Nakano, A., Tani, E., Miyazaki, K., Furuyama, J. and Matsumoto, T. (1993) Expressions of matrilysin and stromelysin in human glioma cells. Biochem. Biophys. Res. Commun. 192, 999-1003.
13. Pajouh, M.S., Nagle, R.B., Breathnach, R., Finch, J.S., Brawer, M.K. and Bowden, G.T. (1991) Expression of metalloproteinase genes in human prostate cancer. J. Cancer Res. Clin. Oncol. 117, 144-150.
14. Powell, W.C., Knox, J.D., Navre, M., Grogan, T.M., Kittelson, J., Nagle, R.B. and Bowden, G.T. (1993) Expression of the metalloproteinase matrilysin in DU-145 cells increases their invasive potential in severe combined immunodeficient mice. Cancer Res. 53, 417-422.
15. Quantin, B., Murphy, G. and Breathnach, R. (1989) Pump-1 cDNA codes for a protein with characteristics similar to those of classical collagenase family members. Biochemistry 28, 5327-5334.
16. 73 active-site zinc complex in latency and a "cysteine switch" mechanism for activation. Proc. Natl. Acad. Sci. USA 87, 364-368.
17. Summers, M.D. and Smith, G.E. (1987) A manual of methods for baculovirus vectors and insect cell culture procedures. Texas Agricultural Experiment Station Bulletin No. 1555. College Station, Texas.
18. Witty. J.P., Farmanfarmaian, K., McDonnell, S. and Matrisian, L.M. (1992) Expression of the metalloproteinase matrilysin correlates with the invasive potential of human colon cancer cells in vitro. Proc. Am. Assoc. Cancer Res. 33, 59.
19. Witty, J.P., McDonnell, S., Newell, K.J., Cannon, P., Navre, M., Tressler, R.J. and Matrisian, L.M. (1994) Modulation of matrilysin levels in colon carcinoma cell lines affects tumorigenicity in vivo. Cancer Res. 54, 4805-4812.
20. Woessner, J.F., Jr. and Taplin, C.J. (1988) Purification and properties of a small latent matrix metalloproteinase of the rat uterus. J. Biol. Chem. 263, 16918-16925
21. Yoshimoto, M., Itoh, F., Yamamoto, H., Hinoda, Y., Imai, K. and Yachi, A. (1993) Expression of MMP-7 (Pump-1) mRNA in human colorectal cancers. Int. J. Cancer 54, 614-618.